Recombinant Human HR6B/UBE2B Protein

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SDS-Page: Human HR6B/UBE2B His (N-Term) Protein [NBP2-35088]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Func, PAGE
Format
Carrier-Free

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Catalog# & Formulation Size Price

Recombinant Human HR6B/UBE2B Protein Summary

Description
A single, non-glycosylated biologically active polypeptide chain corresponding to 152 residues of UBE2B with an N-terminal His-Tag.

Source: E. coli

Amino Acid Sequence:
MHHHHHHAMG QLRSMSTPAR RRLMRDFKRL QEDPPVGVSG APSENNIMQW NAVIFGPEGT PFEDGTFKLV IEFSEEYPNK PPTVRFLSKM FHPNVYADGS ICLDILQNRW SPTYDVSSIL TSIQSLLDEP NPNSPANSQA AQLYQENKRE YEKRVSAIVE QSWNDS

Preparation
Method
Novus' biologically active proteins are stringently purified to provide only the safest and most highly effective proteins available. This protein was expressed in E. coli, purified by HPLC, QC tested by SDS-PAGE and Western Blot and validated on appropriate cell lines for bioactivity. All HPLC and bioactivity data is provided for your assurance.
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
UBE2B
Purity
>95% pure by SDS-PAGE
Endotoxin Note
Less than 1EU/ug of endotoxin as determined by LAL method.

Applications/Dilutions

Theoretical MW
19.0 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
A 0.2 um filtered concentrated solution in 50 mM HEPES, pH 7.6 with 125 mM NaCl, 10 % Glycerol, 5 % Trehalose, 1 mM DTT.
Preservative
5% Trehalose
Purity
>95% pure by SDS-PAGE
Reconstitution Instructions
Please contact technical support for detailed reconstitution instructions.

Alternate Names for Recombinant Human HR6B/UBE2B Protein

  • E2 protein
  • E2-17k
  • E2-17kDa
  • HHR6B
  • HR6B
  • RAD6 homolog B
  • RAD6BEC 6.3.2.19
  • UBC2
  • UBE2B
  • Ubiquitin carrier protein B
  • ubiquitin-conjugating enzyme E2 B
  • Ubiquitin-conjugating enzyme E2-17 kDa
  • ubiquitin-conjugating enzyme E2B (RAD6 homolog)
  • Ubiquitin-protein ligase B

Background

This E2 enzyme encodes for the human homolog of the yeast DNA repair gene RAD6, which is induced by DNA damaging agents. UBE2B can conjugate ubiquitin to histone H2A in an E3- independent manner in vitro, and is essential for the multiubiquitination and degradation of N-end rule substrates. Additionally, UBE2B may have a role in sepsis-induced muscle protein proteolysis and cancer-induced cachexia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol UBE2B