HR6B/UBE2B Antibody

Western Blot: HR6B/UBE2B Antibody [NBP1-31293] - Mouse tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with UBE2B antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: HR6B/UBE2B Antibody [NBP1-31293] - Paraformaldehyde-fixed A431, using antibody at 1:500 dilution.

Western Blot: HR6B/UBE2B Antibody [NBP1-31293] - A. 30 ug 293T whole cell lysate/extract B. 30 ug whole cell lysate/extract of 3xFlag-human UBE2B-transfected 293T cells. Used at a dilution of 1:5000.

• Reactivity: Hu, Mu, Rt, Po, Bv, Rb, RM, Xp, Ze
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

HR6B/UBE2B Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human HR6B/UBE2B. The exact sequence is proprietary.
• Localization: Cell membrane , Nucleus
• Predicted Species: Rat (100%), Porcine (100%), Bovine (100%), Rabbit (100%), Rhesus Macaque (100%), Zebrafish (95%), Xenopus (98%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: UBE2B
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:10000
• Theoretical MW: 17 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (98%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol, 1% BSA
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for HR6B/UBE2B Antibody

• E2 protein
• E2-17k
• E2-17kDa
• HHR6B
• HR6B
• RAD6 homolog B
• RAD6BEC 6.3.2.19
• UBC2
• UBE2B
• Ubiquitin carrier protein B
• ubiquitin-conjugating enzyme E2 B
• Ubiquitin-conjugating enzyme E2-17 kDa
• ubiquitin-conjugating enzyme E2B (RAD6 homolog)
• Ubiquitin-protein ligase B

Background

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. Its protein sequence is 100% identical to the mouse, rat, and rabbit homologs, which indicates that this enzyme is highly conserved in eukaryotic evolution.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: UBE2B