Home » HPSC152 » HPSC152 Antibodies » HPSC152 Antibody

HPSC152 Antibody

 Submit a Review

Images

 
(
Enlarge)
Western Blot: HPSC152 Antibody [NBP3-03251] - Western blot analysis of extracts of various cell lines, using HPSC152 antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 90s.
Western Blot: HPSC152 Antibody [NBP3-03251] - Western blot analysis of extracts of various cell lines, using HPSC152 antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

100% Guarantee on every product

HPSC152 Antibody Summary

Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-125 of human HPSC152 (NP_057488.1).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TRMT112
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS with 50% glycerol, pH7.3.
Preservative
0.01% Thimerosal
Purity
Affinity purified

Alternate Names for HPSC152 Antibody

  • HSPC152
  • HSPC170
  • TRM112
  • TRM112-like protein
  • TRMT11-2
  • tRNA methyltransferase 11-2 homolog (S. cerevisiae)
  • tRNA methyltransferase 112 homolog

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Recombinant Human
TRM11 GST (N-Term) Pr ...
H00060487-P01
12.5% SDS-PAGE Stained with Coomassie Blue.
Species: Hu
Applications: ELISA, AP, PA, WB
ZFP36L1 Antibody
NBP2-86906
Immunohistochemistry-Paraffin: ZFP36L1 Antibody [NBP2-86906] - Rabbit Anti-ZFP36L1 Antibody. Formalin Fixed Paraffin Embedded Tissue: Human Adult heart. Observed Staining: Cytoplasmic. Primary Antibody Concentration: 1:600. Secondary Antibody: Donkey anti-Rabbit-Cy2/3.
Immunohistochemistry-Paraffin: ZFP36L1 Antibody [NBP2-86906] - Rabbit Anti-ZFP36L1 Antibody. Formalin Fixed Paraffin Embedded Tissue: Human Adult liver. Observed Staining: Cytoplasmic. Primary Antibody Concentration: 1:100. Secondary Antibody: Donkey anti-Rabbit-Cy2/3.
Immunohistochemistry-Paraffin: ZFP36L1 Antibody [NBP2-86906] - Rabbit Anti-ZFP36L1 Antibody. Formalin Fixed Paraffin Embedded Tissue: Human Liver Tissue. Observed Staining: Cytoplasm in smooth muscle cells in portal vein.
Species: Hu
Applications: IHC, IHC-P, WB
SLC7A5/LAT1
Antibody (BU53) - BSA
NBP2-50465
Immunohistochemistry-Paraffin: SLC7A5/LAT1 Antibody (BU53) [NBP2-50465] - Analysis of a FFPE tissue section of human testis using 1:200 dilution of SLC7A5/LAT1 (BU53) antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.
Flow (Cell Surface): SLC7A5/LAT1 Antibody (BU53) [NBP2-50465] - A cell surface stain was performed on Raji cells with SLC7A5 (BU53) antibody NBP2-50465 (blue) along with a matched isotype control NB600-986 (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at RT, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).
Flow (Cell Surface): SLC7A5/LAT1 Antibody (BU53) [NBP2-50465] - A cell surface stain was performed on Raji cells with SLC7A5 (BU53) antibody NBP2-50465AF647 (blue) along with a matched isotype control NB600-986AF647 (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at RT. Both antibodies were conjugated to Alexa Fluor 647.
(2 Publications)
Species: Hu
Applications: CyTOF-ready, Flow-CS, Flow, IHC, IHC-P
eRF1 Antibody
NBP1-31113
Immunohistochemistry-Paraffin: eRF1 Antibody [NBP1-31113] - Paraffin-embedded mouse prostate. eRF1 antibody diluted at 1:500. Antigen Retrieval: TrilogyTM (EDTA based) buffer, 15min
Immunohistochemistry-Paraffin: eRF1 Antibody [NBP1-31113] - Paraffin-embedded mouse testis. eRF1 antibody diluted at 1:500. Antigen Retrieval: TrilogyTM (EDTA based) buffer, 15min
Immunocytochemistry/Immunofluorescence: eRF1 Antibody [NBP1-31113] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: eRF1 protein stained by eRF1 antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200. Blue: Hoechst 33342 staining.
(2 Publications)
Species: Hu, Pm, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
BUD31 Antibody
NBP1-84010
Western Blot: BUD31 Antibody [NBP1-84010] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunohistochemistry-Paraffin: BUD31 Antibody [NBP1-84010] - Staining of human nasopharynx shows strong cytoplasmic and nuclear positivity in respiratory epithelial cells.
Immunocytochemistry/Immunofluorescence: BUD31 Antibody [NBP1-84010] - Staining of human cell line U-2 OS shows localization to nucleus & microtubules. Antibody staining is shown in green.
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
AP-2 gamma
Antibody [Unconjugate
AF5059
Western blot shows lysates of A431 human epithelial carcinoma cell line and human thymus tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human AP‑2 gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5059) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for AP‑2 gamma at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
AP‑2 gamma was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 10 µg/mL Goat Anti-Human AP‑2 gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5059) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
(3 Publications)
Species: Hu
Applications: IHC, WB
ZBTB12 Antibody
H00221527-B01P
Immunocytochemistry/Immunofluorescence: ZBTB12 Antibody [H00221527-B01P] - Analysis of purified antibody to ZBTB12 on HeLa cell. (antibody concentration 10 ug/ml)
Western Blot: ZBTB12 Antibody [H00221527-B01P] - Analysis of ZBTB12 expression in transfected 293T cell line by ZBTB12 polyclonal antibody. Lane 1: ZBTB12 transfected lysate(50.49 KDa). Lane 2: Non-transfected lysate.
Species: Hu
Applications: ICC/IF, WB
ELSPBP1 Antibody
NBP2-13957
Western Blot: ELSPBP1 Antibody [NBP2-13957] - Analysis in control (vector only transfected HEK293T lysate) and ELSPBP1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: ELSPBP1 Antibody [NBP2-13957] - Analysis in human epididymis and prostate tissues. Corresponding ELSPBP1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: ELSPBP1 Antibody [NBP2-13957] - Staining of human cerebral cortex shows no positivity in neurons as expected.
Species: Hu
Applications: IHC, IHC-P, WB

beta-Glucuronidase/GUS
NBP1-87511
Immunocytochemistry/Immunofluorescence: beta-Glucuronidase/GUSB Antibody [NBP1-87511] - staining of human cell line U-2 OS shows localization to vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: beta-Glucuronidase/GUSB Antibody [NBP1-87511] - Analysis in human liver and skeletal muscle tissues using NBP1-87511 antibody. Corresponding GUSB RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: beta-Glucuronidase/GUSB Antibody [NBP1-87511] - Staining of human liver shows strong positivity in lysosomes in hepatocytes.
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
HEMK2 Antibody
NBP3-03312
Western Blot: HEMK2 Antibody [NBP3-03312] - Analysis of extracts of Rat kidney, using N6AMT1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 90s.
Immunocytochemistry/Immunofluorescence: HEMK2 Antibody [NBP3-03312] - Analysis of PC-12 cells using N6AMT1 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Species: Hu, Mu, Rt
Applications: ICC/IF, WB
Glutathione
Peroxidase 1/GPX1 Ant ...
AF3798
Western blot shows lysates of human, mouse and rat liver tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat Glutathione Peroxidase 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3798) followed by HRP‑conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Glutathione Peroxidase 1 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Simple Western lane view shows lysates of human liver tissue and mouse liver tissue, loaded at 0.2 mg/mL. A specific band was detected for Glutathione Peroxidase 1/GPX1 at approximately 30 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat Glutathione Peroxidase 1/GPX1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3798) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.
Glutathione Peroxidase 1/GPX1 was detected in perfusion fixed frozen sections of rat liver using 15 µg/mL Goat Anti-Human/Mouse/Rat Glutathione Peroxidase 1/GPX1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3798) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP‑DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm in hepatocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
(15 Publications)
Species: Hu, Mu, Rt
Applications: IHC, KO, Simple Western, WB
DREF Antibody
(OTI5A2) - Azide and ...
NBP2-71419
Western Blot: DREF Antibody (OTI5A2) - Azide and BSA Free [NBP2-71419] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DREF.
Flow Cytometry: DREF Antibody (OTI5A2) - Azide and BSA Free [NBP2-71419] - Analysis of HEK293T cells transfected with either overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostaining by DREF1 antibody.
Immunohistochemistry: DREF Antibody (OTI5A2) - Azide and BSA Free [NBP2-71419] - Analysis of Adenocarcinoma of Human colon tissue.
Species: Hu
Applications: CyTOF-ready, Flow, IHC, WB
Recombinant Human
TRAM1 GST (N-Term) Pr ...
H00023471-P01
SDS-Page: Recombinant Human TRAM1 Protein [H00023471-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.
Species: Hu
Applications: ELISA, AP, PA, PAGE, WB
DR3/TNFRSF25
Antibody (59204) [Unc ...
MAB943
Mouse splenocytes either (A) untreated or (B) treated with PMA and Calcium Ionomycin for 24 hours were stained with Mouse Anti-Human DR3/TNFRSF25 Monoclonal Antibody (Catalog # MAB943) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B) and Rat Anti-Mouse CD3 PE‑conjugated Monoclonal Antibody (Catalog # FAB4841P). Quadrant markers were set based on control antibody staining (Catalog # MAB002).
Human peripheral blood mononuclear cells (PBMCs) either (A) untreated or (B) treated with PMA and Calcium Ionomycin for 24 hours were stained with Mouse Anti-Human DR3/TNFRSF25 Monoclonal Antibody (Catalog # MAB943) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B) and Mouse Anti-Human CD3 epsilon APC‑conjugated Monoclonal Antibody (Catalog # FAB100A). Quadrant markers were set based on control antibody staining (Catalog # MAB002).
Western blot shows lysates of human CD8+ T cells. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse DR3/TNFRSF25 Monoclonal Antibody (Catalog # MAB943) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for DR3/TNFRSF25 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
(2 Publications)
Species: Hu, Mu
Applications: CyTOF-ready, Flow, WB
ALKBH8 Antibody
NBP2-38928
Immunocytochemistry/Immunofluorescence: ALKBH8 Antibody [NBP2-38928] - Staining of human cell line U-2 OS shows positivity in nucleus.
Immunohistochemistry-Paraffin: ALKBH8 Antibody [NBP2-38928] - Staining of human lymph node shows cytoplasmic positivity in germinal center.
Species: Hu
Applications: ICC/IF, IHC, IHC-P
HPSC152 Antibody
NBP3-03251
HPSC152 Antibody
Species: Hu, Mu, Rt
Applications: WB

Publications for HPSC152 Antibody (NBP3-03251) (0)

There are no publications for HPSC152 Antibody (NBP3-03251).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for HPSC152 Antibody (NBP3-03251) (0)

There are no reviews for HPSC152 Antibody (NBP3-03251). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for HPSC152 Antibody (NBP3-03251) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional HPSC152 Products

Bioinformatics Tool for HPSC152 Antibody (NBP3-03251)

Discover related pathways, diseases and genes to HPSC152 Antibody (NBP3-03251). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for HPSC152 Antibody (NBP3-03251)

Discover more about diseases related to HPSC152 Antibody (NBP3-03251).
 

Pathways for HPSC152 Antibody (NBP3-03251)

View related products by pathway.

PTMs for HPSC152 Antibody (NBP3-03251)

Learn more about PTMs related to HPSC152 Antibody (NBP3-03251).
 

Research Areas for HPSC152 Antibody (NBP3-03251)

Find related products by research area.

Blogs on HPSC152

There are no specific blogs for HPSC152, but you can read our latest blog posts.
Omicron Variant Antibodies

Customers Who Bought This Also Bought

Contact Information

Download our Western Blot eHandbook

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our HPSC152 Antibody and receive a gift card or discount.


Bioinformatics

Gene Symbol TRMT112