HPRG Overexpression Lysate (Native) Summary
The lysate was created in HEK293T cells, using plasmid ID RC210166 and based on accession number NM_000412. The protein contains a C-terminal DDK tag.
Homo sapiens histidine-rich glycoprotein (HRG), mRNA.
Packaging, Storage & Formulations
Store at -80C. Avoid freeze-thaw cycles.
The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Lysate Details for HPRG
HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.
Alternate Names for HPRG Overexpression Lysate (Native)
- Histidine-proline-rich glycoprotein
- histidine-rich glycoprotein
This histidine-rich glycoprotein contains two cystatin-like domains and is located in plasma and platelets. The physiological function has not been determined but it is known that the protein binds heme, dyes and divalent metal ions. It can inhibit rosette formation and interacts with heparin, thrombospondin and plasminogen. Two of the protein's effects, the inhibition of fibrinolysis and the reduction of inhibition of coagulation, indicate a potential prothrombotic effect. Mutations in this gene lead to thrombophilia due to abnormal histidine-rich glycoprotein levels. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed
for 6 months from date of receipt.
Customers Who Viewed This Item Also Viewed...
Species: Hu, Ba
Applications: WB, ELISA
Applications: WB, Simple Western, ICC
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IP
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC, ICFlow
Species: Hu, Rt, Ca, Mk
Applications: WB, IHC, IHC-P, IP
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
Applications: WB, IHC
Applications: IHC, IHC-P
Publications for HPRG Lysate (NBL1-11707) (0)
There are no publications for HPRG Lysate (NBL1-11707).
By submitting your publication information earn gift cards and discounts for future purchases.
Reviews for HPRG Lysate (NBL1-11707) (0)
There are no reviews for HPRG Lysate (NBL1-11707).
By submitting a review earn points towards our Rewards Program.
- 250 points for product review
- 500 additional points for an image with your product review
- Double points (500) if you are the first to review this product
- Double points (1000) if you are the first to review this product with an image
Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
FAQs for HPRG Lysate (NBL1-11707) (0)
Additional HPRG Overexpression Lysate (Native) Products
Related Products by Gene
Bioinformatics Tool for HPRG Lysate (NBL1-11707)
Discover related pathways, diseases and genes to HPRG Lysate (NBL1-11707). Need help?
Read the Bioinformatics Tool Guide
for instructions on using this tool.
Blogs on HPRG