HP1 alpha Antibody [DyLight 650]

Images

 

Product Details

Summary
Product Discontinued
View other related HP1 alpha Primary Antibodies

Order Details


    • Catalog Number
      NB100-2518C
    • Availability
      Product Discontinued

    Can't find what you are looking for? Use our Antibody Concierge Service & we will help you locate your antibody!

    Or feel free to contact us for alternative products.

HP1 alpha Antibody [DyLight 650] Summary

Immunogen
A synthetic peptide containing an N-terminal region of human HP1 alpha (within residues 1-100). [Swiss-Prot P45973]
Localization
Nuclear
Marker
Heterochromatin Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CBX5
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence
  • Flow (Intracellular)
Application Notes
This HP1 alpha antibody is useful for Western blot analysis, where a band is seen at ~20 kDa.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human. Expected to react with mouse, Arabidopsis suecica, Rye and Triticum aestivum due to sequence homology.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
IgG purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for HP1 alpha Antibody [DyLight 650]

  • Antigen p25
  • chromobox homolog 5 (Drosophila HP1 alpha)
  • chromobox homolog 5 (HP1 alpha homolog, Drosophila)
  • chromobox homolog 5
  • chromobox protein homolog 5
  • Heterochromatin protein 1 homolog alpha
  • heterochromatin protein 1-alpha
  • HP1 alpha homolog
  • HP1 alpha
  • HP1
  • HP1A
  • HP1-ALPHA
  • HP1Hs alpha
  • HP1Hs-alpha

Background

Heterochromatin protein-1 (HP1) is a methyl-lysine binding protein localized at heterochromatin sites, where it mediates gene silencing. It has been shown that mammalian methyltransferases that selectively methylate histone H3 on lysine-9 generate a binding site for HP1 proteins, a family of heterochromatic adaptor molecules implicated in both gene silencing and supranucleosomal chromatin structure. High-affinity in vitro recognition of a methylated histone H3 peptide by HP1 requires a functional chromodomain. Thus, the HP1 chromodomain is a specific interaction motif for the methyl epitope on lysine-9 of histone H3. In vivo, heterochromatin association of HP1 proteins is lost in Suv39h double-null primary mouse fibroblasts but is restored after reintroduction of a catalytically active SUV39H1 HMTase. A molecular mechanism through which the SUV39H-HP1 methylation system can contribute to the propagation of heterochromatic subdomains in native chromatin has been defined. It has been demonstrated that HP1 can bind with high affinity to histone H3 methylated at lysine-9 but not at lysine-4. The chromodomain of HP1 as its methyl-lysine-binding domain has been identified. A point mutation in the chromodomain, which destroys the gene silencing activity of HP1 in Drosophila, abolished methyl-lysine-binding activity. Genetic and biochemical analysis in S. pombe showed that the methylase activity of Clr4 (the SUV39H1 homolog) is necessary for the correct localization of Swi6 (the HP1 equivalent) at centromeric heterochromatin and for gene silencing. A stepwise model for the formation of a transcriptionally silent heterochromatin: SUV39H1 places a methyl marker on histone H3, which is then recognized by HP1 through its chromodomain has been suggested. This model may also explain the stable inheritance of the heterochromatic state. SUV39H1 and HP1 are both involved in the repressive functions of the retinoblastoma protein. Rb associates with SUV39H1 and HP1 in vivo by means of its pocket domain. SUV39H1 cooperates with Rb to repress the cyclin E promoter, and in fibroblasts that are disrupted for SUV39H1, the activity of the cyclin E and cyclin A2 genes are specifically elevated. The SUV39H1-HP1 complex is not only involved in heterochromatic silencing but also has a role in repression of euchromatic genes by Rb and perhaps other corepressor proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB500-171
Species: Hu, Mu, Rt, Xp, Ye
Applications: WB, ChIP, ICC/IF, IHC, IHC-P
AF4409
Species: Mu
Applications: WB
NBP1-88824
Species: Hu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-17086
Species: Hu, Mu, Mk
Applications: WB, ICC/IF, IHC, IHC-P, IP
NBP2-14899
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NB100-2350
Species: Hu, Mu
Applications: WB, ICC/IF, IP, PLA
PP-A8620A-00
Species: Hu, Mu
Applications: WB, IHC, IP, DirELISA
NB100-56519
Species: Hu, Mu, Rt, Po
Applications: WB, Simple Western, ChIP, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, IHC-FrFl
NBP1-83228
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-14097
Species: Hu
Applications: IHC, IHC-P
NBP2-07996
Species: Hu
NBP2-52486
Species: Hu
Applications: WB, ELISA, Flow, IHC, IHC-P, CyTOF-ready, ICC, IF
H00144124-R02
Species: Hu
NBP1-33596
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP
NBP2-20322
Species: Hu
Applications: WB, ChIP, IP
NB600-41532
Species: Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NB100-2518C
Species: Hu
Applications: WB, Flow-IC, ICC/IF

Publications for HP1 alpha Antibody (NB100-2518C) (0)

There are no publications for HP1 alpha Antibody (NB100-2518C).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for HP1 alpha Antibody (NB100-2518C) (0)

There are no reviews for HP1 alpha Antibody (NB100-2518C). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for HP1 alpha Antibody (NB100-2518C) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional HP1 alpha Products

Bioinformatics Tool for HP1 alpha Antibody (NB100-2518C)

Discover related pathways, diseases and genes to HP1 alpha Antibody (NB100-2518C). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for HP1 alpha Antibody (NB100-2518C)

Discover more about diseases related to HP1 alpha Antibody (NB100-2518C).
 

Pathways for HP1 alpha Antibody (NB100-2518C)

View related products by pathway.

PTMs for HP1 alpha Antibody (NB100-2518C)

Learn more about PTMs related to HP1 alpha Antibody (NB100-2518C).
 

Research Areas for HP1 alpha Antibody (NB100-2518C)

Find related products by research area.

Blogs on HP1 alpha

There are no specific blogs for HP1 alpha, but you can read our latest blog posts.

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our HP1 alpha Antibody [DyLight 650] and receive a gift card or discount.

Bioinformatics

Gene Symbol CBX5