HOXD10 Antibody [Unconjugated]

HOXD10 was detected in immersion fixed human induced pluripotent stem (iPS) cells differentiated to neural progenitor cells (NPC) using Goat Anti-Human HOXD10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF10413) at 10 µg/mL, and co-stained with Mouse Anti-Human Nestin Monoclonal Antibody (Catalog # MAB1259) at 10 µg/mL, for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001), NorthernLights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green; Catalog # NL009) and counterstained with DAPI (blue). Specific staining for HOXD10 was localized to nucleus, while Nestin co-staining was cytoplasmic. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated

HOXD10 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human HOXD10
  Arg153-Thr262
  Accession # P28358
• Specificity: Detects human HOXD10 in direct ELISAs.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HOXD10 Antibody [Unconjugated]

• homeo box 4D
• homeo box D10
• homeobox D10
• Homeobox protein Hox-4D
• Homeobox protein Hox-4E
• homeobox protein Hox-D10
• HOX4
• Hox-4.4
• HOX4D
• HOX4DHOX4
• HOX4E
• HOXD10

Background

HOXD10 is a sequence-specific transcription factor that belongs to the Abd-B homeobox family.  It is a nuclear protein that is expressed in developing limb buds and is involved in differentiation and limb development.  HOXD10 mutations have been associated with foot deformities such as rocker-bottom foot and the deformity seen in Charcot-Marie-Tooth disease.  HOXD10 also functions as a candidate tumor suppressor.  In gastric cancer there is downregulation in gastric cancer tissues and cell lines relative to normal stomach tissues.  It has also been reported to suppress ovarian cancer, hepatocellular carcinoma and other cancer cells. Re-expression of HOXD10 results in significant inhibition of cell survival, induction of cell apoptosis and impairment of cell migration and invasion.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P28358()