HOXA11 Antibody

Images

 
Western Blot: HOXA11 Antibody [NBP1-80228] - Titration: 0.2-1 ug/ml, Positive Control: Human Uterus.
Immunohistochemistry-Paraffin: HOXA11 Antibody [NBP1-80228] - Human Uterus Tissue, 4-8ug/ml.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

HOXA11 Antibody Summary

Immunogen
Synthetic peptide directed towards the C terminal of human HOXA11. Peptide sequence KERRRRPESSSSPESSSGHTEDKAGGSGGQRTRKKRCPYTKYQIRELERE.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HOXA11
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
This is a rabbit polyclonal antibody against HOXA11 and was validated on Western blot.
Theoretical MW
34 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP1-80228 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS & 2% Sucrose.
Preservative
No Preservative
Purity
Immunogen affinity purified

Notes

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Alternate Names for HOXA11 Antibody

  • homeo box A11
  • homeobox A11
  • Homeobox protein Hox-1I
  • homeobox protein HOXA11
  • homeobox protein Hox-A11
  • HOX1
  • HOX1Ihomeo box 1I

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HOXA11 Antibody (NBP1-80228) (0)

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Review for HOXA11 Antibody (NBP1-80228) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-80228:
Filter by Applications
IHC-P
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin HOXA11 NBP1-80228
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reviewed by:
Ashwini Katre
IHC-P Human 03/05/2014
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedHuman pap serous carcinoma
SpeciesHuman
LotQC6280

Blocking

Blocking Details10% goat serum room temp 1 hour

Primary Anitbody

Dilution Ratio1:300 in 10% goat serum overnight at 4degrees

Secondary Antibody

Secondary Descriptiongoat anti rabbit HRP polymer from vector labs 1 hour RT
Secondary Manufacturer Cat#MP-7401
Secondary Concentrationready to use

Details

Detection NotesDAB for 3 min
Fixation DetailsAntigen retrieval in pH6 citrate buffer pressure cook for 5 min high pressure
Wash Description1XPBS 3minX3

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for HOXA11 Antibody (NBP1-80228) (0)

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Secondary Antibodies

 

Isotype Controls

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Diseases for HOXA11 Antibody (NBP1-80228)

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Ashwini Katre
03/05/2014
Application: IHC-P
Species: Human

Bioinformatics

Gene Symbol HOXA11
Uniprot