HOXA11 Antibody

Western Blot: HOXA11 Antibody [NBP1-80228] - Titration: 0.2-1 ug/ml, Positive Control: Human Uterus.

Immunohistochemistry-Paraffin: HOXA11 Antibody [NBP1-80228] - Human Uterus Tissue, 4-8ug/ml.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca, Eq, Rb
• Applications: WB, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

HOXA11 Antibody Summary

• Immunogen: Synthetic peptide directed towards the C terminal of human HOXA11. Peptide sequence KERRRRPESSSSPESSSGHTEDKAGGSGGQRTRKKRCPYTKYQIRELERE. The peptide sequence for this immunogen was taken from within the described region.
• Predicted Species: Rat (100%), Porcine (93%), Rabbit (93%), Bovine (93%), Canine (93%), Equine (93%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HOXA11
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
• Application Notes: This is a rabbit polyclonal antibody against HOXA11 and was validated on Western blot.
• Theoretical MW: 34 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 31665361).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS & 2% Sucrose.
• Preservative: 0.09% Sodium Azide
• Purity: Immunogen affinity purified

Notes

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Alternate Names for HOXA11 Antibody

• homeo box A11
• homeobox A11
• Homeobox protein Hox-1I
• homeobox protein HOXA11
• homeobox protein Hox-A11
• HOX1
• HOX1Ihomeo box 1I

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HOXA11 Antibody (NBP1-80228)(2)

Publications using NBP1-80228

• Grasso A, Navarro R, Balaguer N et al. Endometrial liquid biopsy provides a miRNA roadmap of the secretory phase of the human endometrium J. Clin. Endocrinol. Metab. Oct 29 2019 [PMID: 31665361] (Human)
• Tal R, Shaikh S, Pallavi P et al. Adult bone marrow progenitors become decidual cells and contribute to embryo implantation and pregnancy PLoS Biol. Sep 1 2019 [PMID: 31513564] (WB, Mouse)

Review for HOXA11 Antibody (NBP1-80228) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Ashwini Katre IHC-P Human 03/05/2014

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Human pap serous carcinoma
• Species: Human
• Lot: QC6280

Blocking

• Blocking Details: 10% goat serum room temp 1 hour

Primary Anitbody

• Dilution Ratio: 1:300 in 10% goat serum overnight at 4degrees

Secondary Antibody

• Secondary Description: goat anti rabbit HRP polymer from vector labs 1 hour RT
• Secondary Manufacturer Cat#: MP-7401
• Secondary Concentration: ready to use

Details

• Detection Notes: DAB for 3 min
• Fixation Details: Antigen retrieval in pH6 citrate buffer pressure cook for 5 min high pressure
• Wash Description: 1XPBS 3minX3

Bioinformatics

• Gene Symbol: HOXA11
• Uniprot:
  • NP_034580(Mouse)