HNRPGT Antibody (6F11) - Azide and BSA Free

Western Blot: HNRPGT Antibody (6F11) [H00027288-M01] - HNRNPG-T monoclonal antibody (M01), clone 6F11 Analysis of HNRNPG-T expression in HeLa.

Immunocytochemistry/Immunofluorescence: HNRPGT Antibody (6F11) [H00027288-M01] - Analysis of monoclonal antibody to HNRNPG-T on HeLa cell. Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: HNRPGT Antibody (6F11) [H00027288-M01] - Analysis of monoclonal antibody to HNRNPG-T on formalin-fixed paraffin-embedded human testis. Antibody concentration 3 ug/ml.

Sandwich ELISA: HNRPGT Antibody (6F11) [H00027288-M01] - Detection limit for recombinant GST tagged HNRNPG-T is approximately 0.1ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 6F11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

HNRPGT Antibody (6F11) - Azide and BSA Free Summary

• Immunogen: HNRNPG-T (NP_055284, 1 a.a. ~ 90 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MVEADRPGKLFIGGLNLETDEKALEAEFGKYGRIVEVLLMKDRETNKSRGFAFVTFESPADAKAAARDMNGKSLDGKAIKVAQATKPAFE
• Specificity: HNRNPG-T - testes-specific heterogenous nuclear ribonucleoprotein G-T
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: RBMXL2
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), and ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for HNRPGT Antibody (6F11) - Azide and BSA Free

• hnRNP G-T
• HNRNPG-T
• HNRNPGTRNA-binding motif protein, X-linked-like-2
• HNRPGTheterogeneous nuclear ribonucleoprotein G T
• RNA binding motif protein, X-linked-like 2
• testes specific heterogenous nuclear ribonucleoprotein G T
• testes-specific heterogenous nuclear ribonucleoprotein G-T
• Testis-specific heterogeneous nuclear ribonucleoprotein G-T

Background

This gene belongs to the HNRPG subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two RRM domains that bind RNAs. This gene is intronless and is thought to be derived from a processed retroposon. However, unlike many retroposon-derived genes, this gene is not a pseudogene. The encoded protein has similarity to HNRPG and RBMY proteins and it is suggested to replace HNRPG protein function during meiotic prophase or act as a germ cell-specific splicing regulator. It primarily localizes to the nuclei of meiotic spermatocytes. This gene is a candidate for autosomal male infertility.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RBMXL2
• Entrez:
  • 27288(Human)
• OMIM:
  • 605444(Human)
• Uniprot:
  • NP_055284(Human)