hnRNP-Q Antibody (JG35-72)

Western Blot: hnRNP-Q Antibody (JG35-72) [NBP2-75527] - Analysis of hnRNP Q on rat brain tissue lysates with Rabbit anti-hnRNP Q antibody at 1/500 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 70 kDa Observed band size: 70 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry/Immunofluorescence: hnRNP-Q Antibody (JG35-72) [NBP2-75527] - Staining hnRNP Q in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: hnRNP-Q Antibody (JG35-72) [NBP2-75527] - Analysis of paraffin-embedded human breast tissue with Rabbit anti-hnRNP-Q antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Western Blot: hnRNP-Q Antibody (JG35-72) [NBP2-75527] - Analysis of hnRNP Q on K562 cell lysate using anti-hnRNP Q antibody at 1/1,000 dilution.

Immunocytochemistry/Immunofluorescence: hnRNP-Q Antibody (JG35-72) [NBP2-75527] - Staining hnRNP Q in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: hnRNP-Q Antibody (JG35-72) [NBP2-75527] - Analysis of paraffin-embedded human thyroid gland tissue using anti-hnRNP Q antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: hnRNP-Q Antibody (JG35-72) [NBP2-75527] - Analysis of paraffin-embedded human breast tissue using anti-hnRNP Q antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: hnRNP-Q Antibody (JG35-72) [NBP2-75527] - Analysis of paraffin-embedded human kidney tissue using anti-hnRNP Q antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: hnRNP-Q Antibody (JG35-72) [NBP2-75527] - Analysis of paraffin-embedded rat testis tissue using anti-hnRNP Q antibody. Counter stained with hematoxylin.

Product Discontinued

hnRNP-Q Antibody (JG35-72) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human hnRNP-Q aa 574-623 / 623. (SwissProt: O60506 Human; SwissProt: Q7TP47 Rat; SwissProt: Q7TMK9 Mouse)
• Localization: Cytoplasm, Endoplasmic reticulum, Microsome, Nucleus, Spliceosome.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: SYNCRIP
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2000
• Theoretical MW: 70 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for hnRNP-Q Antibody (JG35-72)

• dJ3J17.2
• Glycine- and tyrosine-rich RNA-binding protein
• GRYRBP
• GRY-RBPNS1-associated protein 1
• heterogeneous nuclear ribonucleoprotein Q
• hnRNP Q
• hnRNP-Q
• HNRPQ
• HNRPQ1
• NSAP1FLJ31626
• PP68
• synaptotagmin binding, cytoplasmic RNA interacting protein
• Synaptotagmin-binding, cytoplasmic RNA-interacting protein

Background

Heterogenous nuclear ribonucleoprotein (hnRNP) implicated in mRNA processing mechanisms. Component of the CRD-mediated complex that promotes MYC mRNA stability. Isoform 1, isoform 2 and isoform 3 are associated in vitro with pre-mRNA, splicing intermediates and mature mRNA protein complexes. Isoform 1 binds to apoB mRNA AU-rich sequences. Isoform 1 is part of the APOB mRNA editosome complex and may modulate the postranscriptional C to U RNA-editing of the APOB mRNA through either by binding to A1CF (APOBEC1 complementation factor), to APOBEC1 or to RNA itself. May be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. Interacts in vitro preferentially with poly(A) and poly(U) RNA sequences. Isoform 3 may be involved in cytoplasmic vesicle-based mRNA transport through interaction with synaptotagmins

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SYNCRIP