hnRNP M1-M4 Antibody (1D8)

Images

 
Western Blot: hnRNP M1-M4 Antibody (1D8) [NB200-314] - Analysis of hnRNP M1-M4 in HeLa cell lysates using NB200-314.
Immunohistochemistry-Paraffin: hnRNP M1-M4 Antibody (1D8) [NB200-314] - Analysis of formalin fixed paraffin-embedded (FFPE) human tonsil using hnRNP M1-M4 (1D8) antibody at 1:500 on a Bond Rx autostainer (Leica ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, RbSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clone
1D8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

hnRNP M1-M4 Antibody (1D8) Summary

Immunogen
M19 fusion protein containing full-length human protein. [UniProt# P52272]
Specificity
NB200-314 is specific for all four M proteins of hnRNP (M1, M2, M3 and M4).
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
HNRNPM
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:500
Control
hnRNP M Overexpression Lysate
Reviewed Applications
Read 1 Review rated 1
using
NB200-314 in the following applications:

Publications
Read Publications using
NB200-314 in the following applications:

  • 1 publication
  • 1 publication
  • IP
    1 publication
  • PLA
    1 publication
  • WB
    6 publications

Reactivity Notes

A weak reactivity to mouse has been observed. Mouse reactivity reported in scientific literature (PMID:32931733)

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.1% Sodium Azide
Purity
Unpurified

Alternate Names for hnRNP M1-M4 Antibody (1D8)

  • DKFZp547H118
  • heterogeneous nuclear ribonucleoprotein M
  • hnRNP M
  • HNRNPM4
  • HNRPM4heterogenous nuclear ribonucleoprotein M
  • HNRPMheterogenous nuclear ribonucleoprotein M4
  • HTGR1
  • M4 protein
  • N-acetylglucosamine receptor 1
  • NAGR1hnRNA-binding protein M4

Background

RNA-binding proteins of mammalian hnRNP (heterogeneous nuclear ribonucleo protein) family represents a group of over 20 distinct evolutionary conserved proteins which share a capability to bind pre-mRNA/mRNA, and also binds to specific RNA motifs with high-affinity. They exhibit huge functional diversity and implicates in almost every step in mRNA's life cycle (from transcription and processing to transport and translation). HnRNP M is an abundant protein containing three RNA recognition motifs (RRM), a glycine/methionine-rich region and a methionine/arginine-rich repeat motif. It has four protein variants, hnRNP M1-4, ranging in molecular size from 68 to 72 kD which corresponds to spliced isoforms and/or posttranslationally modified forms. hnRNP M is a nucleolar protein and has been Identified in the spliceosome C complex. It acts as a pre-mRNA binding protein in vivo, binds avidly to poly(G) and poly(U) RNA homopolymers in vitro, and is involved in splicing. hnRNP M acts as a receptor for carcinoembryonic antigen in Kupffer cells, may initiate a series of signaling events leading to tyrosine phosphorylation of proteins and induction of IL-1 alpha, IL-6, IL-10 and TNF-alpha cytokines.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for hnRNP M1-M4 Antibody (NB200-314)(9)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Bovine.

We have publications tested in 5 applications: Func, ICC/IF, IP, PLA, WB.


Filter By Application
Func
(1)
ICC/IF
(1)
IP
(1)
PLA
(1)
WB
(6)
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Human
(4)
Mouse
(3)
Rat
(1)
Bovine
(1)
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Showing Publications 1 - 9 of 9.
Publications using NB200-314 Applications Species
Lleres D, Denegri M, Biggiogera M, Ajuh P, Lamond AI. Direct interaction between hnRNP-M and CDC5L/PLRG1 proteins affects alternative splice site choice. EMBO Rep;11(6):445-51. 2010 Jun. [PMID: 20467437] (WB, Human) WB Human
Vassileva MT, Matunis MJ. SUMO modification of heterogeneous nuclear ribonucleoproteins. Mol Cell Biol;24(9):3623-32. 2004 May. [PMID: 15082759] (IP, Human) IP Human
Kafasla P, Patrinou-Georgoula M, Guialis A. The 72/74-kDa polypeptides of the 70-110 S large heterogeneous nuclear ribonucleoprotein complex (LH-nRNP) represent a discrete subset of the hnRNP M protein family. Biochem J;350 Pt 2:495-503. 2000 Sep 1. [PMID: 10947964] (WB, Rat) WB Rat
Hehman GL, Hauswirth WW. DNA helicase from mammalian mitochondria. Proc Natl Acad Sci U S A;89(18):8562-6. 1992 Sep 15. [PMID: 1326759] (WB, Bovine) WB Bovine
Birladeanu A, Rogalska M, Potiri M, Papadaki V Nuclear IQGAP1 promotes gastric cancer cell growth by altering the splicing of a cell-cycle regulon in co-operation with hnRNPM bioRxiv Jan 1 2020 (Func, Mouse) Func Mouse
Birladeanu A, Rogalska M, Potiri M et al. The IQGAP1-hnRNPM interaction links tumour-promoting alternative splicing to heat-induced signals bioRxiv May 14 2020 (ICC/IF, PLA, Human) ICC/IF, PLA Human
Zhao Q, Liu J, Deng H et al. Targeting Mitochondria-Located circRNA SCAR Alleviates NASH via Reducing mROS Output Cell Sep 5 2020 [PMID: 32931733] (WB, Human, Mouse) WB Human, Mouse
Timchenko L T, Miller J W et al. Identification of a (CUG)n triplet repeat RNA-binding protein and its expression in myotonic dystrophy. Nucleic Acids Res Nov 15 1996 [PMID: 8948631] (WB, Mouse) WB Mouse
Birladeanu A, Rogalska M, Potiri M, Papadaki V The Scaffold Protein IQGAP1 Links Heat-Induced Stress Signals to Alternative Splicing Regulation in Gastric Cancer Cells SSRN Jan 1 2020 (WB) WB

Review for hnRNP M1-M4 Antibody (NB200-314) (1) 11

Average Rating: 1
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB200-314:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
  1
reviewed by:
Panagiota Kafasla
WB Mouse 07/09/2013
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Summary

ApplicationWestern Blot
Sample TestedMouse total lysate, macrophages
SpeciesMouse
LotA
CommentsIt does not seem to work for mouse extracts

Blocking

Blocking Details4% milk in TBS-T

Primary Anitbody

Dilution Ratio1:100 in TBS-T 4% milk, overnight 4 degrees

Secondary Antibody

Secondary Descriptionanti-mouse IgG1-HRP
Secondary Concentration1:2000

Details

Detection Notessouthern biotech

Comments

CommentsIt does not seem to work for mouse extracts

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for hnRNP M1-M4 Antibody (NB200-314) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

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1
1

 
Panagiota Kafasla
07/09/2013
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol HNRNPM
Entrez
Uniprot