hnRNP C1 + C2 Overexpression Lysate (Native)

Images

 
Western Blot: hnRNP C1 + C2 Overexpression Lysate (Adult Normal) [NBL1-11638] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for hnRNP C1 + C2.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

hnRNP C1 + C2 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC201695 and based on accession number NM_004500. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens heterogeneous nuclear ribonucleoprotein C (C1/C2) (HNRNPC), transcript variant 2, mRNA.
Gene
HNRNPC

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
32.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for hnRNP C1 + C2

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for hnRNP C1 + C2 Overexpression Lysate (Native)

  • C1
  • C2
  • heterogeneous nuclear ribonucleoprotein C (C1/C2)
  • heterogeneous nuclear ribonucleoproteins C1/C2
  • hnRNP C1/C2
  • HNRNP
  • hnRNPC
  • HNRPC
  • MGC104306
  • MGC105117
  • MGC117353
  • MGC131677
  • nuclear ribonucleoprotein particle C1 protein
  • nuclear ribonucleoprotein particle C2 protein
  • SNRPC

Background

This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene can act as a tetramer and is involved in the assembly of 40S hnRNP particles. Multiple transcript variants encoding at least two different isoforms have been described for this gene. Transcript Variant: This variant (2) lacks an alternate in-frame segment compared to variant 1. The resulting isoform (b, also known as isoform C1) has the same N- and C-termini but is shorter compared to isoform a. Variants 2 and 4 both encode isoform b. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB200-540
Species: Mu
Applications: WB, Flow, IA, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
NBP2-34234
Species: Hu
Applications: ICC/IF, IHC-Fr, IHC-P
NBP1-88927
Species: Hu
Applications: ICC/IF, IHC, IHC-P
H00005688-M01
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC-P, IP
NBP2-30078
Species: Hu
Applications: WB, Flow, ICC/IF, IHC-P
NB100-230
Species: Hu
Applications: WB, IP, PLA
NBP1-89342
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P
NBP2-45570
Species: Hu
Applications: WB, Flow, IHC
NB100-233
Species: Hu
Applications: WB, PLA
NBP1-90306
Species: Hu
Applications: WB, IHC, IHC-P
NBP1-59069
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NBL1-11228
Species: Hu
NBP1-43435
Species: Hu, Mu
Applications: WB, Flow
H00728695-B01P
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC-P
NBP1-83013
Species: Hu
Applications: WB, IHC, IHC-P
NBP1-32870
Species: Hu, Ch
Applications: WB, IHC, IHC-P, IP
NB100-63270
Species: Hu
Applications: WB, ELISA
H00000712-P01
Species: Hu
NBL1-11638
Species: Hu
Applications: WB

Publications for hnRNP C1 + C2 Lysate (NBL1-11638) (0)

There are no publications for hnRNP C1 + C2 Lysate (NBL1-11638).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for hnRNP C1 + C2 Lysate (NBL1-11638) (0)

There are no reviews for hnRNP C1 + C2 Lysate (NBL1-11638). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for hnRNP C1 + C2 Lysate (NBL1-11638) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional hnRNP C1 + C2 Products

Bioinformatics Tool for hnRNP C1 + C2 Lysate (NBL1-11638)

Discover related pathways, diseases and genes to hnRNP C1 + C2 Lysate (NBL1-11638). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for hnRNP C1 + C2 Lysate (NBL1-11638)

Discover more about diseases related to hnRNP C1 + C2 Lysate (NBL1-11638).
 

Pathways for hnRNP C1 + C2 Lysate (NBL1-11638)

View related products by pathway.

PTMs for hnRNP C1 + C2 Lysate (NBL1-11638)

Learn more about PTMs related to hnRNP C1 + C2 Lysate (NBL1-11638).
 

Research Areas for hnRNP C1 + C2 Lysate (NBL1-11638)

Find related products by research area.

Blogs on hnRNP C1 + C2

There are no specific blogs for hnRNP C1 + C2, but you can read our latest blog posts.

Customers Who Bought This Also Bought

CD20 Antibody (AISB12)
NBP1-43435-0.1mg

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our hnRNP C1 C2 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol HNRNPC