![Western Blot: hnRNP C1 + C2 Antibody (SN0652) [NBP2-67656] - Analysis of hnRNP C1+C2 on different lysates using anti-hnRNP C1+C2 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: MCF-7 Lane 3: HepG2](http://images.novusbio.com/fullsize2/hnRNP-C1-+-C2-Antibody-(SN0652)-Western-Blot-NBP2-67656-img0009.jpg "Western Blot: hnRNP C1 + C2 Antibody (SN0652) [NBP2-67656] - Analysis of hnRNP C1+C2 on different lysates using anti-hnRNP C1+C2 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: MCF-7 Lane 3: HepG2")
| Reactivity | Hu, Mu, Rt, ZeSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, IHC-P, IP |
| Clone | SN0652 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | Full length protein |
| Localization | Nucleus. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | HNRNPC |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | TBS (pH7.4), 1%BSA, 40%Glycerol |
| Preservative | 0.05% Sodium Azide |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Diseases for hnRNP C1 + C2 Antibody (NBP2-67656)Discover more about diseases related to hnRNP C1 + C2 Antibody (NBP2-67656).
| Pathways for hnRNP C1 + C2 Antibody (NBP2-67656)View related products by pathway.
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| Gene Symbol | HNRNPC |
![Immunocytochemistry/Immunofluorescence: hnRNP C1 + C2 Antibody (SN0652) [NBP2-67656] - Staining hnRNP C1+C2 in B16-F1 (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize2/hnRNP C1 + C2 Antibody (SN0652)-Immunocytochemistry-Immunofluorescence-NBP2-67656-img0010.jpg "Immunocytochemistry/Immunofluorescence: hnRNP C1 + C2 Antibody (SN0652) [NBP2-67656] - Staining hnRNP C1+C2 in B16-F1 (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: hnRNP C1 + C2 Antibody (SN0652) [NBP2-67656] - Analysis of paraffin-embedded mouse skin tissue using anti-hnRNP C1+C2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/hnRNP-C1-+-C2-Antibody-(SN0652)-Immunohistochemistry-Paraffin-NBP2-67656-img0008.jpg "Immunohistochemistry-Paraffin: hnRNP C1 + C2 Antibody (SN0652) [NBP2-67656] - Analysis of paraffin-embedded mouse skin tissue using anti-hnRNP C1+C2 antibody. Counter stained with hematoxylin.")
![Flow Cytometry: hnRNP C1 + C2 Antibody (SN0652) [NBP2-67656] - Analysis of Hela cells with hnRNP C1+C2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody](http://images.novusbio.com/fullsize2/hnRNP-C1-+-C2-Antibody-(SN0652)-Flow-Cytometry-NBP2-67656-img0001.jpg "Flow Cytometry: hnRNP C1 + C2 Antibody (SN0652) [NBP2-67656] - Analysis of Hela cells with hnRNP C1+C2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody")
![Immunocytochemistry/Immunofluorescence: hnRNP C1 + C2 Antibody (SN0652) [NBP2-67656] - Staining hnRNP C1+C2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize2/hnRNP-C1-+-C2-Antibody-(SN0652)-Immunocytochemistry-Immunofluorescence-NBP2-67656-img0002.jpg "Immunocytochemistry/Immunofluorescence: hnRNP C1 + C2 Antibody (SN0652) [NBP2-67656] - Staining hnRNP C1+C2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunocytochemistry/Immunofluorescence: hnRNP C1 + C2 Antibody (SN0652) [NBP2-67656] - Staining hnRNP C1+C2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize2/hnRNP-C1-+-C2-Antibody-(SN0652)-Immunocytochemistry-Immunofluorescence-NBP2-67656-img0003.jpg "Immunocytochemistry/Immunofluorescence: hnRNP C1 + C2 Antibody (SN0652) [NBP2-67656] - Staining hnRNP C1+C2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: hnRNP C1 + C2 Antibody (SN0652) [NBP2-67656] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-hnRNP C1+C2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/hnRNP-C1-+-C2-Antibody-(SN0652)-Immunohistochemistry-Paraffin-NBP2-67656-img0004.jpg "Immunohistochemistry-Paraffin: hnRNP C1 + C2 Antibody (SN0652) [NBP2-67656] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-hnRNP C1+C2 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: hnRNP C1 + C2 Antibody (SN0652) [NBP2-67656] - Analysis of paraffin-embedded human kidney tissue using anti-hnRNP C1+C2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/hnRNP-C1-+-C2-Antibody-(SN0652)-Immunohistochemistry-Paraffin-NBP2-67656-img0005.jpg "Immunohistochemistry-Paraffin: hnRNP C1 + C2 Antibody (SN0652) [NBP2-67656] - Analysis of paraffin-embedded human kidney tissue using anti-hnRNP C1+C2 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: hnRNP C1 + C2 Antibody (SN0652) [NBP2-67656] - Analysis of paraffin-embedded mouse brain tissue using anti-hnRNP C1+C2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/hnRNP-C1-+-C2-Antibody-(SN0652)-Immunohistochemistry-Paraffin-NBP2-67656-img0006.jpg "Immunohistochemistry-Paraffin: hnRNP C1 + C2 Antibody (SN0652) [NBP2-67656] - Analysis of paraffin-embedded mouse brain tissue using anti-hnRNP C1+C2 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: hnRNP C1 + C2 Antibody (SN0652) [NBP2-67656] - Analysis of paraffin-embedded mouse placenta tissue using anti-hnRNP C1+C2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/hnRNP-C1-+-C2-Antibody-(SN0652)-Immunohistochemistry-Paraffin-NBP2-67656-img0007.jpg "Immunohistochemistry-Paraffin: hnRNP C1 + C2 Antibody (SN0652) [NBP2-67656] - Analysis of paraffin-embedded mouse placenta tissue using anti-hnRNP C1+C2 antibody. Counter stained with hematoxylin.")
![Flow (Intracellular): Complement C3 Antibody (11H9) [NB200-540] - An intracellular stain was performed on RAW 246.7 cells with Complement C3 (11H9-3-2) antibody NB200-540 (blue) and a matched isotype control NBP2-31382 (orange). Cells were either treated with 3uM Monensin for 3 hours to block the secretion of Complement C3 (B) or grown in normal media (A). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) PE-conjugated secondary antibody (F0102B, R&D Systems).](http://images.novusbio.com/fullsize2/Complement-C3-Antibody-(11H9)-Flow-(Intracellular)-NB200-540-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Complement C3 Antibody (11H9) [NB200-540] - C3 protein fragments deposited on kidney cells of MPL-lpr mouse. Staining with antibody 11H9. Glomerular staining pattern. Fixation in 4% paraformaldehyde in PBS pH 7.4. Vibratome sections of 4 um. Pretreated with 3% hydrogen peroxide for 20 min to quench endogenous peroxidases. Microwaved in antigen unmasking solution for 2-5 minutes as antigen retrieval.](http://images.novusbio.com/fullsize2/Complement-C3-Antibody-(11H9)-Immunocytochemistry-Immunofluorescence-NB200-540-img0002.jpg)
![Immunohistochemistry-Paraffin: Complement C3 Antibody (11H9) [NB200-540] - Complement C3 protein in a FFPE tissue section of mouse liver using 1:100 dilution of Complement C3 antibody (clone 11H9) NB200-540. Weak but distinct membrane-cytoplasmic immunopositivity was observed in hepatocytes and few cells developed punctate membrane staining.](http://images.novusbio.com/fullsize2/Complement-C3-Antibody-(11H9)-Immunohistochemistry-Paraffin-NB200-540-img0004.jpg)
-img0001.jpg)
![Western Blot: BBS9 Antibody [NBP1-88927] - Analysis in human cell line RT-4 and human cell line U-251 MG.](http://images.novusbio.com/fullsize2/BBS9-Antibody-Western-Blot-NBP1-88927-img0004.jpg)
![Immunohistochemistry-Paraffin: BBS9 Antibody [NBP1-88927] - Staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells.](http://images.novusbio.com/fullsize2/BBS9-Antibody-Immunohistochemistry-Paraffin-NBP1-88927-img0002.jpg)
![Western Blot: PSMA7 Antibody (1A10-3G12) [H00005688-M01] - PSMA7 monoclonal antibody (M01), clone 1A10-3G12. Analysis of PSMA7 expression in human omentum, serous carcinoma.](http://images.novusbio.com/fullsize2/PSMA7-Antibody-(1A10-3G12)-Western-Blot-H00005688-M01-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: PSMA7 Antibody (1A10-3G12) [H00005688-M01] - Analysis of monoclonal antibody to PSMA7 on HeLa cell. Antibody concentration 1 ~ 10 ug/ml.](http://images.novusbio.com/fullsize2/PSMA7-Antibody-(1A10-3G12)-Immunocytochemistry-Immunofluorescence-H00005688-M01-img0009.jpg)
![Immunohistochemistry-Paraffin: PSMA7 Antibody (1A10-3G12) [H00005688-M01] - Analysis of monoclonal antibody to PSMA7 on formalin-fixed paraffin-embedded human colon tissue. Antibody concentration 5 ug/ml.](http://images.novusbio.com/fullsize2/PSMA7-Antibody-(1A10-3G12)-Immunohistochemistry-Paraffin-H00005688-M01-img0013.jpg)
![Western Blot: Complement C6 Antibody [NBP1-91803] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted)](http://images.novusbio.com/fullsize2/Complement C6 Antibody-Western Blot-NBP1-91803-img0004.jpg)
![Immunohistochemistry-Paraffin: Complement C6 Antibody [NBP1-91803] - Staining of human prostate shows strong granular cytoplasmic and membranous positivity in glandular cells.](http://images.novusbio.com/fullsize2/Complement-C6-Antibody-Immunohistochemistry-Paraffin-NBP1-91803-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: ATP6V0A1 Antibody [NBP1-89342] - Staining of human cell line A-431 shows localization to the Golgi apparatus & vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize2/ATP6V0A1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89342-img0006.jpg)
![Immunohistochemistry-Paraffin: ATP6V0A1 Antibody [NBP1-89342] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuropil.](http://images.novusbio.com/fullsize2/ATP6V0A1-Antibody-Immunohistochemistry-Paraffin-NBP1-89342-img0016.jpg)
![Immunohistochemistry-Paraffin: ATP6V0A1 Antibody [NBP1-89342] - Staining of human cerebral cortex shows high expression.](http://images.novusbio.com/fullsize2/ATP6V0A1-Antibody-Immunohistochemistry-Paraffin-NBP1-89342-img0010.jpg)
![Western Blot: RFC2 Antibody (2B2) [NBP2-45570] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RFC2.](http://images.novusbio.com/fullsize2/RFC2 Antibody (2B2)-Western Blot-NBP2-45570-img0013.jpg)
![Immunohistochemistry: RFC2 Antibody (2B2) [NBP2-45570] - Analysis of Adenocarcinoma of Human endometrium tissue.](http://images.novusbio.com/fullsize2/RFC2-Antibody-(2B2)-Immunohistochemistry-NBP2-45570-img0012.jpg)
![Flow Cytometry: RFC2 Antibody (2B2) [NBP2-45570] - Analysis of HEK293T cells transfected with either overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by RFC2 antibody.](http://images.novusbio.com/fullsize2/RFC2-Antibody-(2B2)-Flow-Cytometry-NBP2-45570-img0001.jpg)
![Western Blot: RFC4 Antibody (1A8) [NBP2-45946] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RFC4.](http://images.novusbio.com/fullsize2/RFC4-Antibody-(1A8)-Western-Blot-NBP2-45946-img0005.jpg)
![Immunohistochemistry: RFC4 Antibody (1A8) [NBP2-45946] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize2/RFC4-Antibody-(1A8)-Immunohistochemistry-NBP2-45946-img0004.jpg)
![Western Blot: RFC4 Antibody (1A8) [NBP2-45946] - Analysis of extracts (35ug) from 9 different cell lines by using RFC4 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize2/RFC4 Antibody (1A8)-Western Blot-NBP2-45946-img0006.jpg)
![Western Blot: alpha-Sarcoglycan Antibody (JA51-81) [NBP2-67150] - Analysis of alpha sarcoglycan on rat heart tissue lysates using anti-alpha sarcoglycan antibody at 1:500 dilution.](http://images.novusbio.com/fullsize2/alpha-Sarcoglycan Antibody (JA51-81)-Western Blot-NBP2-67150-img0003.jpg)
![Immunohistochemistry-Frozen: alpha-Sarcoglycan Antibody (JA51-81) [NBP2-67150] - Human skeletal muscle tissue. IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/fullsize2/alpha-Sarcoglycan-Antibody-(JA51-81)-Immunohistochemistry-Frozen-NBP2-67150-img0005.jpg)
![Immunohistochemistry-Paraffin: alpha-Sarcoglycan Antibody (JA51-81) [NBP2-67150] - Analysis of paraffin-embedded mouse heart tissue using anti-alpha sarcoglycan antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/alpha-Sarcoglycan-Antibody-(JA51-81)-Immunohistochemistry-Paraffin-NBP2-67150-img0001.jpg)
![Western Blot: ATP6V0A2 Antibody [NBP1-59069] - HeLa cells, concentration 3.3 ug/ml.](http://images.novusbio.com/fullsize2/ATP6V0A2-Antibody-Western-Blot-NBP1-59069-img0002.jpg)
![Immunohistochemistry: ATP6V0A2 Antibody [NBP1-59069] - Human kidney lysate tissue at an antibody concentration of 5.0 ug/ml using anti-ATP6V0A2 antibody](http://images.novusbio.com/fullsize2/ATP6V0A2-Antibody-Immunohistochemistry-NBP1-59069-img0008.jpg)
![Western Blot: ATP6V0A2 Antibody [NBP1-59069] - Hela tissue lysate at a concentration of 1ug/ml.](http://images.novusbio.com/fullsize2/ATP6V0A2-Antibody-Western-Blot-NBP1-59069-img0001.jpg)
![Western Blot: GPHA2 Overexpression Lysate (Adult Normal) [NBL1-11228] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GPHA2.](http://images.novusbio.com/fullsize/GPHA2 Overexpression Lysate (Adult Normal)-Western Blot-NBL1-11228-img0002.jpg)
![Western Blot: MS4A1/CD20 Antibody (AISB12) [NBP1-43435] - Immunoblot of Balb/c thymus (lane 1) and A20 (lane 2) cell lysates with Anti-Mouse CD20 Purified.](http://images.novusbio.com/fullsize2/MS4A1-CD20-Antibody-(AISB12)-Western-Blot-NBP1-43435-img0002.jpg)
![Flow Cytometry: MS4A1/CD20 Antibody (AISB12) [NBP1-43435] - Staining of BALB/c splenocytes with Anti-Mouse CD19 Alexa Fluor (R) 647 and 1.0 micrograms conjugated anti-Mouse CD20 Purified followed by Anti-Rat IgG PE. Quadrant lines represent Rat IgG2a isotype control staining levels and cells in the lymphocyte gate were used for analysis.](http://images.novusbio.com/fullsize2/MS4A1-CD20-Antibody-(AISB12)-Flow-Cytometry-NBP1-43435-img0004.jpg)
![Western Blot: SPANXB1 Antibody [H00728695-B01P] - Analysis of SPANXB1 expression in transfected 293T cell line by SPANXB1 polyclonal antibody. Lane 1: SPANXB1 transfected lysate(11.33 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize2/SPANXB1 Antibody-Western Blot-H00728695-B01P-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: SPANXB1 Antibody [H00728695-B01P] - Analysis of purified antibody to SPANXB1 on Hs 181.Tes cell. (antibody concentration 10 ug/ml)](http://images.novusbio.com/fullsize2/SPANXB1-Antibody-Immunocytochemistry-Immunofluorescence-H00728695-B01P-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: PLEKHM1 Antibody [NBP2-38449] - Staining of human cell line U-2 OS shows localization to nucleoli & vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize2/PLEKHM1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38449-img0003.jpg)
![Immunohistochemistry-Paraffin: PLEKHM1 Antibody [NBP2-38449] - Staining of human small intestine shows moderate cytoplasmic postivity in glandular cells.](http://images.novusbio.com/fullsize2/PLEKHM1-Antibody-Immunohistochemistry-Paraffin-NBP2-38449-img0008.jpg)
![Immunohistochemistry-Paraffin: PLEKHM1 Antibody [NBP2-38449] - Staining of human cerebral cortex shows moderate postivity in neuropil.](http://images.novusbio.com/fullsize2/PLEKHM1-Antibody-Immunohistochemistry-Paraffin-NBP2-38449-img0005.jpg)
![Western Blot: Exosome component 10 Antibody [NBP1-32870] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EXOSC10 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize2/Exosome-component-10-Antibody-Western-Blot-NBP1-32870-img0015.jpg)
![Immunohistochemistry-Paraffin: Exosome component 10 Antibody [NBP1-32870] - Paraffin-embedded Cal27 xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize2/Exosome-component-10-Antibody-Immunohistochemistry-Paraffin-NBP1-32870-img0010.jpg)
![Western Blot: Exosome component 10 Antibody [NBP1-32870] - Sample: 1000 ug 293T whole cell lysate/extract A. 30 ug 293T whole cell lysate/extract, B. Control with 2. 5 ug of preimmune rabbit IgG, C. Immunoprecipitation of EXOSC 10 protein by 2. 5 ug of EXOSC 10 antibody 7. 5% SDS-PAGE gel.](http://images.novusbio.com/fullsize2/Exosome-component-10-Antibody-Western-Blot-NBP1-32870-img0011.jpg)
![Western Blot: Complement C7 Antibody (15D1) [NBP2-80406] - Analysis of Complement Complement C7 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (NBP2-80406, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Positive control: Lane 1: HepG2 cell lysateLane 2: K562 cell lysateLane 3: HUVEC cell lysate](http://images.novusbio.com/fullsize2/Complement C7 Antibody (15D1)-Immunohistochemistry-Paraffin-NBP2-80406-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Complement C7 Antibody (15D1) [NBP2-80406] - Staining of C7 in SHG-44 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (EM1901-14, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).](http://images.novusbio.com/fullsize2/Complement-C7-Antibody-(15D1)-Immunocytochemistry-Immunofluorescence-NBP2-80406-img0004.jpg)
![Flow Cytometry: Complement C7 Antibody (15D1) [NBP2-80406] - Analysis of Complement C7 was done on HCT116 cells. The cells were fixed, permeabilized and stained with the primary antibody (1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes. Unlabeled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize2/Complement-C7-Antibody-(15D1)-Flow-Cytometry-NBP2-80406-img0001.jpg)
![Western Blot: C1q Antibody [NBP1-87492] - Analysis in control (vector only transfected HEK293T lysate) and C1QA over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize2/C1q-Antibody-Western-Blot-NBP1-87492-img0012.jpg)
![Immunohistochemistry-Paraffin: C1q Antibody [NBP1-87492] - Staining of human skeletal muscle shows no positivity in striated muscle fibers as expected.](http://images.novusbio.com/fullsize2/C1q-Antibody-Immunohistochemistry-Paraffin-NBP1-87492-img0010.jpg)
![Western Blot: C1q Antibody [NBP1-87492] - Analysis in human spleen tissue.](http://images.novusbio.com/fullsize2/C1q-Antibody-Western-Blot-NBP1-87492-img0006.jpg)
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody. Image using the Azide Free form of this antibody.](http://images.novusbio.com/images2/Rabbit-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0006.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody. Image using the Azide Free form of this antibody.")