hnRNP C1 + C2 Antibody (4F4) - BSA Free

Western Blot: hnRNP C1 + C2 Antibody (4F4) - BSA Free [NB600-585] - HeLa cells nuclear extract were separated on SDS-PAGE and probed with Monoclonal Anti-hnRNP-C1/C2 antibody produced in Mouse, Clone: 4F4. The antibody was developed using 1:1000 Anti-Mouse IgG (Fab specific)-Alkaline Phosphatase antibody produced in Goat.Lanes:1. 0.1 ug/mL antibody2. 0.2 ug/mL antibody3. 0.4 ug/mL antibody4. Negative Control

Product Discontinued

hnRNP C1 + C2 Antibody (4F4) - BSA Free Summary

• Immunogen: RNP eluted from oligo (dT) cellulose column
• Localization: Nucleus
• Specificity: This detects a band at approximately 41 and 43kDa.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: HNRNPC
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Immunoprecipitation 1:10-1:500
  • Microarray
  • Western Blot 1:100-1:2000
• Application Notes: In Western Blot a 41kDa (C1) and 43kDa 9C3) bands are seen. Not tested in other applications. *Optimal dilutions should be determined by the end user.

Reactivity Notes

Cross-reacts with Human, Chicken and Monkey. May also react with Hamster.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 10mM PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Concentration: 2.0 mg/ml
• Purity: Protein A or G purified

Alternate Names for hnRNP C1 + C2 Antibody (4F4) - BSA Free

• C1
• C2
• heterogeneous nuclear ribonucleoprotein C (C1/C2)
• heterogeneous nuclear ribonucleoproteins C1/C2
• hnRNP C1/C2
• HNRNP
• hnRNPC
• HNRPC
• MGC104306
• MGC105117
• MGC117353
• MGC131677
• nuclear ribonucleoprotein particle C1 protein
• nuclear ribonucleoprotein particle C2 protein
• SNRPC

Background

RNA polymerase II transcripts in the nucleus are in complex with several proteins called heterogeneous nuclear ribonucleoproteins (hnRNPs). These proteins are important in biological activities such as transcription, pre-mRNA processing, cytoplasmic mRNA translation, and turnover. hnRNPs can be isolated either by immunoprecipitation or by sucrose gradient fractionation of cell extracts. When this is performed, the hnRNPs are isolated (consisting of protein groups named A to U), and many of these protein groups consist of more than one isoform. The major steadystate proteins of the isolated hnRNP complex are the A1, A2, B1, B2, C1, and C2 with a range of molecular weight starting with 34 kDa up to 43 kDa. The hnRNP-C proteins have a single RNP motif RNA-binding domain (RBD) of 80 to 100 amino acid long. The hnRNP -C proteins preferentially bind to uridine-rich RNA sequences. Oligomerization of the protein through leucine rich regions in its C-terminal end is important for RNA binding.4 Although its physiological action is unknown, mutation of the hnRNPC gene causes a embryonic lethal phenotype.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for hnRNP C1 + C2 Antibody (NB600-585)(1)

Publication using NB600-585

• Halbert D, Domenyuk V, Spetzler D et al. Aptamers and uses thereof United States Patent Application US 9958448 B2 2018-01-01

Bioinformatics

• Gene Symbol: HNRNPC
• Entrez:
  • 3183(Human)