hnRNP A1 Antibody (JA39-21)

Western Blot: hnRNP A1 Antibody (JA39-21) [NBP2-66891] - Western blot analysis of hnRNP A1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HepG2 cell lysate Lane 2: PC-12 cell lysate

Immunocytochemistry/Immunofluorescence: hnRNP A1 Antibody (JA39-21) [NBP2-66891] - Staining hnRNP A1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: hnRNP A1 Antibody (JA39-21) [NBP2-66891] - Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-hnRNP A1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Flow Cytometry: hnRNP A1 Antibody (JA39-21) [NBP2-66891] - Analysis of Jurkat cells with hnRNP A1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

Western Blot: hnRNP A1 Antibody (JA39-21) [NBP2-66891] - Analysis of hnRNP A1 on HepG2 (1) and PC-12 (2) cell using anti-hnRNP A1 antibody at 1/1,000 dilution.

Immunocytochemistry/Immunofluorescence: hnRNP A1 Antibody (JA39-21) [NBP2-66891] - Staining hnRNP A1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: hnRNP A1 Antibody (JA39-21) [NBP2-66891] - Staining hnRNP A1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: hnRNP A1 Antibody (JA39-21) [NBP2-66891] - Analysis of paraffin-embedded rat testis tissue using anti-hnRNP A1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: hnRNP A1 Antibody (JA39-21) [NBP2-66891] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-hnRNP A1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: hnRNP A1 Antibody (JA39-21) [NBP2-66891] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-hnRNP A1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: hnRNP A1 Antibody (JA39-21) [NBP2-66891] - Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-hnRNP A1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: hnRNP A1 Antibody (JA39-21) [NBP2-66891] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-hnRNP A1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Product Discontinued

hnRNP A1 Antibody (JA39-21) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human hnRNP A1 aa 301-350 / 372. (SwissProt: P09651 Human; SwissProt: P49312 Mouse; SwissProt: P04256 Rat)
• Localization: Nucleus. Cytoplasm.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: HNRNPA1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for hnRNP A1 Antibody (JA39-21)

• Helix-destabilizing protein
• heterogeneous nuclear ribonucleoprotein A1
• heterogeneous nuclear ribonucleoprotein A1B protein
• heterogeneous nuclear ribonucleoprotein B2 protein
• heterogeneous nuclear ribonucleoprotein core protein A1
• hnRNP A1
• hnRNP core protein A1
• hnRNPA1
• hnRNP-A1
• HNRPA1MGC102835
• nuclear ribonucleoprotein particle A1 protein
• single-strand DNA-binding protein UP1
• Single-strand RNA-binding protein

Background

RNA polymerase II transcripts in the nucleus are in complex with several proteins called heterogeneous nuclear ribonucleoproteins (hnRNPs). These proteins are important in biological activities such as transcription, pre-mRNA processing, cytoplasmic mRNA translation and turnover. hnRNPs can be isolated either by immunoprecipitation or by sucrose gradient fractionation of cell extracts.1, 3, 4 Isolated hnRNPs consist of protein groups named A to U and many of these protein groups consist of more than one isoform. The major steady-state proteins of the isolated hnRNP complex are A1, A2, B1, B2, C1, and C2, with a range of molecular weight starting with 34 kDa up to 43 kDa.1, 3, 4 hnRNP-A1 is important in pre-mRNA processing and in mRNA export from the nucleus. The protein binds to its RNA target through a consensus RNA-binding site UAGGGU. The protein contains a 38-amino acid domain called M9, which is important for the interaction with the transportin protein, and therefore, for its import and export from the nucleus. RanGTP mediates dissociation of hnRNP-A1 from transportin. hnRNP-A1 is ubiquitously expressed with a higher expression in proliferating and/or transformed cells than in differentiated tissues.5

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HNRNPA1