HNF-4 gamma/NR2A2 Antibody (N3224) [Unconjugated]

HNF‑4 gamma /NR2A2 was detected in perfusion fixed paraffin-embedded sections of rat small intestine using HNF‑4 gamma /NR2A2 Monoclonal Antibody (Catalog # PP‑N3224‑00) overnight at 4 °C. Tissue was stained (brown) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

HNF4G promotes resistance to cisplatin in lung adenocarcinoma cells.(A) qRT-PCR and (B) western blot results of HNF4G expression after cisplatin treatment in A549 and HCC827 cells. (C) qRT-PCR results of HNF4G expression in HNF4G overexpression and knockdown cells. (D) Cisplatin sensitivity curves of HNF4G overexpression and knockdown cells. * p < 0.05, ** p < 0.01, *** p < 0.001. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36923501), licensed under a CC-BY license. Not internally tested by R&D Systems.

HNF4G promotes resistance to cisplatin in lung adenocarcinoma cells.(A) qRT-PCR and (B) western blot results of HNF4G expression after cisplatin treatment in A549 and HCC827 cells. (C) qRT-PCR results of HNF4G expression in HNF4G overexpression and knockdown cells. (D) Cisplatin sensitivity curves of HNF4G overexpression and knockdown cells. * p < 0.05, ** p < 0.01, *** p < 0.001. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36923501), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: N3224
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

HNF-4 gamma/NR2A2 Antibody (N3224) [Unconjugated] Summary

• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: HNF4G

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
• Buffer: A liquid formulation in physiologic saline with 0.1% NaN₃.
• Preservative: 0.1% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HNF-4 gamma/NR2A2 Antibody (N3224) [Unconjugated]

• hepatocyte nuclear factor 4, gamma
• hepatocyte nuclear factor 4-gamma
• HNF4 gamma
• HNF-4 gamma
• HNF4G
• HNF-4-gamma
• NR2A2
• NR2A2Nuclear receptor subfamily 2 group A member 2
• NR2A3

Background

Hepatocyte Nuclear Factor 4 alpha (HNF4 alpha; NR2A1) is a member of the orphan nuclear receptor family. HNF4 alpha is expressed in the liver, kidney, intestine and pancreas. Mutation of HNF4 alpha in humans has been associated with maturity-onset diabetes of the young type 1 (MODY1). HNF4 binds to DNA as an exclusive homodimer. The HNF4 alpha gene is alternatively spliced and may generate up to nine different isoforms, HNF4 alpha 1 through HNF4 alpha 9. The expression of HNF-4 gamma (NR2A2) is observed in the pancreas, kidney, small intestine, and testes with no expression detected in the liver. The level of HNF-4 gamma is less 10% than that of HNF-4 alpha.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HNF-4 gamma/NR2A2 Antibody (PP-N3224-00)(1)

Publication using PP-N3224-00

• J Liang, G Zhao, Y Bian, G Bi, Q Sui, H Zhang, H Shi, G Shan, Y Huang, Z Chen, L Wang, C Zhan HNF4G increases cisplatin resistance in lung adenocarcinoma via the MAPK6/Akt pathway PeerJ, 2023-03-10;11(0):e14996. 3/10/2023 [PMID: 36923501]

Bioinformatics

• Gene Symbol: HNF4G