Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." alt="HNF‑4 gamma /NR2A2 was detected in perfusion fixed paraffin-embedded sections of rat small intestine using HNF‑4 gamma /NR2A2 Monoclonal Antibody (Catalog # PP‑N3224‑00) overnight at 4 °C. Tissue was stained (brown) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-82531-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-98899-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98898-50ul | |||
![Immunohistochemistry-Paraffin: HNF-4 gamma/NR2A2 Antibody [NBP1-82531] - Staining of human small intestine shows high expression.](http://images.novusbio.com/fullsize/HNF-4-gamma-NR2A2-Antibody-Immunohistochemistry-Paraffin-NBP1-82531-img0006.jpg "Immunohistochemistry-Paraffin: HNF-4 gamma/NR2A2 Antibody [NBP1-82531] - Staining of human small intestine shows high expression.")
![Immunohistochemistry-Paraffin: HNF-4 gamma/NR2A2 Antibody [NBP1-82531] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/fullsize/HNF-4-gamma-NR2A2-Antibody-Immunohistochemistry-Paraffin-NBP1-82531-img0005.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: HNF-4 gamma/NR2A2 Antibody [NBP1-82531] - Staining in human small intestine and liver tissues using anti-HNF4G antibody. Corresponding HNF4G RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/HNF-4-gamma-NR2A2-Antibody-Immunohistochemistry-Paraffin-NBP1-82531-img0007.jpg)
![Immunohistochemistry-Paraffin: HNF-4 gamma/NR2A2 Antibody [NLS5162] - Anti-HNF4G / HNF4 Gamma antibody IHC of human Pancreas, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/HNF-4-gamma-NR2A2-Antibody-Immunohistochemistry-Paraffin-NLS5162-img0006.jpg "Immunohistochemistry-Paraffin: HNF-4 gamma/NR2A2 Antibody [NLS5162] - Anti-HNF4G / HNF4 Gamma antibody IHC of human Pancreas, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.")
![Immunohistochemistry-Paraffin: HNF-4 gamma/NR2A2 Antibody [NLS5162] - Analysis of anti-HNF4G / HNF4 Gamma antibody with pancreas, Islet of Langerhans.](http://images.novusbio.com/fullsize/HNF-4-gamma-NR2A2-Antibody-Immunohistochemistry-Paraffin-NLS5162-img0003.jpg)
![Immunohistochemistry-Paraffin: HNF-4 gamma/NR2A2 Antibody [NLS5162] - Analysis of anti-HNF4G / HNF4 Gamma antibody with human breast, carcinoma.](http://images.novusbio.com/fullsize/HNF-4-gamma-NR2A2-Antibody-Immunohistochemistry-Paraffin-NLS5162-img0005.jpg)
![Immunohistochemistry-Paraffin: HNF-4 gamma/NR2A2 Antibody [NLS5162] - Anti-HNF4G antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/HNF-4-gamma-NR2A2-Antibody-Immunohistochemistry-Paraffin-NLS5162-img0007.jpg)
![Western Blot: HNF-4 gamma/NR2A2 Antibody [NBP1-52810] - Human Adult Placenta Antibody Dilution: 1.0 ug/ml.](http://images.novusbio.com/fullsize/HNF-4-gamma-NR2A2-Antibody-Western-Blot-NBP1-52810-img0003.jpg "Western Blot: HNF-4 gamma/NR2A2 Antibody [NBP1-52810] - Human Adult Placenta Antibody Dilution: 1.0 ug/ml.")
![Western Blot: HNF-4 gamma/NR2A2 Antibody [NBP1-52810] - MCF-7 whole cell lysates, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/HNF-4-gamma-NR2A2-Antibody-Western-Blot-NBP1-52810-img0002.jpg)
![Western Blot: HNF-4 gamma/NR2A2 Antibody [NBP2-98899] - Anti-HNF-4 gamma/NR2A2 rabbit polyclonal antibody at 1:500 dilution. Lane A: Mouse stomach tissue Lysate. Lane B: Mouse spleen tissue lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 46 kDa. Observed band size: 45 kDa](http://images.novusbio.com/fullsize/HNF-4-gamma-NR2A2-Antibody-Western-Blot-NBP2-98899-img0002.jpg "Western Blot: HNF-4 gamma/NR2A2 Antibody [NBP2-98899] - Anti-HNF-4 gamma/NR2A2 rabbit polyclonal antibody at 1:500 dilution. Lane A: Mouse stomach tissue Lysate. Lane B: Mouse spleen tissue lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 46 kDa. Observed band size: 45 kDa")
![Immunoprecipitation: HNF-4 gamma/NR2A2 Antibody [NBP2-98899] - HNF-4 gamma/NR2A2 was immunoprecipitated using: Lane A: 0.5 mg mouse spleen tissue Lysate 4 ul anti-HNF-4 gamma/NR2A2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads. Protein A/G. Primary antibody: Anti-HNF-4 gamma/NR2A2 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 46 kDa. Observed band size: 46 kDa](http://images.novusbio.com/fullsize/HNF-4-gamma-NR2A2-Antibody-Immunoprecipitation-NBP2-98899-img0003.jpg)
![Western Blot: HNF-4 gamma/NR2A2 Antibody [NBP2-98898] - Lane A: Mouse spleen tissue lysate Lysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:46 kDaObserved band size:46 kDa](http://images.novusbio.com/fullsize/HNF-4-gamma-NR2A2-Antibody-Western-Blot-NBP2-98898-img0001.jpg "Western Blot: HNF-4 gamma/NR2A2 Antibody [NBP2-98898] - Lane A: Mouse spleen tissue lysate Lysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:46 kDaObserved band size:46 kDa")
