HMGN1/HMG14 [p Ser20, p Ser24] Antibody

Images

 
Immunohistochemistry-Paraffin: HMG14 Antibody [NBP1-02428] - Staining of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ca, Ch, XpSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

HMGN1/HMG14 [p Ser20, p Ser24] Antibody Summary

Immunogen
Synthetic peptide from human HMGN1.
Modification
p Ser20, p Ser24
Specificity
Amino acids 19-28 of human HMGN protein containing pSer20 and pSer24
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HMGN1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500 - 1:2000
  • ELISA 1:5000 - 1:25000
  • Immunocytochemistry/Immunofluorescence 1:5000 - 1:25000
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 20 ug/ml

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20mM Potassium Phosphate (pH 7.2) and 0.15M NaCl
Preservative
0.01% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for HMGN1/HMG14 [p Ser20, p Ser24] Antibody

  • FLJ31471
  • High mobility group nucleosome-binding domain-containing protein 1
  • high-mobility group (nonhistone chromosomal) protein 14
  • high-mobility group nucleosome binding 1
  • high-mobility group nucleosome binding domain 1
  • HMG14
  • HMG14FLJ27265
  • HMGN1
  • MGC104230
  • MGC117425
  • non-histone chromosomal protein HMG-14
  • nonhistone chromosomal protein HMG-14

Background

HMGNs are proteins that bind chromatin effectively reducing the compaction of the chromatin fiber and enhancing access to DNA regulatory sequences. Members of this family have a conserved chromatin binding domain which is phosphorylated during mitosis. The sequence immunized is conserved in several species. As such, this reagent is designed as a universal reagent for the detection of all phosphorylated HMGN proteins. The High Mobility Group (HMG) proteins were originally isolated from mammalian cells and were named according to their electrophoretic mobility in polyacrylamide gels. HMGs were arbitrarily classed as a specific type of nonhistone proteins based on the observation that they are ubiquitous to mammalian cells, that they share certain physical properties, and that they are associated with isolated chromatin. HMG proteins and are now subdivided into 3 families: the HMGB (formerly HMG-1/-2) family, the HMGN (formerly HMG-14/-17) family, and the HMGA (formerly HMG-I/Y/C) family. Each HMG family has a characteristic functional sequence motif. The functional motif of the HMGB family is called the "HMG-box;" that of the HMGN family, the "nucleosomal binding domain;" and that of the HMGA family, the "AT-hook." The functional motifs characteristic of these canonical HMGs are widespread among nuclear proteins in a variety of organisms. Proteins containing any of these functional motifs embedded in their sequence are known as "HMG motif proteins."

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol HMGN1
Entrez
Uniprot