HMGN1/HMG14 [p Ser20, p Ser24] Antibody

Immunohistochemistry-Paraffin: HMG14 Antibody [NBP1-02428] - Staining of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.

Product Discontinued

HMGN1/HMG14 [p Ser20, p Ser24] Antibody Summary

• Immunogen: Synthetic peptide from human HMGN1/HMG14 corresponding to aa 19-28.
• Modification: p Ser20, p Ser24
• Specificity: Reactivity occurs against human HMGN pS20/pS24 proteins and the antibody is specific for the phosphorylated form of the proteins. Reactivity is seen against HMGN1 and HMGN2. HMGN3 is also likely reactive although not tested. Reactivity with non-phosphorylated human HMGNs is minimal by ELISA.
• Predicted Species: Mouse (100%), Rat (100%), Porcine (100%), Bovine (100%), Orangutan (100%), Chicken (100%), Canine (100%), Xenopus (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HMGN1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:5000 - 1:25000
  • Immunocytochemistry/ Immunofluorescence 1:5000 - 1:25000
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 20 ug/ml
  • Western Blot 1:500 - 1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20mM Potassium Phosphate (pH 7.2) and 0.15M NaCl
• Preservative: 0.01% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for HMGN1/HMG14 [p Ser20, p Ser24] Antibody

• FLJ31471
• High mobility group nucleosome-binding domain-containing protein 1
• high-mobility group (nonhistone chromosomal) protein 14
• high-mobility group nucleosome binding 1
• high-mobility group nucleosome binding domain 1
• HMG14
• HMG14FLJ27265
• HMGN1
• MGC104230
• MGC117425
• non-histone chromosomal protein HMG-14
• nonhistone chromosomal protein HMG-14

Background

HMGNs are proteins that bind chromatin effectively reducing the compaction of the chromatin fiber and enhancing access to DNA regulatory sequences. Members of this family have a conserved chromatin binding domain which is phosphorylated during mitosis. The sequence immunized is conserved in several species. As such, this reagent is designed as a universal reagent for the detection of all phosphorylated HMGN proteins. The High Mobility Group (HMG) proteins were originally isolated from mammalian cells and were named according to their electrophoretic mobility in polyacrylamide gels. HMGs were arbitrarily classed as a specific type of nonhistone proteins based on the observation that they are ubiquitous to mammalian cells, that they share certain physical properties, and that they are associated with isolated chromatin. HMG proteins and are now subdivided into 3 families: the HMGB (formerly HMG-1/-2) family, the HMGN (formerly HMG-14/-17) family, and the HMGA (formerly HMG-I/Y/C) family. Each HMG family has a characteristic functional sequence motif. The functional motif of the HMGB family is called the "HMG-box;" that of the HMGN family, the "nucleosomal binding domain;" and that of the HMGA family, the "AT-hook." The functional motifs characteristic of these canonical HMGs are widespread among nuclear proteins in a variety of organisms. Proteins containing any of these functional motifs embedded in their sequence are known as "HMG motif proteins."

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HMGN1
• Entrez:
  • 3150(Human)
  • 15312(Mouse)
  • 360704(Rat)
• Uniprot:
  • P05114()