HMGB3/HMG4 Antibody

Product Discontinued

HMGB3/HMG4 Antibody Summary

• Immunogen: Peptide with sequence C-KFDGAKGPAKVARKK corresponding to internal region (near C-Terminus) according to NP_005333.2.
• Epitope: internal region (near C Terminus)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: HMGB3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 0.01 - 0.03 ug/ml
• Application Notes: WB: Approx. 24 kDa band observed in human lung lysates (calculated MW of 23.0 kDa band according to NP_005333.2).

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Bovine.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris (pH 7.3), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for HMGB3/HMG4 Antibody

• High mobility group protein 2a
• High mobility group protein 4
• high mobility group protein B3
• high-mobility group (nonhistone chromosomal) protein 4
• high-mobility group box 3
• HMG2A
• HMG-2a
• HMG2AHMG-4
• HMG4
• HMG4MGC90319
• HMGB3
• non-histone chromosomal protein

Background

HMGB3 belongs to the high mobility group (HMG) protein superfamily. Like HMG1 (MIM 163905) and HMG2 (MIM 163906), HMGB3 contains DNA-binding HMG box domains and is classified into the HMG box subfamily. Members of the HMG box subfamily are thought to play a fundamental role in DNA replication, nucleosome assembly and transcription (Wilke et al., 1997 [PubMed 9370291]; Nemeth et al., 2006 [PubMed 16945912]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HMGB3/HMG4 Antibody (NBP1-26398)(2)

Publications using NBP1-26398

• Wang LL, Meng QH, Jiao Y et al. High-Mobility Group Boxes Mediate Cell Proliferation and Radiosensitivity via Retinoblastoma-Interaction-Dependent and -Independent Mechanisms Cancer Biother Radiopharm 2012-06-01 [PMID: 22655796]
• Yu F, Yao H, Zhu P et al. let-7 regulates self renewal and tumorigenicity of breast cancer cells. Cell 2007-12-14 [PMID: 18083101]

Bioinformatics

• Gene Symbol: HMGB3
• Entrez:
  • 3149(Human)
  • 15354(Mouse)
• Uniprot:
  • NP_005333.2(Human)