HMGB2 Antibody - BSA Free

Western Blot: HMGB2 Antibody [NBP2-38694] - Analysis in human cell lines U2OS and A-549. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-COX4I1.

Immunocytochemistry/Immunofluorescence: HMGB2 Antibody [NBP2-38694] - Staining of human cell line U-2 OS shows localization to nucleus and nucleoli. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: HMGB2 Antibody [NBP2-38694] - Staining of human skeletal muscle shows no positivity in myocytes as expected.

Immunohistochemistry-Paraffin: HMGB2 Antibody [NBP2-38694] - Analysis in human bone marrow and skeletal muscle tissues using NBP2-38694 antibody. Corresponding HMGB2 RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: HMGB2 Antibody [NBP2-38694] - Staining of human bone marrow shows strong nuclear positivity in hematopoietic cells.

Immunohistochemistry-Paraffin: HMGB2 Antibody [NBP2-38694] - Staining of human lymph node shows strong nuclear positivity in germinal center cells.

Immunohistochemistry-Paraffin: HMGB2 Antibody [NBP2-38694] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.

Immunohistochemistry-Paraffin: HMGB2 Antibody [NBP2-38694] - Staining of human colon shows strong nuclear positivity in glandular cells.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

HMGB2 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against a recombinant protein corresponding to amino acids: AKGKSEAGKKGPGRPTGSKKKNEP
• Predicted Species: Mouse (100%), Rat (96%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HMGB2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04 - 0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  HMGB2 Protein (NBP2-38694PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for HMGB2 Antibody - BSA Free

• High mobility group protein 2
• high mobility group protein B2
• high-mobility group box 2
• HMG-2
• HMG2high-mobility group (nonhistone chromosomal) protein 2

Background

HMGB2 encodes a member of the non-histone chromosomal high mobility group protein family. The proteins of this family are chromatin-associated and ubiquitously distributed in the nucleus of higher eukaryotic cells. In vitro studies have demonstrated that this protein is able to efficiently bend DNA and form DNA circles. These studies suggest a role in facilitating cooperative interactions between cis-acting proteins by promoting DNA flexibility. This protein was also reported to be involved in the final ligation step in DNA end-joining processes of DNA double-strand breaks repair and V(D)J recombination.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HMGB2
• Uniprot:
  • P26583(Human)