HMGA1 Antibody

Western Blot: HMGA1 Antibody [NB100-56468] - Analysis of HMGA1 in the 1) absence and 2) presence of immunizing peptide in human pancreas lysate using this antibody at 1 ug/ml.

Immunohistochemistry-Paraffin: HMGA1 Antibody [NB100-56468] - Staining of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

Product Discontinued

HMGA1 Antibody Summary

• Immunogen: A synthetic peptide (DGTEKRGRGRPRKQ) corresponding to amino acids 19-32 of human HMGA1 was used as immunogen.
• Predicted Species: Bovine (92%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HMGA1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1-3 ug/ml
• Control:

  Human Pancreas Whole Tissue Lysate (Adult Whole Normal)
  HMGA1 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS containing 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for HMGA1 Antibody

• high mobility group AT-hook 1
• High mobility group AT-hook protein 1
• High mobility group protein A1
• high mobility group protein HMG-I/HMG-Y
• High mobility group protein R
• high-mobility group (nonhistone chromosomal) protein isoforms I and Y
• HMGA1A
• HMG-I(Y)
• HMGIYMGC12816
• HMG-R
• MGC4242
• MGC4854
• nonhistone chromosomal high-mobility group protein HMG-I/HMG-Y

Background

DNA-binding non-histone nuclear protein is a key member of HMGA protein family. It consists of a highly conserved AT-hook DNA-binding domain that mediates binding to AT-rich sequences in the minor groove of chromosomal DNA. It functions as architectural chromatin-binding transcription factor altering the conformation of DNA by modulating nuclear protein-DNA complexes. It is involved in many cellular processes including growth regulation, viral induction of beta-IFN gene and regulation of inducible gene transcription. It also physically interacts with transcription factors such as AP-1, Sp-1 and CAAT/enhancer binding protein-beta to modulate their ability to activate gene expression. It functions as a direct transcriptional target of c-Jun and c-Myc and is maximally expressed during embryonic development. Pathological role of HMGA1 include integration of retroviruses into chromosomes and enhancing metastasis of tumor cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HMGA1
• Entrez:
  • 3159(Human)
  • 15361(Mouse)
  • 117062(Rat)
• Uniprot:
  • NP_665908(Human)