HMBS Antibody (SC65-07)

Western Blot: HMBS Antibody (SC65-07) [NBP2-67637] - Analysis of HMBS on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: 293T cell lysate

Western Blot: HMBS Antibody (SC65-07) [NBP2-67637] - Analysis of HMBS on different lysates using anti-HMBS antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: 293T

Western Blot: HMBS Antibody (SC65-07) [NBP2-67637] - Analysis of HMBS on K562 cell lysates with Rabbit anti-HMBS antibody at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 39 kDa Observed band size: 41 kDa Exposure time: 30 seconds; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.

Product Discontinued

HMBS Antibody (SC65-07) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human HMBS aa 1-132 / 299. (SwissProt: P08397 Human; SwissProt: P22907 Mouse; SwissProt: P19356 Rat)
• Localization: Cytoplasm.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: HMBS
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for HMBS Antibody (SC65-07)

• hydroxymethylbilane synthasePre-uroporphyrinogen synthase
• PBGDPBG-D
• PORC
• porphobilinogen deaminase
• porphyria, acute; Chester type
• UPSEC 2.5.1.61
• uroporphyrinogen I synthase
• uroporphyrinogen I synthetase

Background

HMBS encodes a member of the hydroxymethylbilane synthase superfamily. The encoded protein is the third enzyme of the heme biosynthetic pathway and catalyzes the head to tail condensation of four porphobilinogen molecules into the linear hydroxymethylbilane. Mutations in this gene are associated with the autosomal dominant disease acute intermittent porphyria. Alternatively spliced transcript variants encoding different isoforms have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HMBS