HLA F Antibody (3D11) [PE/Atto594]

• Reactivity: Hu
• Applications: Flow
• Clone: 3D11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: PE/Atto594

HLA F Antibody (3D11) [PE/Atto594] Summary

• Immunogen: Inclusion body-derived HLA-F heavy chain
• Specificity: Recognizes HLA-F, a 42 kDa type I transbembrane protein expressed on B cells, NK cells, monocytes, and T cells, but mainly in the endoplasmic reticulum and Golgi apparatus, only a small amount on the cell surface, where, however, it can be expressed after cell activation.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: HLA-F
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
• Application Notes: Optimal dilution of this antibody should be experimentally determined. For optimal results using our Tandem dyes, please avoid prolonged exposure to light or extreme temperature fluctuations. These can lead to irreversible degradation or decoupling. When staining intracellular targets, specific attention to the fixation and permeabilization steps in your flow protocol may be required. Please contact our technical support team at technical@novusbio.com if you have any questions.

Reactivity Notes

Recognizes HLA-F, a 42 kDa type I transbembrane protein expressed on B cells, NK cells, monocytes, and T cells, but mainly in the endoplasmic reticulum and Golgi apparatus, only a small amount on the cell surface, where, however, it can be expressed after cell activation.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein A purified

Alternate Names for HLA F Antibody (3D11) [PE/Atto594]

• alpha chain F
• CDA12
• HLA class I molecule
• HLA F antigen
• HLA-5.4
• HLAF
• HLA-F
• HLAFHLA-CDA12
• Leukocyte antigen F
• major histocompatibility complex, class I, F
• MHC class I antigen F
• MHC class Ib antigen

Background

The HLA F gene belongs to the HLA class I heavy chain paralogues. It encodes a non-classical heavy chain that forms a heterodimer with a beta-2 microglobulin light chain, with the heavy chain anchored in the membrane. Unlike most other HLA heavy chains, this m

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HLA-F