HLA DR/DP Antibody (MEM-136)

Western Blot: HLA DR DP Antibody (MEM-136) [NB500-447] - Fig. 1. Western Blotting analysis of MHC Class II in whole cell lysate of RAJI human Burkitt lymphoma cell line using anti-human HLA-DR+DP (MEM-136). The antibody MEM-136 recognizes beta-chain of HLA-DR antigen under non-reducing conditions. Lane 1: immunostaining with anti-human HLA-DR+DP (MEM-136) Lane 2: immunostaining with Isotype mouse IgG1 control (PPV-06).

• Reactivity: Hu
• Applications: WB, Flow, IHC, IHC-P, IP, CyTOF-ready
• Clone: MEM-136
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

HLA DR/DP Antibody (MEM-136) Summary

• Immunogen: PHA-activated peripheral blood lymphocytes.
• Specificity: The antibody MEM-136 recognizes common epitope on beta-chain of human HLA-DR and HLA-DP. It reacts with alpha/beta dimer as well as with dissociated beta-subunit. DR and DP are the isotypes of human MHC Class II molecules expressed on antigen-presenting cells (APC; dendritic cells, B lymphocytes, monocytes, macrophages).
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: HLA-DRA
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
  • Flow Cytometry 0.5 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:50
  • CyTOF-ready
• Application Notes: Western Blot - Non-reducing conditions. This antibody is CyTOF ready.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4)
• Preservative: 15mM Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for HLA DR/DP Antibody (MEM-136)

• FLJ51114
• histocompatibility antigen HLA-DR alpha
• HLA class II histocompatibility antigen, DR alpha chain
• HLA-DRA1
• major histocompatibility complex, class II, DR alpha
• MHC cell surface glycoprotein
• MHC class II antigen DRA
• MLRW

Background

Human MHC class II antigens are transmembrane glycoproteins composed of an alpha (36 kDa) and a beta chain (27kDa) and are expressed primarily on antigen presenting cells. Human MHC class II genes are located in the HLA-D region that encodes at least 6 alpha and 10 beta chain genes. Three loci, DR, DQ and DP, encode the major products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides and present them to T-helper cells and therefore have a critical role in the initiation of the immune response. It has been shown that some autoimmune diseases are associated with certain class II alleles.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HLA-DRA
• Entrez:
  • 3122(Human)