HLA DR/DP Antibody (HL-38) - BSA Free

Flow Cytometry: HLA DR/DP Antibody (HL-38) [NB500-406] - Staining of human peripheral blood with anti-HLA-DR+DP (HL-38) FITC.

Flow Cytometry: HLA DR/DP Antibody (HL-38) [NB500-406] - Surface staining of human peripheral blood with anti-HLA-DR+DP (HL-38) purified, GAM-APC.

Flow Cytometry: HLA DR/DP Antibody (HL-38) [NB500-406] - Staining of human peripheral blood with anti-HLA-DR+DP (HL-38) PE.

• Reactivity: Hu, Po
• Applications: WB, Flow, IHC, IP, CyTOF-ready
• Clone: HL-38
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

HLA DR/DP Antibody (HL-38) - BSA Free Summary

• Immunogen: Raji Burkitt's lymphoma cell line
• Epitope: This antibody binds to a common epitope on the b-chain of human HLA-DR and HLA-DP.
• Specificity: The antibody HL-38 recognizes common epitope on beta-chain of human HLA-DR and HLA-DP. DR and DP are the isotypes of human MHC Class II molecules expressed on antigen-presenting cells (APC; dendritic cells, B lymphocytes, monocytes, macrophages).
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: HLA-DRA
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1-4 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:50
  • Western Blot 1:1000
• Application Notes: Western Blot: use non-reducing conditions. This antibody is CyTOF ready.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for HLA DR/DP Antibody (HL-38) - BSA Free

• FLJ51114
• histocompatibility antigen HLA-DR alpha
• HLA class II histocompatibility antigen, DR alpha chain
• HLA-DRA1
• major histocompatibility complex, class II, DR alpha
• MHC cell surface glycoprotein
• MHC class II antigen DRA
• MLRW

Background

Human MHC class II antigens are transmembrane glycoproteins composed of an alpha (36 kDa) and a beta chain (27kDa) and are expressed primarily on antigen presenting cells. Human MHC class II genes are located in the HLA-D region that encodes at least 6 alpha and 10 beta chain genes. Three loci, DR, DQ and DP, encode the major products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides and present them to T-helper cells and therefore have a critical role in the initiation of the immune response. It has been shown that some autoimmune diseases are associated with certain class II alleles.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HLA DR/DP Antibody (NB500-406)(1)

Publication using NB500-406

• Schimunek L, Serve R, Teuben MPJ et al. Early decreased TLR2 expression on monocytes is associated with their reduced phagocytic activity and impaired maturation in a porcine polytrauma model PLoS ONE. 2017-11-10 [PMID: 29125848] (FLOW, Porcine)

Bioinformatics

• Gene Symbol: HLA-DRA
• Entrez:
  • 3122(Human)