HLA DQA2 Antibody

Images

 
Western Blot: HLA DQA2 Antibody [NBP1-52186] - (0.3ug/ml) staining of Human Bone Marrow lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: HLA DQA2 Antibody [NBP1-52186] - 3.75ug/ml staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer Ph 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

HLA DQA2 Antibody Summary

Immunogen
Peptide with sequence C-QSHGPSGQYTH corresponding to internal region according to NP_064440.1.
Epitope
C-QSHGPSGQYTH
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
HLA-DQA2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 0.1-0.3 ug/ml
  • Immunohistochemistry-Paraffin
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.1-0.3ug/ml
Application Notes
Western blot: Approx 26kDa band observed in Human Bone Marrow lysates (calculated MW of 28.0kDa according to NP_064440.1). Primary incubation was 1 hour
IHC: Paraffin embedded Human Spleen.
Publications
Read Publication using NBP1-52186.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for HLA DQA2 Antibody

  • DX alpha chain
  • DX-ALPHA
  • HLA class II histocompatibility antigen, DQ alpha 2 chain
  • HLA class II histocompatibility antigen, DQ(6) alpha chain
  • HLA-DQA1
  • HLA-DXAMHC class II DQA2
  • major histocompatibility complex, class II, DQ alpha 2

Background

The HLA-DQA2 gene encodes a 255 amino acid long, 28 kDA HLA class II histocompatibility antigen, DQ alpha 2 chain that functions in the peptide loading of MHC class II molecules. It does so by releasing the CLIP molecule from the peptide binding site. The HLA-DQA2 gene participates in cell adhesion molecules (CAMs), antigen processing and presentation, transport of MHC II:Ii complex along Golgi to TGN, the CDC42 pathway, and in G-protein signaling in N-RAS regulation pathways. It is known to interact with genes CD4 and HLA-DQB1. HLA-DQA2 is linked to osteoarthritis, celiac disease, diabetes, asthma, thyroiditis, childhood type dermatomyositis, graft vs. host disease, narcolepsy, and lupus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HLA DQA2 Antibody (NBP1-52186)(1)

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol HLA-DQA2
Entrez
Uniprot