HLA DQA2 Antibody

Western Blot: HLA DQA2 Antibody [NBP1-52186] - (0.3ug/ml) staining of Human Bone Marrow lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: HLA DQA2 Antibody [NBP1-52186] - 3.75ug/ml staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer Ph 6, AP-staining.

• Reactivity: Hu
• Applications: WB, IHC, IHC-P, PEP-ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

HLA DQA2 Antibody Summary

• Immunogen: Peptide with sequence C-QSHGPSGQYTH corresponding to internal region according to NP_064440.1.
• Epitope: C-QSHGPSGQYTH
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: HLA-DQA2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 0.1-0.3 ug/ml
  • Immunohistochemistry-Paraffin
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.1-0.3ug/ml
• Application Notes: Western blot: Approx 26kDa band observed in Human Bone Marrow lysates (calculated MW of 28.0kDa according to NP_064440.1). Primary incubation was 1 hour
  IHC: Paraffin embedded Human Spleen.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for HLA DQA2 Antibody

• DX alpha chain
• DX-ALPHA
• HLA class II histocompatibility antigen, DQ alpha 2 chain
• HLA class II histocompatibility antigen, DQ(6) alpha chain
• HLA-DQA1
• HLA-DXAMHC class II DQA2
• major histocompatibility complex, class II, DQ alpha 2

Background

The HLA-DQA2 gene encodes a 255 amino acid long, 28 kDA HLA class II histocompatibility antigen, DQ alpha 2 chain that functions in the peptide loading of MHC class II molecules. It does so by releasing the CLIP molecule from the peptide binding site. The HLA-DQA2 gene participates in cell adhesion molecules (CAMs), antigen processing and presentation, transport of MHC II:Ii complex along Golgi to TGN, the CDC42 pathway, and in G-protein signaling in N-RAS regulation pathways. It is known to interact with genes CD4 and HLA-DQB1. HLA-DQA2 is linked to osteoarthritis, celiac disease, diabetes, asthma, thyroiditis, childhood type dermatomyositis, graft vs. host disease, narcolepsy, and lupus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HLA DQA2 Antibody (NBP1-52186)(1)

Publication using NBP1-52186

• Davila S, Froeling FE, Tan A et al. New genetic associations detected in a host response study to hepatitis B vaccine. Genes Immun 2010-04-01 [PMID: 20237496]

Bioinformatics

• Gene Symbol: HLA-DQA2
• Entrez:
  • 3118(Human)
• Uniprot:
  • NP_064440.1(Human)