HLA DQA2 Antibody

Western Blot: HLA DQA2 Antibody [NBP1-52094] - Analysis of HLA DQA2 in Human Bone Marrow lysate (35ug protein in RIPA buffer) using NBP1-52094 at 1ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

HLA DQA2 Antibody Summary

• Description: Novus Biologicals Goat HLA DQA2 Antibody (NBP1-52094) is a polyclonal antibody validated for use in WB and ELISA. Anti-HLA DQA2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-QGLRSVGASRH corresponding to C-Terminus according to NP_064440.1, NP_002113.2.
• Epitope: C-QGLRSVGASRH
• Specificity: This antibody is expected to recognize: NP_064440.1 (HLA-DQA2; GeneID: 3118) and also the very similar NP_002113.2 (HLA-DQA1; GeneID:3117).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: HLA-DQA2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 28 kDa band observed in human bone marrow lysates (calculated MW of 28.0 kDa band according to NP_064440.1).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for HLA DQA2 Antibody

• DX alpha chain
• DX-ALPHA
• HLA class II histocompatibility antigen, DQ alpha 2 chain
• HLA class II histocompatibility antigen, DQ(6) alpha chain
• HLA-DQA1
• HLA-DXAMHC class II DQA2
• major histocompatibility complex, class II, DQ alpha 2

Background

The HLA-DQA2 gene encodes a 255 amino acid long, 28 kDA HLA class II histocompatibility antigen, DQ alpha 2 chain that functions in the peptide loading of MHC class II molecules. It does so by releasing the CLIP molecule from the peptide binding site. The HLA-DQA2 gene participates in cell adhesion molecules (CAMs), antigen processing and presentation, transport of MHC II:Ii complex along Golgi to TGN, the CDC42 pathway, and in G-protein signaling in N-RAS regulation pathways. It is known to interact with genes CD4 and HLA-DQB1. HLA-DQA2 is linked to osteoarthritis, celiac disease, diabetes, asthma, thyroiditis, childhood type dermatomyositis, graft vs. host disease, narcolepsy, and lupus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HLA DQA2 Antibody (NBP1-52094)(1)

Publication using NBP1-52094

• Davila S, Froeling FE, Tan A et al. New genetic associations detected in a host response study to hepatitis B vaccine. Genes Immun 2010-04-01 [PMID: 20237496]

Bioinformatics

• Gene Symbol: HLA-DQA2
• Uniprot:
  • NP_064440.1(Human)