HLA DMB Antibody (4D12) - Azide and BSA Free

Western Blot: HLA DMB Antibody (4D12) [H00003109-M08] - Analysis of HLA-DMB expression in transfected 293T cell line by HLA-DMB monoclonal antibody (M08), clone 4D12. Lane 1: HLA-DMB transfected lysatE (28.9 KDa). Lane 2: Non-transfected lysate.

Immunoprecipitation: HLA DMB Antibody (4D12) [H00003109-M08] - Analysis of HLA-DMB transfected lysate using anti-HLA-DMB monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with HLA-DMB rabbit polyclonal antibody.

• Reactivity: Hu
• Applications: WB, ELISA, IP
• Clone: 4D12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

HLA DMB Antibody (4D12) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: HLA-DMB (NP_002109, 22 a.a. ~ 129 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. VAHVESTCLLDDAGTPKDFTYCISFNKDLLTCWDPEENKMAPCEFGVLNSLANVLSQHLNQKDTLMQRLRNGLQNCATHTQPFWGSLTNRTRPPSVQVAKTTPFNTRE
• Specificity: HLA-DMB - major histocompatibility complex, class II, DM beta (4D12)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: HLA-DMB
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunoprecipitation
  • Western Blot 1:500
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for HLA DMB Antibody (4D12) - Azide and BSA Free

• class II histocompatibility antigen, M beta chain
• D6S221E
• DMB
• major histocompatibility complex, class II, DM beta
• MHC class II antigen DMB
• MHC class II antigen HLA-DM beta chain
• MHC class II HLA-DMB
• Really interesting new gene 7 protein
• RING7HLA class II histocompatibility antigen, DM beta chain

Background

HLA-DMB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DMA) and a beta (DMB) chain, both anchored in the membrane. It is located in intracellular vesicles. DM plays a central role in the peptide loading of MHC class II molecules by helping to release the CLIP (class II-associated invariant chain peptide) molecule from the peptide binding site. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HLA-DMB
• Uniprot:
  • NP_002109(Human)