HLA B7 Antibody

Images

 
Western Blot: HLA B7 Antibody [NBP2-29931] - Analysis of lysates from HL-60, Jurkat, Ramos cell line (from left to right), using HLA-B Antibody (N-term). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) ...read more
Immunohistochemistry-Paraffin: HLA B7 Antibody [NBP2-29931] - Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with HLA-B Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, ...read more
Flow Cytometry: HLA B7 Antibody [NBP2-29931] - Analysis of human peripheral blood lymphocytes cells using HLA-B Antibody (N-term) (green, compared to an isotype control of rabbit IgG (blue). Diluted at 1:25 dilution. An ...read more
Western Blot: HLA B7 Antibody [NBP2-29931] - Western blot analysis of HLA-B Antibody (N-term) (NBP2-29931) in A2058 cell line lysates (35ug/lane). HLA-B (arrow) was detected using the purified Pab.
Western Blot: HLA B7 Antibody [NBP2-29931] - Western blot analysis of HLA-B (arrow) using rabbit polyclonal HLA-B Antibody (N-term) (NBP2-29931 ). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or ...read more
Western Blot: HLA B7 Antibody [NBP2-29931] - Western blot analysis of HLA-B (arrow) using rabbit polyclonal HLA-B Antibody (N-term) (NBP2-29931 ). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or ...read more
Western Blot: HLA B7 Antibody [NBP2-29931] - Analysis of lysates from CEM, HL-60, Jurkat, Ramos cell line (from left to right), using HLA-B Antibody (N-term. Diluted at 1:1000 at each lane. A goat anti-rabbit IgG ...read more
Flow Cytometry: HLA B7 Antibody [NBP2-29931] - Flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

HLA B7 Antibody Summary

Immunogen
This HLA-B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 62-90 amino acids from the N-terminal region of human HLA-B.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HLA-B
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Flow Cytometry 1:10 - 1:50
  • Immunohistochemistry-Paraffin 1:10 - 1:50
Theoretical MW
40.428 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A purified

Alternate Names for HLA B7 Antibody

  • ankylosing spondylitis
  • AS
  • b'DT
  • Bw-22
  • Bw-4
  • Bw-41
  • Bw-44
  • Bw-45
  • Bw-46
  • Bw-47
  • Bw-48
  • Bw-50
  • Bw-52
  • Bw-53
  • Bw-54
  • Bw-55
  • Bw-56
  • Bw-57
  • Bw-58
  • Bw-60
  • HLA class I histocompatibility antigen, B alpha chain
  • HLA class I histocompatibility antigen, B-12 alpha chain
  • HLA class I histocompatibility antigen, B-21 alpha chain
  • HLA class I histocompatibility antigen, B-5 alpha chain
  • HLA class I histocompatibility antigen, B-7 alpha chain
  • HLAB
  • HLA-B27
  • HLA-B73
  • leukocyte antigen class I-B
  • lymphocyte antigen
  • major histocompatibility complex, class I, B
  • MGC111087
  • MHC class I antigen B*13
  • MHC class I antigen B*14
  • MHC class I antigen B*15
  • MHC class I antigen B*18
  • MHC class I antigen B*27
  • MHC class I antigen B*35
  • MHC class I antigen B*37
  • MHC class I antigen B*38
  • MHC class I antigen B*39
  • MHC class I antigen B*40
  • MHC class I antigen B*41
  • MHC class I antigen B*42
  • MHC class I antigen B*44
  • MHC class I antigen B*45
  • MHC class I antigen B*46
  • MHC class I antigen B*47
  • MHC class I antigen B*48
  • MHC class I antigen B*49
  • MHC class I antigen B*50
  • MHC class I antigen B*51
  • MHC class I antigen B*52
  • MHC class I antigen B*53
  • MHC class I antigen B*54
  • MHC class I antigen B*55
  • MHC class I antigen B*56
  • MHC class I antigen B*57
  • MHC class I antigen B*58
  • MHC class I antigen B*59
  • MHC class I antigen B*67
  • MHC class I antigen B*7
  • MHC class I antigen B*73
  • MHC class I antigen B*78
  • MHC class I antigen B*8
  • MHC class I antigen B*81
  • MHC class I antigen B*82
  • MHC class I antigen GN00104
  • MHC class I antigen HLA-B heavy chain
  • MHC class I antigen SHCHA
  • MHC Class I HLA heavy chain
  • MHC HLA-B cell surface glycoprotein
  • SPDA1

Background

HLA-B is involved in the presentation of foreign antigens to the immune system.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional Array Products

Array NBP2-29931

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Bioinformatics

Gene Symbol HLA-B
Uniprot