HLA B Overexpression Lysate (Native)

Images

 
Western Blot: HLA B Overexpression Lysate (Adult Normal) [NBL1-11585] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HLA B.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

HLA B Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC210631 and based on accession number NM_005514. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens major histocompatibility complex, class I, B (HLA-B), mRNA.
Gene
HLA-B

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
37.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for HLA B

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for HLA B Overexpression Lysate (Native)

  • ankylosing spondylitis
  • AS
  • b'DT
  • Bw-22
  • Bw-4
  • Bw-41
  • Bw-44
  • Bw-45
  • Bw-46
  • Bw-47
  • Bw-48
  • Bw-50
  • Bw-52
  • Bw-53
  • Bw-54
  • Bw-55
  • Bw-56
  • Bw-57
  • Bw-58
  • Bw-60
  • HLA class I histocompatibility antigen, B alpha chain
  • HLA class I histocompatibility antigen, B-12 alpha chain
  • HLA class I histocompatibility antigen, B-21 alpha chain
  • HLA class I histocompatibility antigen, B-5 alpha chain
  • HLA class I histocompatibility antigen, B-7 alpha chain
  • HLAB
  • HLA-B27
  • HLA-B73
  • leukocyte antigen class I-B
  • lymphocyte antigen
  • major histocompatibility complex, class I, B
  • MGC111087
  • MHC class I antigen B*13
  • MHC class I antigen B*14
  • MHC class I antigen B*15
  • MHC class I antigen B*18
  • MHC class I antigen B*27
  • MHC class I antigen B*35
  • MHC class I antigen B*37
  • MHC class I antigen B*38
  • MHC class I antigen B*39
  • MHC class I antigen B*40
  • MHC class I antigen B*41
  • MHC class I antigen B*42
  • MHC class I antigen B*44
  • MHC class I antigen B*45
  • MHC class I antigen B*46
  • MHC class I antigen B*47
  • MHC class I antigen B*48
  • MHC class I antigen B*49
  • MHC class I antigen B*50
  • MHC class I antigen B*51
  • MHC class I antigen B*52
  • MHC class I antigen B*53
  • MHC class I antigen B*54
  • MHC class I antigen B*55
  • MHC class I antigen B*56
  • MHC class I antigen B*57
  • MHC class I antigen B*58
  • MHC class I antigen B*59
  • MHC class I antigen B*67
  • MHC class I antigen B*7
  • MHC class I antigen B*73
  • MHC class I antigen B*78
  • MHC class I antigen B*8
  • MHC class I antigen B*81
  • MHC class I antigen B*82
  • MHC class I antigen GN00104
  • MHC class I antigen HLA-B heavy chain
  • MHC class I antigen SHCHA
  • MHC Class I HLA heavy chain
  • MHC HLA-B cell surface glycoprotein
  • SPDA1

Background

HLA-B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exon 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-B alleles have been described. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for HLA B Lysate (NBL1-11585) (0)

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Bioinformatics

Gene Symbol HLA-B