HLA B Antibody (11C2)

Images

 
Western Blot: HLA B Antibody (11C2) [NBP2-76879] - Western blot analysis of HLA Class 1 ABC on Hela cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room ...read more
Immunocytochemistry: HLA B Antibody (11C2) [NBP2-76879] - ICC staining HLA Class 1 ABC in 293T cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and ...read more
Immunohistochemistry: HLA B Antibody (11C2) [NBP2-76879] - Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-HLA Class 1 ABC antibody. The section was pre-treated using heat mediated ...read more
Immunohistochemistry: HLA B Antibody (11C2) [NBP2-76879] - Immunohistochemical analysis of paraffin-embedded rat uterus tissue using anti-HLA Class 1 ABC antibody. The section was pre-treated using heat mediated antigen ...read more
Immunohistochemistry: HLA B Antibody (11C2) [NBP2-76879] - Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-HLA Class 1 ABC antibody. The section was pre-treated using heat mediated ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clone
11C2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
2 mg/ml

Order Details

HLA B Antibody (11C2) Summary

Immunogen
Recombinant protein within Human HLA B aa 1-362 / 362. (SwissProt: P01889 Human; SwissProt: P30443 Human; SwissProt: P30499 Human)
Localization
Cell membrane.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
HLA-B
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:50
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2000
Theoretical MW
40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-questions.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH7.4), 0.2% BSA, 50% Glycerol
Preservative
0.05% Sodium Azide
Concentration
2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for HLA B Antibody (11C2)

  • ankylosing spondylitis
  • AS
  • B-4901
  • b'DT
  • Bw-22
  • Bw-4
  • Bw-41
  • Bw-44
  • Bw-45
  • Bw-46
  • Bw-47
  • Bw-48
  • Bw-50
  • Bw-52
  • Bw-53
  • Bw-54
  • Bw-55
  • Bw-56
  • Bw-57
  • Bw-58
  • Bw-60
  • HLA class I histocompatibility antigen, B alpha chain
  • HLA class I histocompatibility antigen, B-12 alpha chain
  • HLA class I histocompatibility antigen, B-21 alpha chain
  • HLA class I histocompatibility antigen, B-5 alpha chain
  • HLA class I histocompatibility antigen, B-7 alpha chain
  • HLAB
  • HLA-B
  • HLA-B27
  • HLA-B73
  • leukocyte antigen class I-B
  • lymphocyte antigen
  • major histocompatibility complex, class I, B
  • MGC111087
  • MHC class I antigen B*13
  • MHC class I antigen B*14
  • MHC class I antigen B*15
  • MHC class I antigen B*18
  • MHC class I antigen B*27
  • MHC class I antigen B*35
  • MHC class I antigen B*37
  • MHC class I antigen B*38
  • MHC class I antigen B*39
  • MHC class I antigen B*40
  • MHC class I antigen B*41
  • MHC class I antigen B*42
  • MHC class I antigen B*44
  • MHC class I antigen B*45
  • MHC class I antigen B*46
  • MHC class I antigen B*47
  • MHC class I antigen B*48
  • MHC class I antigen B*49
  • MHC class I antigen B*50
  • MHC class I antigen B*51
  • MHC class I antigen B*52
  • MHC class I antigen B*53
  • MHC class I antigen B*54
  • MHC class I antigen B*55
  • MHC class I antigen B*56
  • MHC class I antigen B*57
  • MHC class I antigen B*58
  • MHC class I antigen B*59
  • MHC class I antigen B*67
  • MHC class I antigen B*7
  • MHC class I antigen B*73
  • MHC class I antigen B*78
  • MHC class I antigen B*8
  • MHC class I antigen B*81
  • MHC class I antigen B*82
  • MHC class I antigen GN00104
  • MHC class I antigen HLA-B heavy chain
  • MHC class I antigen SHCHA
  • MHC Class I HLA heavy chain
  • MHC HLA-B cell surface glycoprotein
  • SPDA1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol HLA-B