HIV-1 Rev binding protein (HRB) Overexpression Lysate (Native)

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Western Blot: HIV-1 Rev binding protein (HRB) Overexpression Lysate (Adult Normal) [NBL1-11705] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HIV-1 Rev binding ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

HIV-1 Rev binding protein (HRB) Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC205375 and based on accession number NM_004504. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens ArfGAP with FG repeats 1 (AGFG1), transcript variant 2, mRNA.
Gene
AGFG1

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
58.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for HIV-1 Rev binding protein (HRB)

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for HIV-1 Rev binding protein (HRB) Overexpression Lysate (Native)

  • ArfGAP with FG repeats 1
  • DKFZp686I15205
  • HIV-1 Rev-binding protein
  • HRBRev interacting protein
  • Rab, Rev/Rex activation domain-binding protein
  • RABMGC116938
  • RIPMGC116940

Background

The protein encoded by this gene is related to nucleoporins, a class of proteins that mediate nucleocytoplasmic transport. The encoded protein binds the activation domain of the human immunodeficiency virus Rev protein when Rev is assembled onto its RNA target, and is required for the nuclear export of Rev-directed RNAs. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]. Transcript Variant: This variant (2) lacks an alternate in-frame exon and uses an alternate in-frame splice site in the 3' coding region, compared to variant 1, resulting in a shorter protein (isoform 2), compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for HIV-1 Rev binding protein (HRB) Lysate (NBL1-11705) (0)

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Bioinformatics

Gene Symbol AGFG1