Histone H4 [Monomethyl Lys20] Antibody [DyLight 550]

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Product Details

Summary
Reactivity Hu, Mu, Ce, Chicken, Drosophila, Plant, Rat, XenopusSpecies Glossary
Applications WB, ChIP, DB, ICC/IF
Clonality
Polyclonal
Host
Rabbit

Histone H4 [Monomethyl Lys20] Antibody [DyLight 550] Summary

Immunogen
Synthetic monomethylated peptide surrounding Lysine 20 of human Histone H4 [Swiss Prot P62805].
Modification
Monomethyl Lys20
Localization
Nucleus. Chromosome.
Marker
H4K20me1
Predicted Species
Rat (100%), Plant (100%), Xenopus (100%), Chicken (100%), Drosophila (100%). Backed by our 100% Guarantee.
Clonality
Polyclonal
Host
Rabbit
Gene
HIST4H4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Conjugate
DyLight 550
Format
DyLight 550 (A=562, E=576)
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:1:500
  • Chromatin Immunoprecipitation 2-5 ug per million cells
  • Dot Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:50
Application Notes
This Histone H4 K20me1 antibody is useful for Immunocytochemistry/Immunofluorescence,ChIP, Dot blot, and Western blot where a band is seen ~13kDa.

Reactivity Notes

Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology.

Alternate Names for Histone H4 [Monomethyl Lys20] Antibody [DyLight 550]

  • H4/A
  • H4/B
  • H4/C
  • H4/D
  • H4/E
  • H4/G
  • H4/H
  • H4/I
  • H4/J
  • H4/K
  • H4/M
  • H4/N
  • H4/O
  • H4/p
  • H4F2
  • H4FA
  • H4FB
  • H4FC
  • H4FD
  • H4FE
  • H4FG
  • H4FH
  • H4FI
  • H4FJ
  • H4FK
  • H4FM
  • H4FN
  • H4FO
  • HIST1H4A
  • HIST1H4B
  • HIST1H4C
  • HIST1H4D
  • HIST1H4E
  • HIST1H4F
  • HIST1H4H
  • HIST1H4I
  • HIST1H4J
  • HIST1H4K
  • HIST1H4L
  • HIST2H4
  • HIST2H4A
  • HIST2H4B
  • HIST4H4
  • histone 4, H4
  • histone cluster 4, H4
  • Histone H4
  • MGC24116

Background

SET8 specifically catalyzes the mono-methylation of H4 at K20. Loss of this crucial methylation causes multiple DNA breaks, which instigates a p53-dependent DNA damage response to avoid mitosis and aberrant chromosomal activity. Therefore, this PTM is essential to genome replication and stability through S-phase. In mammalian stem cells, Xist expression blocks the formation of H4K20me1, which is one of the first examples of a direct connection between chromatin and stem cell differentiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for Histone H4 Antibody (NB21-2088R) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Histone H4 Antibody (NB21-2088R)

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Blogs on Histone H4.

H4 - Monitoring global chromatin structure through histone modifications
Histones make up the main protein component of chromatin and are responsible for storing and organizing the genome in a compact yet accessible manner. In addition to storage, histones play an important role in the regulation of various cellular pro...  Read full blog post.

Histone H4 Phosphorylation: Affecting Liver Regeneration and Cancer
Histones are highly conserved proteins that function in the organization of nuclear DNA to create chromatin in eukaryotic cells. Post-translational alterations of histones are critical to monitoring and regulating DNA structure, expression, and gene t...  Read full blog post.

Histone H4: Implications in Liver Cancer
Histones are highly conserved proteins that function in the organization of nuclear DNA to create chromatin in eukaryotic cells. Post-translational alterations of histones are critical to monitoring and regulating DNA structure, expression, and gene t...  Read full blog post.

"Come Fly with Me" - New Drosophila Model Developed for Direct in Vivo Study of Histones
Forming the major protein component of chromatin, histones are essential to the structure and organization of chromosomes, forming the nucleosome around which DNA is packaged and wrapped.Antibody studies have revealed histones undergo various posttr...  Read full blog post.

Contact Information

Product PDFs

Bioinformatics

Gene Symbol HIST4H4
Entrez

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