![Western Blot: Histone H4 [ac Lys12] Antibody [NB21-2064] - Analysis of NIH 3T3 histone prep lysate (A), and HeLa histone prep lysate (B) using Histone H4 [ac Lys12] antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys12]-Antibody-Western-Blot-NB21-2064-img0003.jpg "Western Blot: Histone H4 [ac Lys12] Antibody [NB21-2064] - Analysis of NIH 3T3 histone prep lysate (A), and HeLa histone prep lysate (B) using Histone H4 [ac Lys12] antibody at 1 ug/ml.")
| Immunogen | Histone H4 |
| Modification | ac Lys12 |
| Localization | Nucleus. Chromosome |
| Marker | H4R3me1 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | H4C16 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 20mM Potassium Phosphate (pH 7.2) and 0.15M NaCl |
| Preservative | 0.01% Sodium Azide |
| Concentration | 0.69 mg/ml |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Histone H4 Antibody (NB21-2064)Find related products by research area.
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H4 - Monitoring global chromatin structure through histone modifications Histones make up the main protein component of chromatin and are responsible for storing and organizing the genome in a compact yet accessible manner. In addition to storage, histones play an important role in the regulation of various cellular pro... Read full blog post. |
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Histone H4 Phosphorylation: Affecting Liver Regeneration and Cancer Histones are highly conserved proteins that function in the organization of nuclear DNA to create chromatin in eukaryotic cells. Post-translational alterations of histones are critical to monitoring and regulating DNA structure, expression, and gene t... Read full blog post. |
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Histone H4: Implications in Liver Cancer Histones are highly conserved proteins that function in the organization of nuclear DNA to create chromatin in eukaryotic cells. Post-translational alterations of histones are critical to monitoring and regulating DNA structure, expression, and gene t... Read full blog post. |
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![Immunocytochemistry/Immunofluorescence: Histone H4 [ac Lys12] Antibody [NB21-2064] - Tested at 1:10 in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). Image objective 40x.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys12]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-2064-img0002.jpg "Immunocytochemistry/Immunofluorescence: Histone H4 [ac Lys12] Antibody [NB21-2064] - Tested at 1:10 in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). Image objective 40x.")
![Immunohistochemistry-Paraffin: Histone H4 [ac Lys12] Antibody [NB21-2064] - Analysis of FFPE human tonsil using Histone H4 [ac Lys12] antibody at 1:500 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Nuclear staining was observed. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys12]-Antibody-Immunohistochemistry-Paraffin-NB21-2064-img0004.jpg "Immunohistochemistry-Paraffin: Histone H4 [ac Lys12] Antibody [NB21-2064] - Analysis of FFPE human tonsil using Histone H4 [ac Lys12] antibody at 1:500 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Nuclear staining was observed. Staining was performed by Histowiz.")
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