Histone H4 [Methyl Lys20] Antibody (C.15100147)

Western Blot: Histone H4 [Methyl Lys20] Antibody (C.15100147) [NBP2-59234] - Histone extracts of HeLa cells (15 ug) were analyzed by Western blot using the antibody against H4K20me1 at 1:1000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.

Chromatin Immunoprecipitation: Histone H4 [Methyl Lys20] Antibody (C.15100147) [NBP2-59234] - ChIP assays were performed using human HeLa cells, the antibody against H4K20me1 and optimized PCR primer sets for qPCR. ChIP was performed with a LowCell number ChIP kit, using sheared chromatin from 10,000 cells. Two different quantities of antibody (3 and 12 uL per ChIP experiment) were analyzed. IgG (1 ug) was used as negative IP control. QPCR was performed with primers for the GAPDH promoter and for the inactive gene TSH2B. Figure shows the recovery, expressed as % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

Dot Blot: Histone H4 [Methyl Lys20] Antibody (C.15100147) [NBP2-59234] - To check the specificity of the antibody against H4K20me1 a Dot Blot was performed with peptides containing different modifications or unmodified sequences of histone H4. 100 to 0.2 pmol of peptide containing the respective histone modification were spotted on a membrane. The antibody was used at a dilution of 1:10,000. Figure shows a high specificity of the antibody for the modification of interest.

Product Discontinued

Histone H4 [Methyl Lys20] Antibody (C.15100147) Summary

• Description: Monoclonal antibody raised in mouse against histone H4 containing the monomethylated lysine 20 (H4K20me1), using a KLH-conjugated synthetic peptide.
• Immunogen: The exact immunogen is propietary information.
• Modification: Methyl Lys20
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: HIST4H4
• Purity: Concentrated Supernatant

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation 3 uL
  • Dot Blot 1:10000
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Culture Medium
• Preservative: 0.05% Sodium Azide
• Purity: Concentrated Supernatant

Alternate Names for Histone H4 [Methyl Lys20] Antibody (C.15100147)

• H4
• H4/A
• H4/B
• H4/C
• H4/D
• H4/E
• H4/G
• H4/H
• H4/I
• H4/J
• H4/K
• H4/M
• H4/N
• H4/O
• H4/p
• H4F2
• H4FA
• H4FB
• H4FC
• H4FD
• H4FE
• H4FG
• H4FH
• H4FI
• H4FJ
• H4FK
• H4FM
• H4FN
• H4FO
• H4K20Me
• H4M
• HIST1H4A
• HIST1H4B
• HIST1H4C
• HIST1H4D
• HIST1H4E
• HIST1H4F
• HIST1H4H
• HIST1H4I
• HIST1H4J
• HIST1H4K
• HIST1H4L
• HIST2H4
• HIST2H4A
• HIST2H4B
• HIST4H4
• histone 4, H4
• Histone Cluster 1 H4
• Histone Cluster 1 H4i
• Histone Cluster 4 H4
• histone cluster 4, H4
• Histone H4
• MGC24116

Background

Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called histone code. Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HIST4H4