Histone H3 [Trimethyl Lys27] Antibody

Images

 
Western Blot: Histone H3 [Trimethyl Lys27] Antibody [NBP2-16840] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3K27me3 (trimethyl Lys27) antibody ...read more
Immunocytochemistry/ Immunofluorescence: Histone H3 [Trimethyl Lys27] Antibody [NBP2-16840] - Immunofluorescence photomicrographs of frozen sections of mouse liver. Red: Histone H3K27me3 (trimethyl Lys27) antibody ...read more
Immunohistochemistry Whole-Mount: Histone H3 [Trimethyl Lys27] Antibody [NBP2-16840] - Paraformaldehyde-fixed 2 day-post-fertilization zebrafish embryo. Histone H3K27me3 (trimethyl Lys27) antibody dilution: 1:100.
Western Blot: Histone H3 [Trimethyl Lys27] Antibody [NBP2-16840] - Histone H3 [Trimethyl Lys27] Antibody U87 cells were grown under normoxic (DMEM 10% FBS, 16% O2), hypoxic (DMEM 10% FBS, 1% O2), and neurosphere ...read more
Western Blot: Histone H3 [Trimethyl Lys27] Antibody [NBP2-16840] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3 (tri-Methyl Lys27) antibody diluted at ...read more
Western Blot: Histone H3 [Trimethyl Lys27] Antibody [NBP2-16840] - Sample (30 ug of whole cell lysate) A: Jurkat, B: Raji, C: K562, D: THP-1, 7.5% SDS PAGE diluted at 1:500
Immunocytochemistry/ Immunofluorescence: Histone H3 [Trimethyl Lys27] Antibody [NBP2-16840] - Histone H3 [Trimethyl Lys27] Antibody Confocal immunofluorescence analysis of paraformaldehyde-fixed A431, using HIST1H3A ...read more
Immunohistochemistry-Paraffin: Histone H3 [Trimethyl Lys27] Antibody [NBP2-16840] - Histone H3 [Trimethyl Lys27] Antibody Immunohistochemical analysis of paraffin-embedded Hela xenograft, using antibody at 1:500 ...read more
Immunohistochemistry-Paraffin: Histone H3 [Trimethyl Lys27] Antibody [NBP2-16840] - Paraffin-embedded mouse brain Histone H3K27me3 (trimethyl Lys27) antibody diluted at 1:500.
Immunohistochemistry-Paraffin: Histone H3 [Trimethyl Lys27] Antibody [NBP2-16840] - Paraffin-embedded mouse duodenum Histone H3K27me3 (trimethyl Lys27) antibody diluted at 1:500
Dot Blot: Histone H3 [Trimethyl Lys27] Antibody [NBP2-16840] - Various peptide samples were spotted onto positively charged nylon membrane, and blotted with Histone H3 (tri-Methyl Lys27) antibody diluted at 1:2500.

Product Details

Summary
Reactivity Hu, Mu, Rt, Ze, PmSpecies Glossary
Applications WB, DB, EM, ICC/IF, IHC, IHC-Fr, IHC-P, IHC-WhMt
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Histone H3 [Trimethyl Lys27] Antibody Summary

Immunogen
The exact sequence is proprietary.
Modification
Trimethyl Lys27
Localization
Nucleus
Predicted Species
Primate (93%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HIST1H3A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Dot Blot 1:100 - 1:2000
  • Electron Microscopy 1:10 - 1:500
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Frozen 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunohistochemistry Whole-Mount
Theoretical MW
15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP2-16840 in the following applications:

Reactivity Notes

Chimpanzee (93%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0), 1.0% BSA and 20% Glycerol
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for Histone H3 [Trimethyl Lys27] Antibody

  • H3 histone, family 3A
  • H3.3AH3F3H3F3B
  • H3.3B
  • H3F3A
  • H3K27Me3
  • H3pS28K27Me3
  • Histone H3
  • histone H3.3
  • MGC87782
  • MGC87783

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-81964
Species: Hu, Mu, Rt
Applications: WB, ICC/IF
NBP2-07993
Species: Hu
NBP2-52487
Species: Hu
Applications: WB, ELISA, Flow, IHC, CyTOF-ready, ICC, IF
NBP2-07996
Species: Hu
NBP1-30141
Species: Hu, Mu, Rt, Po, Ce, Dr, In, Ma, Ye
Applications: WB, ChIP, ELISA, ICC/IF, IHC, IHC-P, IP
H00008349-M06
Species: Hu
Applications: ELISA, IHC-P
H00008337-P01
Species: Hu
NBP2-67336
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-85351
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NB100-56346
Species: Hu, Mu
Applications: WB, ELISA, IHC-P
H00008294-P01
Species: Hu
NB21-2024
Species: Hu, Mu, Rt, Ma
Applications: WB, ChIP, DB, ICC/IF
H00008330-Q01
Species: Hu
NBP2-16840
Species: Hu, Mu, Rt, Ze
Applications: WB, DB, EM, ICC/IF, IHC, IHC-Fr, IHC-P, IHC-WhMt
H00008351-M01
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF, IHC-P
H00008336-P01
Species: Hu
H00008360-R01
Species: Hu
H00008363-P01
Species: Hu
H00008362-P01
Species: Hu
NBP2-16840
Species: Hu, Mu, Rt, Ze, Pm
Applications: WB, DB, EM, ICC/IF, IHC, IHC-Fr, IHC-P, IHC-WhMt

Publications for Histone H3 Antibody (NBP2-16840) (0)

There are no publications for Histone H3 Antibody (NBP2-16840).
By submitting your publication information earn gift cards and discounts for future purchases.

Review for Histone H3 Antibody (NBP2-16840) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-16840:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot Histone H3 NBP2-16840
Enlarge
reviewed by:
Anonymous
WB Human 06/13/2014
View

Summary

ApplicationWestern Blot
Sample TestedMDA-MB-435 cell lysates
SpeciesHuman

Blocking

Blocking Details5% milk

Primary Anitbody

Dilution Ratio1:1000

Secondary Antibody

Secondary Descriptiongoat anti-rabbit IgG-HRP
Secondary Concentration1:5000

Details

Detection NotesECL prime

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Histone H3 Antibody (NBP2-16840) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Array Products

Bioinformatics Tool for Histone H3 Antibody (NBP2-16840)

Discover related pathways, diseases and genes to Histone H3 Antibody (NBP2-16840). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Research Areas for Histone H3 Antibody (NBP2-16840)

Find related products by research area.

Blogs on Histone H3. Showing 1-10 of 490 blog posts - Show all blog posts.

Article Review: Glucose-induced transcriptional regulation in cancer
Epigenetic mechanisms have been implicated in many physiological and pathophysiological processes. Among these, histone modifications including methylation, phosphorylation, acetylation and ubiquitination, significantly modify gene expression. In c...  Read full blog post.

Article Review: Glucose-induced transcriptional regulation in cancer
Epigenetic mechanisms have been implicated in many physiological and pathophysiological processes. Among these, histone modifications including methylation, phosphorylation, acetylation and ubiquitination, significantly modify gene expression. In c...  Read full blog post.

Article Review: Glucose-induced transcriptional regulation in cancer
Epigenetic mechanisms have been implicated in many physiological and pathophysiological processes. Among these, histone modifications including methylation, phosphorylation, acetylation and ubiquitination, significantly modify gene expression. In c...  Read full blog post.

Article Review: Glucose-induced transcriptional regulation in cancer
Epigenetic mechanisms have been implicated in many physiological and pathophysiological processes. Among these, histone modifications including methylation, phosphorylation, acetylation and ubiquitination, significantly modify gene expression. In c...  Read full blog post.

Article Review: Glucose-induced transcriptional regulation in cancer
Epigenetic mechanisms have been implicated in many physiological and pathophysiological processes. Among these, histone modifications including methylation, phosphorylation, acetylation and ubiquitination, significantly modify gene expression. In c...  Read full blog post.

Article Review: Glucose-induced transcriptional regulation in cancer
Epigenetic mechanisms have been implicated in many physiological and pathophysiological processes. Among these, histone modifications including methylation, phosphorylation, acetylation and ubiquitination, significantly modify gene expression. In c...  Read full blog post.

Article Review: Glucose-induced transcriptional regulation in cancer
Epigenetic mechanisms have been implicated in many physiological and pathophysiological processes. Among these, histone modifications including methylation, phosphorylation, acetylation and ubiquitination, significantly modify gene expression. In c...  Read full blog post.

Article Review: Glucose-induced transcriptional regulation in cancer
Epigenetic mechanisms have been implicated in many physiological and pathophysiological processes. Among these, histone modifications including methylation, phosphorylation, acetylation and ubiquitination, significantly modify gene expression. In c...  Read full blog post.

Article Review: Glucose-induced transcriptional regulation in cancer
Epigenetic mechanisms have been implicated in many physiological and pathophysiological processes. Among these, histone modifications including methylation, phosphorylation, acetylation and ubiquitination, significantly modify gene expression. In c...  Read full blog post.

Article Review: Glucose-induced transcriptional regulation in cancer
Epigenetic mechanisms have been implicated in many physiological and pathophysiological processes. Among these, histone modifications including methylation, phosphorylation, acetylation and ubiquitination, significantly modify gene expression. In c...  Read full blog post.

Showing 1-10 of 490 blog posts - Show all blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Anonymous
06/13/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol HIST1H3A
Entrez