Western Blot: Histone H3 [Trimethyl Lys27] Antibody [NBP2-16840] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3K27me3 (trimethyl Lys27) antibody ...read more
Immunocytochemistry/ Immunofluorescence: Histone H3 [Trimethyl Lys27] Antibody [NBP2-16840] - Immunofluorescence photomicrographs of frozen sections of mouse liver. Red: Histone H3K27me3 (trimethyl Lys27) antibody ...read more
Western Blot: Histone H3 [Trimethyl Lys27] Antibody [NBP2-16840] - Histone H3 [Trimethyl Lys27] Antibody U87 cells were grown under normoxic (DMEM 10% FBS, 16% O2), hypoxic (DMEM 10% FBS, 1% O2), and neurosphere ...read more
Western Blot: Histone H3 [Trimethyl Lys27] Antibody [NBP2-16840] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3 (tri-Methyl Lys27) antibody diluted at ...read more
Western Blot: Histone H3 [Trimethyl Lys27] Antibody [NBP2-16840] - Sample (30 ug of whole cell lysate) A: Jurkat, B: Raji, C: K562, D: THP-1, 7.5% SDS PAGE diluted at 1:500. Observed molecular weight is ~19 kDa.
Immunocytochemistry/ Immunofluorescence: Histone H3 [Trimethyl Lys27] Antibody [NBP2-16840] - Histone H3 [Trimethyl Lys27] Antibody Confocal immunofluorescence analysis of paraformaldehyde-fixed A431, using HIST1H3A ...read more
Immunohistochemistry-Paraffin: Histone H3 [Trimethyl Lys27] Antibody [NBP2-16840] - Histone H3 [Trimethyl Lys27] Antibody Immunohistochemical analysis of paraffin-embedded Hela xenograft, using antibody at 1:500 ...read more
Use in chromatin immunoprecipitation reported in scientific literature (PMID: 30143902).
15 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read 1 Review rated 5 using NBP2-16840 in the following applications:
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
PBS (pH 7), 1% BSA, 20% Glycerol
0.025% Proclin 300
Immunogen affinity purified
Alternate Names for Histone H3 [Trimethyl Lys27] Antibody
H3 histone, family 3A
Chromatin is an arrangement of DNA and proteins which form chromosomes. Chromatin is formed from nucleosomes, which are comprised of a set of four histone proteins (H2A, H2B, H3, H4) wrapped with DNA. Chromatin is very dynamic, where post-translational modifications can regulate DNA to be copied, transcribed, or repaired.
Histones are nuclear proteins responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Changes in chromatin structure play a large role in the regulation of transcription. The chromatin fibers are compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.
Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. Posttranslational modifications such as acetylation of core histones regulates gene expression, thus altering protein function and regulation (1). Histone H3 is primarily acetylated at lysines 9, 14, 18, and 23 and have a theoretical molecular weight of 15 kDa. Acetylation at lysine 9 and 14 appears to control histone deposition, chromatin assembly and active transcription. Methylation of arginine residues within histone H3 has also been linked to transcription regulation. Histone H3 has been linked to various types of cancer as a biomarker through the aberrant expression of histone deacetylase (HDAC) enzymes and changes to chromatins (2-4).
1. Zhang, Y. X., Akumuo, R. C., Espana, R. A., Yan, C. X., Gao, W. J., & Li, Y. C. (2018). The histone demethylase KDM6B in the medial prefrontal cortex epigenetically regulates cocaine reward memory. Neuropharmacology, 141, 113-125. doi:10.1016/j.neuropharm.2018.08.030
2. Nandakumar, V., Hansen, N., Glenn, H. L., Han, J. H., Helland, S., Hernandez, K,...Meldrum, D. R. (2016). Vorinostat differentially alters 3D nuclear structure of cancer and non-cancerous esophageal cells. Sci Rep, 6, 30593. doi:10.1038/srep30593
3. Zhou, M., Li, Y., Lin, S., Chen, Y., Qian, Y., Zhao, Z., & Fan, H. (2019). H3K9me3, H3K36me3, and H4K20me3 Expression Correlates with Patient Outcome in Esophageal Squamous Cell Carcinoma as Epigenetic Markers. Dig Dis Sci, 64(8), 2147-2157. doi:10.1007/s10620-019-05529-2
4. Li, Y., Guo, D., Sun, R., Chen, P., Qian, Q., & Fan, H. (2019). Methylation Patterns of Lys9 and Lys27 on Histone H3 Correlate with Patient Outcome in Gastric Cancer. Dig Dis Sci, 64(2), 439-446. doi:10.1007/s10620-018-5341-8
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.
Publications for Histone H3 Antibody (NBP2-16840)(1)
We have publications tested in 1 confirmed species: Mouse.
We have publications tested in 1 application: ChIP.
The role of DNMT3A in development Epigenetics is the study of heritable change in gene activity despite alteration of the hosts DNA sequence. Change in gene activity done independently of the DNA sequence is achieved by way of histone and DNA methylation. Gene silencing in DNA ... Read full blog post.
Histone H3 Eukaryotic chromosomes are formed through the highly organized and structural wrapping of DNA genetic material around histone proteins into the classic "bead on a string" globular structure of nucleosomes. The histone family consists of five family me... Read full blog post.
EZH2: Epigenetic Regulation Made Easy! Enhancer of Zeste homolog 2 (EZH2) is the methyltransferase enzyme responsible for trimethylating lysine 27 on histone H3 to produce H3K27Me3. EZH2 is a polycomb group protein that is an essential epigenetic regulator that is often found deregulated i... Read full blog post.
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