Histone H3 [p Ser10] Antibody

Images

 
Western Blot: Histone H3 [p Ser10] Antibody [NB21-1091] - WB analysis of H3pS10 in C. elegans embryo lysate.
Immunocytochemistry: Histone H3 [p Ser10] Antibody [NB21-1091] - Immunofluorescence: [NB21-1091] - IF Confocal analysis of HeLa cells using Histone H3 [p Ser10] antibody (NB21-1091, 1:5). An Alexa Fluor 488-conjugated ...read more
Immunohistochemistry-Paraffin: Histone H3 [p Ser10] Antibody [NB21-1091] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human testis using Histone H3 [pS10] antibody at 1:500 on a Bond Rx autostainer (Leica ...read more
Western Blot: Histone H3 [p Ser10] Antibody [NB21-1091] - WB analysis of Histone H3pS10 in HeLa histone prep lysates.
Immunocytochemistry: Histone H3 [p Ser10] Antibody [NB21-1091] - Immunofluorescence: [NB21-1091] - Histone H3pS10 antibody was tested in HeLa cells with FITC (green). Nuclei and actin were counterstained with Dapi ...read more
Dot Blot: Histone H3 [p Ser10] Antibody [NB21-1091] - Dot blot analysis of HIstone H3pS10 with picomoles of peptide.
Histone H3 [p Ser10] Antibody [NB21-1091]

Product Details

Summary
Reactivity Hu, Mu, Rt, Ce, Ch, Dr, Pl, XpSpecies Glossary
Applications WB, DB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.44 mg/ml

Histone H3 [p Ser10] Antibody Summary

Immunogen
Synthetic phosphorylated peptide surrounding Serine 10 of human Histone H3.2 [Swiss Prot Q71DI3].
Modification
p Ser10
Localization
Nucleus. Chromosome.
Marker
Mitosis Marker
Predicted Species
Chicken (100%), Plant (100%), Xenopus (100%), Drosophila (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HIST2H3C
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • Dot Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:2000
  • Immunohistochemistry 1:500-1:1000
  • Immunohistochemistry-Paraffin 1:500-1:1000
Application Notes
This H3 pS10 antibody is useful for ICC/IF, Dot Blot, IHC-P and Western Blot where a band is observed ~15 kDa in HeLa histone prep and C. elegans embryo lysate.
Reviewed Applications
Read 1 Review rated 5
using
NB21-1091 in the following applications:

Reactivity Notes

Human, mouse, and C. elegans. Predicted to react with many species including chicken, Xenopus, Drosophila, and plant based on 100% sequence homology.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
0.44 mg/ml
Purity
Immunogen affinity purified

Notes

Epi-Plus antibody production in collaboration with Rockland Immunochemicals Inc.

Alternate Names for Histone H3 [p Ser10] Antibody

  • H3 histone, family 3A
  • H3.3AH3F3H3F3B
  • H3.3B
  • H3F3A
  • H3S10p
  • Histone H3
  • histone H3.3
  • MGC87782
  • MGC87783

Background

H3 pS10 is typically associated with activation of transcription of genes associated with histone H3. This modification is linked to the initiation of chromatin condensation in G(2). During mitosis, H3pS10 is required for proper chromosome segregation. Formation of the H3pS10 modification seems to be regulated in part by p53, which interacts with histone modifying complexes. In development of the retina, the presence of H3pS10 is linked to the abundance of eye defects. This developmental effect of H3pS10 seems to be related to Psf2 and GPR84. The human Timeless protein (Tim) regulates the global H3pS10 phosphorylation in G2/M phase. VASP concentrations peak during mitosis in HeLa cells at the same time as H3pS10, indicating that co-responsibility for transition of G2/M phases. AURKB promotes the phosphorylation of histone H3 at pS10.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Histone H3 Antibody (NB21-1091) (0)

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Review for Histone H3 Antibody (NB21-1091) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: ICC.

Reviews using NB21-1091:
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Images Ratings Applications Species Date Details
Immunocytochemistry Histone H3 NB21-1091
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reviewed by:
Anony- mous
ICC Human 07/01/2014
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Summary

ApplicationImmunocytochemistry
SpeciesHuman
Special ApplicationsIF Confocal analysis of HeLa cells using Histone H3 [p Ser10] antibody (NB21-1091, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).
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Product General Protocols

View specific protocols for Histone H3 Antibody (NB21-1091): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Histone H3 Antibody (NB21-1091) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB21-1091AF405
Alexa Fluor 488 Labeled NB21-1091AF488
Alexa Fluor 647 Labeled NB21-1091AF647
Alexa Fluor 700 Labeled NB21-1091AF700
Biotin Labeled NB21-1091B
DyLight 405 Labeled NB21-1091V
DyLight 488 Labeled NB21-1091G
DyLight 550 Labeled NB21-1091R
DyLight 650 Labeled NB21-1091C
FITC Labeled NB21-1091F
HRP Labeled NB21-1091H

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Bioinformatics Tool for Histone H3 Antibody (NB21-1091)

Discover related pathways, diseases and genes to Histone H3 Antibody (NB21-1091). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on Histone H3.

Article Review: Glucose-induced transcriptional regulation in cancer
Epigenetic mechanisms have been implicated in many physiological and pathophysiological processes. Among these, histone modifications including methylation, phosphorylation, acetylation and ubiquitination, significantly modify gene expression. In c...  Read full blog post.

The role of DNMT3B in the co-incidence of methyltransferase and tumor suppressor expression in malignancies
Epigenetics is the process of heritable change in gene activity despite alteration of the hosts DNA sequence, essentially causing a change in a phenotype without a change in the genotype of a host. To change the gene sequence without interfering w...  Read full blog post.

The role of DNMT3A in development
Epigenetics is the study of heritable change in gene activity despite alteration of the hosts DNA sequence.  Change in gene activity done independently of the DNA sequence is achieved by way of histone and DNA methylation.  Gene silencing in DNA ...  Read full blog post.

EZH1 has more to offer than gene repression
EZH1 is part of the Polycomb-group family of proteins, which are responsible for remodeling chromatin in genes and modulating epigenetic silencing during development.  Specifically, EZHI is a component of PRC2, or polycomb repressive complex-2.  PR...  Read full blog post.

Understanding Transcription with RNA Polymerase II
RNA polymerase II is a large 12-subunit complex that synthesizes all mRNAs and several non-coding RNAs in eukaryotic cells. It is a DNA-dependent RNA polymerase enzyme that catalyzes transcription of DNA into RNA based on the four ribonucleoside triph...  Read full blog post.

Histone H3
Eukaryotic chromosomes are formed through the highly organized and structural wrapping of DNA genetic material around histone proteins into the classic "bead on a string" globular structure of nucleosomes. The histone family consists of five family me...  Read full blog post.

EZH2: Epigenetic Regulation Made Easy!
Enhancer of Zeste homolog 2 (EZH2) is the methyltransferase enzyme responsible for trimethylating lysine 27 on histone H3 to produce H3K27Me3. EZH2 is a polycomb group protein that is an essential epigenetic regulator that is often found deregulated i...  Read full blog post.

Understanding the Reasons for Histone H3 K4 Trimethylation (H3K4Me3)
Epigenetic mechanisms allow distinction between the active and inactive compartments of the genome, allowing proper cell lineage and embryogenesis. The trimethylation of Histone 3 at lysine 4 (H3K4Me3) is a common epigenetic histone modification that...  Read full blog post.

The 'epi-genie' is Out of the Bottle: Functional Histone 3 Variants in Human Disease
Discovery of histone variants using highly specific antibodies has led to the emerging notion that alterations in histone modifications and further changes in chromatin structure are induced by exchange of histone variants. Covalent histone modificati...  Read full blog post.

"Come Fly with Me" - New Drosophila Model Developed for Direct in Vivo Study of Histones
Forming the major protein component of chromatin, histones are essential to the structure and organization of chromosomes, forming the nucleosome around which DNA is packaged and wrapped.Antibody studies have revealed histones undergo various posttr...  Read full blog post.

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Anony- mous
07/01/2014
Application: ICC
Species: Human

Bioinformatics

Gene Symbol HIST2H3C
Entrez
Uniprot