Immunocytochemistry/ Immunofluorescence: Histone H3 [p Ser10] Antibody [NB21-1091] - Immunofluorescence: [NB21-1091] - IF Confocal analysis of HeLa cells using Histone H3 [p Ser10] antibody (NB21-1091, 1:5). An Alexa ...read more
Dot Blot: Histone H3 [p Ser10] Antibody [NB21-1091] - Dot blot analysis of HIstone H3pS10 with picomoles of peptide.
Western Blot: Histone H3 [p Ser10] Antibody [NB21-1091] - WB analysis of Histone H3pS10 in HeLa histone prep lysates.
Immunocytochemistry/ Immunofluorescence: Histone H3 [p Ser10] Antibody [NB21-1091] - Immunofluorescence: [NB21-1091] - Histone H3pS10 antibody was tested in HeLa cells with FITC (green). Nuclei and actin were ...read more
Immunohistochemistry-Paraffin: Histone H3 [p Ser10] Antibody [NB21-1091] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human testis using Histone H3 [pS10] antibody at 1:500 on a Bond Rx autostainer (Leica ...read more
Western Blot: Histone H3 [p Ser10] Antibody [NB21-1091] - WB analysis of H3pS10 in C. elegans embryo lysate.
Human, mouse, and C. elegans. Predicted to react with many species including chicken, Xenopus, Drosophila, and plant based on 100% sequence homology.
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS and 30% Glycerol
0.05% Sodium Azide
Immunogen affinity purified
Epi-Plus antibody production in collaboration with Rockland Immunochemicals Inc.
Alternate Names for Histone H3 [p Ser10] Antibody
H3 histone, family 3A
H3 pS10 is typically associated with activation of transcription of genes associated with histone H3. This modification is linked to the initiation of chromatin condensation in G(2). During mitosis, H3pS10 is required for proper chromosome segregation. Formation of the H3pS10 modification seems to be regulated in part by p53, which interacts with histone modifying complexes. In development of the retina, the presence of H3pS10 is linked to the abundance of eye defects. This developmental effect of H3pS10 seems to be related to Psf2 and GPR84. The human Timeless protein (Tim) regulates the global H3pS10 phosphorylation in G2/M phase. VASP concentrations peak during mitosis in HeLa cells at the same time as H3pS10, indicating that co-responsibility for transition of G2/M phases. AURKB promotes the phosphorylation of histone H3 at pS10.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
IF Confocal analysis of HeLa cells using Histone H3 [p Ser10] antibody (NB21-1091, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).
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EZH2: Epigenetic Regulation Made Easy! Enhancer of Zeste homolog 2 (EZH2) is the methyltransferase enzyme responsible for trimethylating lysine 27 on histone H3 to produce H3K27Me3. EZH2 is a polycomb group protein that is an essential epigenetic regulator that is often found deregulated i... Read full blog post.
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