Western Blot: Histone H3 [ac Lys9] Antibody [NB21-1074] - Analysis of Histone H3 K9ac in HeLa histone prep.
Immunocytochemistry/ Immunofluorescence: Histone H3 [ac Lys9] Antibody [NB21-1074] - Histone H3.2 K9ac antibody was tested at 1:100 in HeLa cells with FITC (green).
Chromatin Immunoprecipitation: Histone H3 [ac Lys9] Antibody [NB21-1074] - 2 ug of NB21-1074 was used to IP DNA from fixed Hela cells alongside a no antibody (No Ab) control. DNA was measured by qRT-PCR and normalized ...read more
Western Blot: Histone H3 [ac Lys9] Antibody [NB21-1074] - Analysis of Histone H3 K9ac in NIH/3T3 histone preps.
Western Blot: Histone H3 [ac Lys9] Antibody [NB21-1074] - Analysis of H3K9ac in C. elegans embryo lysate.
Dot Blot: Histone H3 [ac Lys9] Antibody [NB21-1074] - Analysis of Histone H3 K9 acetyl in picomoles of peptide.
Rat reactivity reported in scientific literature (PMID: 28174211). Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant (Nicotiana tabacum, Zea mays, Triticum aestivum, and Encephalartos caffer) based on 100% sequence homology.
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS and 30% Glycerol
0.05% Sodium Azide
Immunogen affinity purified
Epi-Plus antibody production in collaboration with Rockland Immunochemicals Inc.
Alternate Names for Histone H3 [ac Lys9] Antibody
H3 histone, family 3A
Acetylation of histone H3 at the K9 residue is associated with chromosome condensation in mitotic cells. The presence of H3K9ac is correlated to H3K4 trimethylation, and their distribution is related to developmentally repressed genes in some species. It has been shown that neuron depolarization promotes intragenic histone acetylation leading toward the formation of this modification. In pituitary, dopamine receptor agonists lead toward apoptosis. However, in tumors, decreased availability and activity of these agonists, combined with decreased H3K9ac leads toward resistance to chemotherapies and cancer survival and proliferation. Nuclear receptor PPAR ' induces the enrichment of H3K9ac enrichment on downstream promoters, which has effects on transcription and ultimately nuclear receptor transactivation.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
The role of DNMT3A in development Epigenetics is the study of heritable change in gene activity despite alteration of the hosts DNA sequence. Change in gene activity done independently of the DNA sequence is achieved by way of histone and DNA methylation. Gene silencing in DNA ... Read full blog post.
Histone H3 Eukaryotic chromosomes are formed through the highly organized and structural wrapping of DNA genetic material around histone proteins into the classic "bead on a string" globular structure of nucleosomes. The histone family consists of five family me... Read full blog post.
EZH2: Epigenetic Regulation Made Easy! Enhancer of Zeste homolog 2 (EZH2) is the methyltransferase enzyme responsible for trimethylating lysine 27 on histone H3 to produce H3K27Me3. EZH2 is a polycomb group protein that is an essential epigenetic regulator that is often found deregulated i... Read full blog post.
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