Histone H3 [ac Lys9] Antibody

Images

 
Western Blot: Histone H3 [ac Lys9] Antibody [NB21-1074] - Histone H3 [ac Lys9] Antibody [NB21-1074] - WB analysis of Histone H3 K9ac in HeLa histone prep.
Immunocytochemistry: Histone H3 [ac Lys9] Antibody [NB21-1074] - Immunofluorescence: Histone H3 [ac Lys9] Antibody [NB21-1074] - Histone H3.2 K9ac antibody was tested at 1:100 in HeLa cells with FITC (green).
Chromatin Immunoprecipitation: Histone H3 [ac Lys9] Antibody [NB21-1074] - Histone H3 [ac Lys9] Antibody [NB21-1074] - 2 ug of NB21-1074 was used to IP DNA from fixed Hela cells alongside a no antibody (No Ab) control. ...read more
Western Blot: Histone H3 [ac Lys9] Antibody [NB21-1074] - Histone H3 [ac Lys9] Antibody [NB21-1074] - WB analysis of Histone H3 K9ac in NIH/3T3 histone preps.
Western Blot: Histone H3 [ac Lys9] Antibody [NB21-1074] - Histone H3 [ac Lys9] Antibody [NB21-1074] - WB analysis of H3K9ac in C. elegans embryo lysate.
Dot Blot: Histone H3 [ac Lys9] Antibody [NB21-1074] - Histone H3 [ac Lys9] Antibody [NB21-1074] - Dot blot analysis of Histone H3 K9 acetyl in picomoles of peptide.
Histone H3 [ac Lys9] Antibody [NB21-1074]

Product Details

Summary
Reactivity Hu, Mu, Rt, Ce, Ch, Dr, Pl, XpSpecies Glossary
Applications WB, ChIP, DB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.68 mg/ml

Histone H3 [ac Lys9] Antibody Summary

Immunogen
Synthetic acetylated peptide surrounding Lysine 9 of human Histone H3.2 [Swiss Prot Q71DI3].
Modification
ac Lys9
Localization
Nucleus. Chromosome.
Predicted Species
Plant (100%), Chicken (100%), Xenopus (100%), Drosophila (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HIST2H3C
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • Chromatin Immunoprecipitation 2-5 ug per million cells
  • Dot Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:100
Application Notes
This H3 K9 acetyl antibody is useful for ICC/IF, ChIP, Dot Blot, and Western Blot where a band is seen ~15 kDa in HeLa histone prep, NIH 3T3 histone prep, and C. elegans embryo lysate.
Positive Control
HeLa Lysate (NBP1-78155)
NIH 3T3 Lysate (NBP1-78157)
Publications
Read Publication using
NB21-1074 in the following applications:

  • WB
    1 publication

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 28174211). Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant (Nicotiana tabacum, Zea mays, Triticum aestivum, and Encephalartos caffer) based on 100% sequence homology.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
0.68 mg/ml
Purity
Immunogen affinity purified

Notes

Epi-Plus antibody production in collaboration with Rockland Immunochemicals Inc.

Alternate Names for Histone H3 [ac Lys9] Antibody

  • H3 histone, family 3A
  • H3.3AH3F3H3F3B
  • H3.3B
  • H3F3A
  • H3K9ac
  • Histone H3
  • histone H3.3
  • MGC87782
  • MGC87783

Background

Acetylation of histone H3 at the K9 residue is associated with chromosome condensation in mitotic cells. The presence of H3K9ac is correlated to H3K4 trimethylation, and their distribution is related to developmentally repressed genes in some species. It has been shown that neuron depolarization promotes intragenic histone acetylation leading toward the formation of this modification. In pituitary, dopamine receptor agonists lead toward apoptosis. However, in tumors, decreased availability and activity of these agonists, combined with decreased H3K9ac leads toward resistance to chemotherapies and cancer survival and proliferation. Nuclear receptor PPAR ' induces the enrichment of H3K9ac enrichment on downstream promoters, which has effects on transcription and ultimately nuclear receptor transactivation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Histone H3 Antibody (NB21-1074)(1)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 1 application: WB.


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Product General Protocols

View specific protocols for Histone H3 Antibody (NB21-1074): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for Histone H3 Antibody (NB21-1074) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB21-1074AF405
Alexa Fluor 488 Labeled NB21-1074AF488
Alexa Fluor 647 Labeled NB21-1074AF647
Alexa Fluor 700 Labeled NB21-1074AF700
Biotin Labeled NB21-1074B
DyLight 405 Labeled NB21-1074V
DyLight 488 Labeled NB21-1074G
DyLight 550 Labeled NB21-1074R
DyLight 650 Labeled NB21-1074C
FITC Labeled NB21-1074F
HRP Labeled NB21-1074H

Additional Histone H3 Products

Bioinformatics Tool for Histone H3 Antibody (NB21-1074)

Discover related pathways, diseases and genes to Histone H3 Antibody (NB21-1074). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on Histone H3.

Article Review: Glucose-induced transcriptional regulation in cancer
Epigenetic mechanisms have been implicated in many physiological and pathophysiological processes. Among these, histone modifications including methylation, phosphorylation, acetylation and ubiquitination, significantly modify gene expression. In c...  Read full blog post.

The role of DNMT3B in the co-incidence of methyltransferase and tumor suppressor expression in malignancies
Epigenetics is the process of heritable change in gene activity despite alteration of the hosts DNA sequence, essentially causing a change in a phenotype without a change in the genotype of a host. To change the gene sequence without interfering w...  Read full blog post.

The role of DNMT3A in development
Epigenetics is the study of heritable change in gene activity despite alteration of the hosts DNA sequence.  Change in gene activity done independently of the DNA sequence is achieved by way of histone and DNA methylation.  Gene silencing in DNA ...  Read full blog post.

EZH1 has more to offer than gene repression
EZH1 is part of the Polycomb-group family of proteins, which are responsible for remodeling chromatin in genes and modulating epigenetic silencing during development.  Specifically, EZHI is a component of PRC2, or polycomb repressive complex-2.  PR...  Read full blog post.

Understanding Transcription with RNA Polymerase II
RNA polymerase II is a large 12-subunit complex that synthesizes all mRNAs and several non-coding RNAs in eukaryotic cells. It is a DNA-dependent RNA polymerase enzyme that catalyzes transcription of DNA into RNA based on the four ribonucleoside triph...  Read full blog post.

Histone H3
Eukaryotic chromosomes are formed through the highly organized and structural wrapping of DNA genetic material around histone proteins into the classic "bead on a string" globular structure of nucleosomes. The histone family consists of five family me...  Read full blog post.

EZH2: Epigenetic Regulation Made Easy!
Enhancer of Zeste homolog 2 (EZH2) is the methyltransferase enzyme responsible for trimethylating lysine 27 on histone H3 to produce H3K27Me3. EZH2 is a polycomb group protein that is an essential epigenetic regulator that is often found deregulated i...  Read full blog post.

Understanding the Reasons for Histone H3 K4 Trimethylation (H3K4Me3)
Epigenetic mechanisms allow distinction between the active and inactive compartments of the genome, allowing proper cell lineage and embryogenesis. The trimethylation of Histone 3 at lysine 4 (H3K4Me3) is a common epigenetic histone modification that...  Read full blog post.

The 'epi-genie' is Out of the Bottle: Functional Histone 3 Variants in Human Disease
Discovery of histone variants using highly specific antibodies has led to the emerging notion that alterations in histone modifications and further changes in chromatin structure are induced by exchange of histone variants. Covalent histone modificati...  Read full blog post.

"Come Fly with Me" - New Drosophila Model Developed for Direct in Vivo Study of Histones
Forming the major protein component of chromatin, histones are essential to the structure and organization of chromosomes, forming the nucleosome around which DNA is packaged and wrapped.Antibody studies have revealed histones undergo various posttr...  Read full blog post.

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Bioinformatics

Gene Symbol HIST2H3C
Entrez
Uniprot