Histone H3 [ac Lys9] Antibody (AH3-120) - BSA Free

Western Blot: Histone H3 [ac Lys9] Antibody (AH3-120) [NB120-12179] - Histone fraction isolated from HeLa cells was separated on SDS-PAGE and probed with Monoclonal Anti-acetyl-Histone H3 (Ac-Lys9) Clone: AH3-120. The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate. Observed molecular weight is ~16 kDa

• Reactivity: Hu, Mu, Rt, Bv, Ce, Ch, Dr, Xp
• Applications: WB, ELISA, ICC/IF, MA
• Clone: AH3-120
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 2.1 mg/ml

Histone H3 [ac Lys9] Antibody (AH3-120) - BSA Free Summary

• Immunogen: This Histone H3 [ac Lys9] antibody (AH3-120) was raised against a synthetic, acetylated histone H3 peptide (amino acids 7-20, acetyl K9) corresponding to the N-terminus of human histone H3, conjugated to KLH.
• Modification: ac Lys9
• Localization: Nuclear
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: H3C14
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100 - 1:2000
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:2000
  • Microarray
  • Western Blot 1 - 2 ug/mL
• Application Notes: WB: Detects a band of approximately 17 kDa (determined using a whole cell extract of mouse fibroblasts 3T3 cell line treated with sodium butyrate). We advise you to centrifuge this product vial before use. ELISA: indirect. Staining of (acetyl K9) histone H3 in WB is specifically inhibited with the immunizing peptide. There is no inhibition with non-acetylated histone H3 peptide.
• Theoretical MW: 17 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Cross-reacts with Human. Expected to cross-react with Mouse, Rat, Xenopus laevis, Cow, a wide range of other species, C. elegans, Chicken and Drosophilia melanogaster due to very high or perfect sequence identity across many species. Not yet tested in other species. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 10 mM PBS (pH 7.4)
• Preservative: 15mM Sodium Azide
• Concentration: 2.1 mg/ml
• Purity: Protein A or G purified

Alternate Names for Histone H3 [ac Lys9] Antibody (AH3-120) - BSA Free

• H3 histone, family 3A
• H3
• H3.3A
• H3.3B
• H3F2
• H3F3
• H3F3A
• H3F3B
• H3FM
• H3K9ac
• HIST2H3C
• Histone H3
• histone H3.3
• MGC87782
• MGC87783

Background

Chromatin is an arrangement of DNA and proteins which form chromosomes. Chromatin is formed from nucleosomes, which are comprised of a set of four histone proteins (H2A, H2B, H3, H4) wrapped with DNA. Chromatin is very dynamic, where post-translational modifications can regulate DNA to be copied, transcribed, or repaired.

Histones are nuclear proteins responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Changes in chromatin structure play a large role in the regulation of transcription. The chromatin fibers are compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.

Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. Posttranslational modifications such as acetylation of core histones regulates gene expression, thus altering protein function and regulation (1). Histone H3 is primarily acetylated at lysines 9, 14, 18, and 23 and have a theoretical molecular weight of 15 kDa. Acetylation at lysine 9 and 14 appears to control histone deposition, chromatin assembly and active transcription. Methylation of arginine residues within histone H3 has also been linked to transcription regulation. Histone H3 has been linked to various types of cancer as a biomarker through the aberrant expression of histone deacetylase (HDAC) enzymes and changes to chromatins (2-4).

References

1. Zhang, Y. X., Akumuo, R. C., Espana, R. A., Yan, C. X., Gao, W. J., & Li, Y. C. (2018). The histone demethylase KDM6B in the medial prefrontal cortex epigenetically regulates cocaine reward memory. Neuropharmacology, 141, 113-125. doi:10.1016/j.neuropharm.2018.08.030

2. Nandakumar, V., Hansen, N., Glenn, H. L., Han, J. H., Helland, S., Hernandez, K, ...Meldrum, D. R. (2016). Vorinostat differentially alters 3D nuclear structure of cancer and non-cancerous esophageal cells. Sci Rep, 6, 30593. doi:10.1038/srep30593

3. Zhou, M., Li, Y., Lin, S., Chen, Y., Qian, Y., Zhao, Z., & Fan, H. (2019). H3K9me3, H3K36me3, and H4K20me3 Expression Correlates with Patient Outcome in Esophageal Squamous Cell Carcinoma as Epigenetic Markers. Dig Dis Sci, 64(8), 2147-2157. doi:10.1007/s10620-019-05529-2

4. Li, Y., Guo, D., Sun, R., Chen, P., Qian, Q., & Fan, H. (2019). Methylation Patterns of Lys9 and Lys27 on Histone H3 Correlate with Patient Outcome in Gastric Cancer. Dig Dis Sci, 64(2), 439-446. doi:10.1007/s10620-018-5341-8

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Histone H3 Antibody (NB120-12179)(1)

Publication using NB120-12179

• Halbert D, Domenyuk V, Spetzler D et al. Aptamers and uses thereof United States Patent Application US 9958448 B2 2018-01-01

Review for Histone H3 Antibody (NB120-12179) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 02/24/2020

Summary

• Application: Western Blot
• Sample Tested: 293T whole cell lysate
• Species: Human
• Lot: 093M4834

Bioinformatics

• Gene Symbol: H3C14
• Entrez:
  • 8290(Human)
  • 15078(Mouse)
  • 287352(Rat)