Histone H2AX [p Ser139] Antibody

Images

 
Immunohistochemistry: Histone H2AX [p Ser139] Antibody [NB100-2280] - Biological and molecular analysis of cutaneous wound healing in p73+/+ and p73-/- mice. Representative micrographs of immunohistochemistry (IHC) ...read more
Simple Western: Histone H2AX [p Ser139] Antibody [NB100-2280] - Simple Western lane view shows a specific band for Histone H2AX [p Ser139] in 0.2 mg/ml of Jurkat lysate(s). This experiment was performed under reducing ...read more
Immunohistochemistry: Histone H2AX [p Ser139] Antibody [NB100-2280] - Telomere dysfunctional induced foci (TIF) in HBV-related multistep hepatocarcinogenesis and the correlations thereof with stathmin and elongation ...read more
Immunohistochemistry-Paraffin: Histone H2AX [p Ser139] Antibody [NB100-2280] - Detection of human Histone H2AX [p Ser139] antibody by immunohistochemistry. Sample: FFPE section of human seminoma. Antibodies: Affinity ...read more
Immunohistochemistry: Histone H2AX [p Ser139] Antibody [NB100-2280] - Detection of Mouse Histone H2AX [p Ser139] by Immunohistochemistry. Sample: FFPE section of mouse colon carcinoma CT26. Antibodies: Affinity ...read more
Simple Western: Histone H2AX [p Ser139] Antibody [NB100-2280] - Electropherogram image(s) of corresponding Simple Western lane view. Histone H2AX [p Ser139] antibody was used at 1:20 dilution on Jurkat lysate(s).

Product Details

Summary
Reactivity Hu, Mu, Ca, Po, Bt, Bv, ChHa, Gp, Pm, RbSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.1 mg/ml

Order Details

Histone H2AX [p Ser139] Antibody Summary

Immunogen
This Histone H2AX [p Ser139] Antibody was developed against a synthetic phospho-peptide, which represented a portion of the C-terminus of human histone H2AX surrounding phosphorylated serine 139 (GeneID 3014).
Modification
p Ser139
Marker
DNA Double-strand break marker
Predicted Species
Porcine (100%), Rabbit (100%), Guinea Pig (100%), Bovine (100%), Bat (100%), Monkey (100%), Chinese Hamster (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
H2AX
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:100 - 1:500
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Simple Western 1:20
  • Western Blot 1:100-1:2000
Application Notes
Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining. Likely to work with frozen sections. Use in WB reported in scientific literature ( PMID 24415760). Use in IHC-Frozen reported in scientific literature (PMID 26577699).

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Theoretical MW
15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Histone H2AX Overexpression Lysate
Reviewed Applications
Read 1 Review rated 5
using
NB100-2280 in the following applications:

Publications
Read Publications using
NB100-2280 in the following applications:

Reactivity Notes

Based on sequence percent identity: Gorilla (100%), Macaque (100%), Canine reactivity reported in scientific literature (PMID: 26991424).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.1 mg/ml
Purity
Immunogen affinity purified

Notes

Licensed to Novus Biologicals LLC under U.S. Patent Nos. 6,362,317 and 6,884,873.

Alternate Names for Histone H2AX [p Ser139] Antibody

  • H2A.X
  • H2A/X
  • H2AFX
  • Histone H2AX

Background

Gamma H2AX (gammaH2AX) is the phosphorylated version of histone H2AX and is a marker for double-stranded breaks (DSBs) caused by DNA damage (1-4). H2AX is a variant of histone H2A, one of the histone core molecules forming the nucleosome, and is a vital component in repairing DNA damage (1-4). The H2AX variant represents between 2-25% of total H2A (1-3). Human H2AX protein is 143 amino acids in length and has a theoretical molecular weight of 15 kDa (5). As a result of DSBs, H2AX becomes phosphorylated at serine-139 and gamma H2AX is generated as a response to genomic instability (1-4). Gamma H2AX and its phosphorylation can be detected through western blotting and immunofluorescence, serving as a biomarker for DSB frequency (1-4). Ataxia telangiectasia mutated (ATM) is considered a key protein for H2AX phosphorylation (1-4). Upon the phosphorylation and formation of gamma H2AX, many repair proteins become associated including breast cancer 1 protein (BRCA1), NBS1 (Nijmegen breakage syndrome 1)/hMRE11 (human meiotic recombination 11 protein)/ hRAD50 (N/M/R) repair complex, and 53BP1 (p53 binding protein 1) (1-4). Gamma H2AX detection assays are currently utilized for a variety of purposes, from studying DNA repair to drug development and radiation biodosimetry (4).

References

1. Palla, V. V., Karaolanis, G., Katafigiotis, I., Anastasiou, I., Patapis, P., Dimitroulis, D., & Perrea, D. (2017). gamma-H2AX: Can it be established as a classical cancer prognostic factor?. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine. https://doi.org/10.1177/1010428317695931

2. Kuo, L. J., & Yang, L. X. (2008). Gamma-H2AX - a novel biomarker for DNA double-strand breaks. In vivo (Athens, Greece).

3. Kinner, A., Wu, W., Staudt, C., & Iliakis, G. (2008). Gamma-H2AX in recognition and signaling of DNA double-strand breaks in the context of chromatin. Nucleic acids research. https://doi.org/10.1093/nar/gkn550

4. Redon, C. E., Weyemi, U., Parekh, P. R., Huang, D., Burrell, A. S., & Bonner, W. M. (2012). gamma-H2AX and other histone post-translational modifications in the clinic. Biochimica et biophysica acta. https://doi.org/10.1016/j.bbagrm.2012.02.021

5. H2AX: Uniprot (P16104)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Histone H2AX Antibody (NB100-2280)(32)

We have publications tested in 3 confirmed species: Human, Mouse, Canine.

We have publications tested in 5 applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB.


Filter By Application
ICC/IF
(8)
IHC
(4)
IHC-Fr
(1)
IHC-P
(9)
WB
(5)
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Human
(10)
Mouse
(13)
Canine
(1)
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Showing Publications 1 - 10 of 32. Show All 32 Publications.
Publications using NB100-2280 Applications Species
Marquez-Exposito L, Tejedor-Santamaria L, Valentijn FA et al. Oxidative Stress and Cellular Senescence Are Involved in the Aging Kidney Antioxidants (Basel, Switzerland) Jan 31 2022 [PMID: 35204184] (IHC-P, Mouse) IHC-P Mouse
Zhou Z, Yin H, Suye S et al. The changes of DNA double-strand breaks and DNA repair during ovarian reserve formation in mice Reproductive biology Jan 10 2022 [PMID: 35026551] (IHC, Mouse) IHC Mouse
Valentijn FA, Knoppert SN, Pissas G et al. CCN2 Aggravates the Immediate Oxidative Stress-DNA Damage Response following Renal Ischemia-Reperfusion Injury Antioxidants (Basel, Switzerland) Dec 20 2021 [PMID: 34943123] (IHC-P, Mouse) IHC-P Mouse
Ruggiano A, Vaz B, Kilgas S et al. The protease SPRTN and SUMOylation coordinate DNA-protein crosslink repair to prevent genome instability Cell reports Dec 7 2021 [PMID: 34879279]
Jardim-Perassi BV, Mu W, Huang S et al. Deep-learning and MR images to target hypoxic habitats with evofosfamide in preclinical models of sarcoma Theranostics Mar 11 2021 [PMID: 33859749] (IHC, Mouse) IHC Mouse
Grgic I, Tschanz F, Bosrgeaud N et al. Tumor Oxygenation by Myo-Inositol Trispyrophosphate Enhances Radiation Response International journal of radiation oncology, biology, physics Feb 12 2021 [PMID: 33587991]
Low HY, Lee YC, Lee YJ et al. Reciprocal Regulation Between Indoleamine 2,3-Dioxigenase 1 and Notch1 Involved in Radiation Response of Cervical Cancer Stem Cells Cancers (Basel) Jun 12 2020 [PMID: 32545442] (IHC, Human) IHC Human
Han J, Wang H, Zhang T et al. Resveratrol attenuates doxorubicin-induced meiotic failure through inhibiting oxidative stress and apoptosis in mouse oocytes Aging (Albany NY) Apr 30 2020 [PMID: 32352929] (ICC/IF, Mouse) ICC/IF Mouse
Pokrovska T D, Jacobus E J et al. External Beam Radiation Therapy and Enadenotucirev: Inhibition of the DDR and Mechanisms of Radiation-Mediated Virus Increase. Cancers (Basel) Mar 26 2020 [PMID: 32224979] (ICC/IF, Human) ICC/IF Human
Ma H, Yang F, Ding XQ Inhibition of thyroid hormone signaling protects retinal pigment epithelium and photoreceptors from cell death in a mouse model of age-related macular degeneration Cell Death Dis Jan 13 2020 [PMID: 31932580] (ICC/IF, Mouse) ICC/IF Mouse
Show All 32 Publications.

Review for Histone H2AX Antibody (NB100-2280) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-2280:
Filter by Applications
IHC-P
(1)
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Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin Histone H2AX NB100-2280
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5
reviewed by:
Emily Beirne
IHC-P Human 10/06/2015
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedRamos lymphoma
SpeciesHuman
Lot4

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  1. Could you direct me where to find secondary antibodies for NB100-2280 that would be appropriate for certain applications?
    • By clicking on the "Support & Research" tab on NB100-2280's product page under the "Product Details" display, you will find a link to view all anti-Rabbit secondary antibodies. This will lead you to a new page that will allow you to filter products by application.

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Recent Reviews

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Emily Beirne
10/06/2015
Application: IHC-P
Species: Human

Bioinformatics

Gene Symbol H2AX
Entrez
Uniprot