Histone H2AX [p Ser139] Antibody

Immunohistochemistry: Histone H2AX [p Ser139] Antibody [NB100-2280] - Biological and molecular analysis of cutaneous wound healing in p73+/+ and p73-/- mice. Representative micrographs of immunohistochemistry (IHC) staining for Histone H2AX [p Ser139] in skin specimens from p73+/+ and p73-/- mice 10 days after wounding. All scale bars represent 50 um. Regions of the skin are labeled as: IFE, HF, epidermal wound edge (WE), and newly-formed epidermis of the wound (W); and the dotted line indicates the border between the WE and W. *p-value < 0.05, **p-value < 0.01, ***p-value < 0.001. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0218458), licensed under a CC-BY license.

Simple Western: Histone H2AX [p Ser139] Antibody [NB100-2280] - Simple Western lane view shows a specific band for Histone H2AX [p Ser139] in 0.2 mg/ml of Jurkat lysate(s). This experiment was performed under reducing conditions using the 12 - 230 kDa separation system.

Immunohistochemistry: Histone H2AX [p Ser139] Antibody [NB100-2280] - Telomere dysfunctional induced foci (TIF) in HBV-related multistep hepatocarcinogenesis and the correlations thereof with stathmin and elongation factor 1alpha (EF1alpha) expression. A. Representative features of colocalization of Histone H2AX [p Ser139] and telomeric DNA in defined lesions of human multistep hepatocarcinogenesis. TIF are indicated by colored arrowheads: blue, DAPI; green, gamma H2AX; red, telomeres; yellow, TIF. Image collected and cropped by CiteAb from the following publication (//translational-medicine.biomedcentral.com/articles/10.1186/1479-5876-12-154) licensed under a CC-BY license.

Immunohistochemistry-Paraffin: Histone H2AX [p Ser139] Antibody [NB100-2280] - Detection of human Histone H2AX [p Ser139] antibody by immunohistochemistry. Sample: FFPE section of human seminoma. Antibodies: Affinity purified rabbit Histone H2AX [p Ser139] antibody used at a dilution of 1:500. Detection: DAB.

Immunohistochemistry: Histone H2AX [p Ser139] Antibody [NB100-2280] - Detection of Mouse Histone H2AX [p Ser139] by Immunohistochemistry. Sample: FFPE section of mouse colon carcinoma CT26. Antibodies: Affinity purified rabbit Histone H2AX [p Ser139] antibody. Detection: DAB.

Simple Western: Histone H2AX [p Ser139] Antibody [NB100-2280] - Electropherogram image(s) of corresponding Simple Western lane view. Histone H2AX [p Ser139] antibody was used at 1:20 dilution on Jurkat lysate(s).

• Reactivity: Hu, Mu, Ca, Po, Bt, Bv, ChHa, Gp, Pm, Rb
• Applications: WB, Simple Western, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.1 mg/ml

Histone H2AX [p Ser139] Antibody Summary

• Immunogen: This Histone H2AX [p Ser139] Antibody was developed against a synthetic phospho-peptide, which represented a portion of the C-terminus of human histone H2AX surrounding phosphorylated serine 139 (GeneID 3014).
• Modification: p Ser139
• Marker: DNA Double-strand break marker
• Predicted Species: Porcine (100%), Rabbit (100%), Guinea Pig (100%), Bovine (100%), Bat (100%), Monkey (100%), Chinese Hamster (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: H2AX
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:100 - 1:500
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Simple Western 1:20
  • Western Blot 1:100-1:2000
• Application Notes: Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining. Likely to work with frozen sections. Use in WB reported in scientific literature ( PMID 24415760). Use in IHC-Frozen reported in scientific literature (PMID 26577699).
  
  In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
• Theoretical MW: 15 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Histone H2AX Overexpression Lysate

Reactivity Notes

Based on sequence percent identity: Gorilla (100%), Macaque (100%), Canine reactivity reported in scientific literature (PMID: 26991424).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.1 mg/ml
• Purity: Immunogen affinity purified

Notes

Licensed to Novus Biologicals LLC under U.S. Patent Nos. 6,362,317 and 6,884,873.

Alternate Names for Histone H2AX [p Ser139] Antibody

• H2A.X
• H2A/X
• H2AFX
• Histone H2AX

Background

Gamma H2AX (gammaH2AX) is the phosphorylated version of histone H2AX and is a marker for double-stranded breaks (DSBs) caused by DNA damage (1-4). H2AX is a variant of histone H2A, one of the histone core molecules forming the nucleosome, and is a vital component in repairing DNA damage (1-4). The H2AX variant represents between 2-25% of total H2A (1-3). Human H2AX protein is 143 amino acids in length and has a theoretical molecular weight of 15 kDa (5). As a result of DSBs, H2AX becomes phosphorylated at serine-139 and gamma H2AX is generated as a response to genomic instability (1-4). Gamma H2AX and its phosphorylation can be detected through western blotting and immunofluorescence, serving as a biomarker for DSB frequency (1-4). Ataxia telangiectasia mutated (ATM) is considered a key protein for H2AX phosphorylation (1-4). Upon the phosphorylation and formation of gamma H2AX, many repair proteins become associated including breast cancer 1 protein (BRCA1), NBS1 (Nijmegen breakage syndrome 1)/hMRE11 (human meiotic recombination 11 protein)/ hRAD50 (N/M/R) repair complex, and 53BP1 (p53 binding protein 1) (1-4). Gamma H2AX detection assays are currently utilized for a variety of purposes, from studying DNA repair to drug development and radiation biodosimetry (4).

References

1. Palla, V. V., Karaolanis, G., Katafigiotis, I., Anastasiou, I., Patapis, P., Dimitroulis, D., & Perrea, D. (2017). gamma-H2AX: Can it be established as a classical cancer prognostic factor?. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine. https://doi.org/10.1177/1010428317695931

2. Kuo, L. J., & Yang, L. X. (2008). Gamma-H2AX - a novel biomarker for DNA double-strand breaks. In vivo (Athens, Greece).

3. Kinner, A., Wu, W., Staudt, C., & Iliakis, G. (2008). Gamma-H2AX in recognition and signaling of DNA double-strand breaks in the context of chromatin. Nucleic acids research. https://doi.org/10.1093/nar/gkn550

4. Redon, C. E., Weyemi, U., Parekh, P. R., Huang, D., Burrell, A. S., & Bonner, W. M. (2012). gamma-H2AX and other histone post-translational modifications in the clinic. Biochimica et biophysica acta. https://doi.org/10.1016/j.bbagrm.2012.02.021

5. H2AX: Uniprot (P16104)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Histone H2AX Antibody (NB100-2280)(42)

Publications using NB100-2280

• Suye S, Yin H, Zhou Z et al. Histological and transcriptomic analysis of Fance-deficient PGCs reveal the possible mechanisms of their depletion Reproduction (Cambridge, England) 2023-07-01 [PMID: 37184052]
• Eleftheriadis T, Pissas G, Filippidis G et al. Dapagliflozin Prevents High-Glucose-Induced Cellular Senescence in Renal Tubular Epithelial Cells International journal of molecular sciences 2022-12-17 [PMID: 36555751] (WB, Human)
  
  Details:
  Dilution used in WB 1:1000
• Beeler JSA The Role of p73 in Basal Keratinocyte Function Thesis (ICC/IF, Mouse)
• Lopez KA Therapeutic targeting of histone H3 lysine 36 trimethylation-deficient cancers Thesis 2019-01-01 (ICC/IF, Human)
• Ma H, Yang F, York LR et al. Excessive Thyroid Hormone Signaling Induces Photoreceptor Degeneration in Mice eNeuro 2023-08-18 [PMID: 37596046] (ICC/IF, WB)
• Eleftheriadis T, Pissas G, Golfinopoulos S et al. Routes of Albumin Overload Toxicity in Renal Tubular Epithelial Cells International journal of molecular sciences 2023-06-01 [PMID: 37298591] (WB, IHC-P, Mouse)
• Sun M, Wang T, Zhou Y et al. Pulmonary flora-modified diesel particulate matter induced lung injury via cGAS signaling pathway The Science of the total environment 2023-05-29 [PMID: 37257608]
• Si B, Wang X, Liu Y et al. Multi-locus deletion mutation induced by silver nanoparticles: Role of lysosomal-autophagy dysfunction Ecotoxicology and environmental safety 2023-04-25 [PMID: 37105094] (WB, Human)
• Rodriguez-Berriguete G, Ranzani M, Prevo R et al. Small-Molecule Pol? Inhibitors Provide Safe and Effective Tumor Radiosensitization in Preclinical Models Clinical Cancer Research 2023-04-14 [PMID: 36689546] (ICC/IF)
• Valentijn F, Knoppert S, Marquez-Exposito L et al. CCN2 aggravates acute DNA damage and the subsequent DDR-Senescence-Fibrosis sequence following renalischemia-reperfusion injury Kidney International 2022-01-01 [PMID: 35921911] (WB, IHC-P, IHC-P, WB, Human, Mouse)
  
  Details:
  Dilution used 1:1000
• Marquez-Exposito L, Tejedor-Santamaria L, Valentijn FA et al. Oxidative Stress and Cellular Senescence Are Involved in the Aging Kidney Antioxidants (Basel, Switzerland) 2022-01-31 [PMID: 35204184] (IHC-P, Mouse)
• Zhou Z, Yin H, Suye S et al. The changes of DNA double-strand breaks and DNA repair during ovarian reserve formation in mice Reproductive biology 2022-01-10 [PMID: 35026551] (IF/IHC, Mouse)
• Valentijn FA, Knoppert SN, Pissas G et al. CCN2 Aggravates the Immediate Oxidative Stress-DNA Damage Response following Renal Ischemia-Reperfusion Injury Antioxidants (Basel, Switzerland) 2021-12-20 [PMID: 34943123] (IHC-P, Mouse)
• Ruggiano A, Vaz B, Kilgas S et al. The protease SPRTN and SUMOylation coordinate DNA-protein crosslink repair to prevent genome instability Cell reports 2021-12-07 [PMID: 34879279]
• Marquez-Exposito L, Tejedor-Santamaria L, Santos-Sanchez L et al. Acute Kidney Injury is Aggravated in Aged Mice by the Exacerbation of Proinflammatory Processes Frontiers in Pharmacology 2021-06-22 [PMID: 34239439]
• Jardim-Perassi BV, Mu W, Huang S et al. Deep-learning and MR images to target hypoxic habitats with evofosfamide in preclinical models of sarcoma Theranostics 2021-03-11 [PMID: 33859749] (IF/IHC, Mouse)
• Grgic I, Tschanz F, Bosrgeaud N et al. Tumor Oxygenation by Myo-Inositol Trispyrophosphate Enhances Radiation Response International journal of radiation oncology, biology, physics 2021-02-12 [PMID: 33587991]
• Low HY, Lee YC, Lee YJ et al. Reciprocal Regulation Between Indoleamine 2,3-Dioxigenase 1 and Notch1 Involved in Radiation Response of Cervical Cancer Stem Cells Cancers (Basel) 2020-06-12 [PMID: 32545442] (IF/IHC, Human)
• Han J, Wang H, Zhang T et al. Resveratrol attenuates doxorubicin-induced meiotic failure through inhibiting oxidative stress and apoptosis in mouse oocytes Aging (Albany NY) 2020-04-30 [PMID: 32352929] (ICC/IF, Mouse)
• Pokrovska T D, Jacobus E J et al. External Beam Radiation Therapy and Enadenotucirev: Inhibition of the DDR and Mechanisms of Radiation-Mediated Virus Increase. Cancers (Basel) 2020-03-26 [PMID: 32224979] (ICC/IF, Human)
• Ma H, Yang F, Ding XQ Inhibition of thyroid hormone signaling protects retinal pigment epithelium and photoreceptors from cell death in a mouse model of age-related macular degeneration Cell Death Dis 2020-01-13 [PMID: 31932580] (ICC/IF, Mouse)
• Singh AN, Oehler J, Torrecilla I et al. The p97-Ataxin 3 complex regulates homeostasis of the DNA damage response E3 ubiquitin ligase RNF8 EMBO J. 2019-10-15 [PMID: 31613024] (WB, Human)
• Lu M, Hartmann D, Braren R et al. Oncogenic Akt-FOXO3 loop favors tumor-promoting modes and enhances oxidative damage-associated hepatocellular carcinogenesis BMC Cancer 2019-09-05 [PMID: 31488102] (IHC-P, Mouse)
• Burdova K, Yang H, Faedda R et al. E2F1 proteolysis via SCF-cyclin F underlies synthetic lethality between cyclin F loss and Chk1 inhibition EMBO J. 2019-08-19 [PMID: 31424118] (WB, Human)
• Beeler JS, Marshall CB, Gonzalez-Ericsson PI, et al. p73 regulates epidermal wound healing and induced keratinocyte programming PLoS ONE 2019-06-19 [PMID: 31216312] (IHC-P, Mouse)
• Wen J, Yan H, He M et al. GSK-3b protects fetal oocytes from premature death via modulating TAp63 expression in mice BMC Biol. 2019-03-12 [PMID: 30866939] (ICC/IF, Mouse)
• Cespedes MV, Guillen MJ, Lopez-Casas PP et al. Lurbinectedin induces depletion of tumor-associated macrophages, an essential component of its in vivo synergism with gemcitabine, in pancreatic adenocarcinoma mouse models. Dis Model Mech. 2016-12-01 [PMID: 27780828] (IF/IHC, Human)
• Jamal S, Cheriyan VT, Muthu M et al. Establishment and characterization of a canine soft tissue sarcoma patient-derived xenograft model. PLOS One [PMID: 26991424] (IHC-P, Canine)
• Franz A, Pirson PA, Pilger D et al. Chromatin-associated degradation is defined by UBXN-3/FAF1 to safeguard DNA replication fork progression. Nat Commun 2016-02-04 [PMID: 26842564] (WB)
• Li X Endoplasmic reticulum stress sensor ATF6 as an immunometabolic modulator in hepatic tumorigenesis Thesis 2023-01-01 (IHC-P, Mouse)
• Wang M, Topalovski M, Toombs JE et al. Fibulin-5 Blocks Microenvironmental ROS in Pancreatic Cancer. Cancer Res. 2015-11-17 [PMID: 26577699] (IHC-Fr, Mouse)
• Ahn EY, Yoo JE, Rhee H et al. Increased expression of stathmin and elongation factor 1alpha in precancerous nodules with telomere dysfunction in hepatitis B viral cirrhotic patients. J Transl Med. 2014-05-31 [PMID: 24885363] (IHC-P, Human)
• Yoo JE, Park YN, Oh BK. PinX1, a telomere-repeat-binding factor 1 (TRF1) interacting protein, maintains telomere integrity by modulating TRF1 homeostasis, the process in which human telomerase reverse transcriptase (hTERT) plays dual roles. J. Biol. Chem. 2014-01-10 [PMID: 24415760] (WB, ICC/IF, Human)
• Kreahling JM, Foroutan P, Reed D et al. Wee1 Inhibition by MK-1775 Leads to Tumor Inhibition and Enhances Efficacy of Gemcitabine in Human Sarcomas PLoS One 2013-01-01 [PMID: 23520471] (IHC-P, Human)
• Westendorp B, Mokry M, Groot Koerkamp MJA et al. E2F7 represses a network of oscillating cell cycle genes to control S-phase progression. Nucleic Acids Research. 2011-12-17 [PMID: 22180533]
• Mahalingam D, Tay LL, Tan WH, Chai JH, Wang X. Mutant telomerase RNAs induce DNA damage and apoptosis via the TRF2-ATM pathway in telomerase-overexpressing primary fibroblasts. FEBS J;278(19):3724-38. 2011-10-01 [PMID: 21824286] (ICC/IF, Human)
• Sperry M, Charrez B, Fotowat H et al. Identification of a pharmaceutical biostasis inducer that slows metabolism in multiple vertebrates that do not hibernate bioRxiv 2023-02-28 (ICC/IF)
• Pearson HB, McGlinn E, Phesse TJ et al. The polarity protein Scrib mediates epidermal development and exerts a tumor suppressive function during skin carcinogenesis. Mol. Cancer 2015-09-17 [PMID: 26376988] (IHC-P, Mouse)
• M K Schluterman, S L Chapman, G Korpanty et al. Loss of fibulin-5 binding to {beta}1 integrins inhibits tumor growth by increasing the level of ROS. Dis. Model. Mech. 3 (5-6): 333-342. 2010-01-01 [PMID: 20197418]
• Li et al. Cancer Res 70 (2):646-654. 2010-01-01 [PMID: 20068155]
• Stachelek et al. Cancer Res 70 (1):409-417. 2010-01-01 [PMID: 20028873]
• Konstantinidou et al. Cancer Res 69 (19):7644-7652. 2009-01-01 [PMID: 19789349]

Review for Histone H2AX Antibody (NB100-2280) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer IHC-P Human 10/06/2015

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Ramos lymphoma
• Species: Human
• Lot: 4

FAQs for Histone H2AX Antibody (NB100-2280). (Showing 1 - 1 of 1 FAQs).

1. Could you direct me where to find secondary antibodies for NB100-2280 that would be appropriate for certain applications?
   • By clicking on the "Support & Research" tab on NB100-2280's product page under the "Product Details" display, you will find a link to view all anti-Rabbit secondary antibodies. This will lead you to a new page that will allow you to filter products by application.

Bioinformatics

• Gene Symbol: H2AX
• Entrez:
  • 3014(Human)
  • 15270(Mouse)
• Uniprot:
  • P16104()