Histone Deacetylase 2/HDAC2 Antibody (806116)

Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, SH-SY5Y human neuroblastoma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human Histone Deacetylase 2/HDAC2 Monoclonal Antibody (Catalog # MAB7679) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Histone Deacetylase 2/HDAC2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western lane view shows lysates of K562 human chronic myelogenous leukemia cell line, loaded at 0.5 mg/mL. A specific band was detected for Histone Deacetylase 2/HDAC2 at approximately 98 kDa (as indicated) using 2 µg/mL of Mouse Anti-Human/Rat Histone Deacetylase 2/HDAC2 Monoclonal Antibody (Catalog # MAB7679). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reactivity: Hu, Rt
• Applications: WB, Simple Western
• Clone: 806116
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Histone Deacetylase 2/HDAC2 Antibody (806116) Summary

• Immunogen: E. coli-derived recombinant human Histone Deacetylase 2/HDAC-2
  Pro386-Pro488
  Accession # Q92769
• Specificity: Detects human Histone Deacetylase 2/HDAC-2 in direct ELISAs and human and rat Histone Deacetylase 2/HDAC-2 Western blots. In direct ELISAs, no cross-reactivity with recombinant human HDAC1 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: HDAC2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Simple Western 2 ug/mL
  • Western Blot 0.2 ug/mL
• Application Notes: In Simple Western only 10-15 uL of the recommended dilution is used per data point.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Histone Deacetylase 2/HDAC2 Antibody (806116)

• EC 3.5.1.98
• HD2
• HDAC2
• Histone Deacetylase 2
• RPD3
• transcriptional regulator homolog RPD3
• YAF1
• YY1-associated factor 1

Background

Histone deacetylase 2 (HDAC2), is a ubiquitously expressed intracellular enzyme that is involved in the post-translational modification of proteins. Acetyl groups are added to e-NH3 on lysine residues by acetyltransferases and removed by HDACs. The balance between these reactions has widespread effects on the enzymatic activity, subcellular localization, and molecular interactions of the substrates. HDAC2 can associate into several multiprotein complexes with discrete functions. Acetylation of core histones (H2A, H2B, H3, and H4) alters their interaction with DNA and the local availability of DNA for transcription. The function of HDAC2 itself is regulated by several post-translational modifications including phosphorylation, acetylation, ubiquitination, tyrosine nitrosylation, and cysteine carbonylation. Within aa 386-488, human HDAC2 shares 98% and 96% aa sequence identity with mouse and rat HDAC2, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Histone Deacetylase 2/HDAC2 Antibody (MAB7679)(2)

Publications using MAB7679

• Xue‑Jiao Sun, Zhan‑Hua Li, Yang Zhang, Xiao‑Ning Zhong, Zhi‑Yi He, Ji‑Hong Zhou et al. Theophylline and dexamethasone in combination reduce inflammation and prevent the decrease in HDAC2 expression seen in monocytes exposed to cigarette smoke extract Experimental and Therapeutic Medicine 2020-03-06 [PMID: 32269608] (Western Blot, Human)
• Bandolik JJ, Hamacher A, Schrenk C et al. Class I-Histone Deacetylase (HDAC) Inhibition is Superior to pan-HDAC Inhibition in Modulating Cisplatin Potency in High Grade Serous Ovarian Cancer Cell Lines International Journal of Molecular Sciences 6/22/2019 [PMID: 31234549]

Bioinformatics

• Gene Symbol: HDAC2
• Uniprot:
  • Q92769(Human)