HIST1H2BA Antibody (OTI1C9) - Azide and BSA Free

Western Blot: HIST1H2BA Antibody (OTI1C9) - Azide and BSA Free [NBP2-72479] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HIST1H2BA.

Immunohistochemistry: HIST1H2BA Antibody (OTI1C9) - Azide and BSA Free [NBP2-72479] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Immunohistochemistry: HIST1H2BA Antibody (OTI1C9) - Azide and BSA Free [NBP2-72479] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: OTI1C9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

HIST1H2BA Antibody (OTI1C9) - Azide and BSA Free Summary

• Immunogen: Human recombinant protein fragment corresponding to amino acids 2-127 of human HIST1H2BA(NP_733759) produced in E.coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: HIST1H2BA
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
• Theoretical MW: 14 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for HIST1H2BA Antibody (OTI1C9) - Azide and BSA Free

• histone H2B type 1-A
• bA317E16.3
• H2BFU
• histone cluster 1, H2ba
• STBP
• TSH2B

Background

HIST1H2BA is 127 amino acids long, weighs approximately 14 kDa, and the gene codes for a part of a group of basic nuclear proteins called histones, which are responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Current studies are being done on several diseases and disorders including systemic lupus erythematosus, lupus erythematosus, papillary adenoma, adenoma, and immunodeficiency. HIST1H2BA has also been shown to have interactions with BRD7, DNTTIP2, HIST2H2AC, SET, and CCNA1 in pathways such as the telomere maintenance, nucleosome assembly, cell cycle, FOXM1 transcription factor, and systemic lupus erythematosus pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HIST1H2BA