HIRA Antibody - BSA Free

Immunohistochemistry-Paraffin: HIRA Antibody [NBP2-38636] - Staining of human thyroid gland shows high expression.

Immunohistochemistry-Paraffin: HIRA Antibody [NBP2-38636] - Staining of human liver shows low expression as expected.

Immunohistochemistry-Paraffin: HIRA Antibody [NBP2-38636] - Staining in human thyroid gland and liver tissues using anti-HIRA antibody. Corresponding HIRA RNA-seq data are presented for the same tissues.

• Reactivity: Hu, Mu, Rt
• Applications: IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

HIRA Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit HIRA Antibody - BSA Free (NBP2-38636) is a polyclonal antibody validated for use in IHC. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against a recombinant protein corresponding to amino acids: LKLPIPSPQRAFTLQVSSDPSMYIEVENEVTVVGGVKLSRLKCNREGKEWETVLTSRILTAAGSCDVVCVACEKRMLSVFSTCGRRLLSPILLPSPISTLHC
• Predicted Species: Mouse (93%), Rat (93%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HIRA
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  HIRA Protein (NBP2-38636PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for HIRA Antibody - BSA Free

• DGCR1DiGeorge critical region gene 1
• HIR (histone cell cycle regulation defective) homolog A (S. cerevisiae)
• HIR histone cell cycle regulation defective homolog A (S. cerevisiae)
• HIR
• TUP1
• TUP1-like enhancer of split protein 1
• TUPLE1protein HIRA

Background

HIRA encodes a histone chaperone that preferentially places the variant histone H3.3 in nucleosomes. Orthologs of this gene in yeast, flies, and plants are necessary for the formation of transcriptionally silent heterochomatin. This gene plays an important role in the formation of the senescence-associated heterochromatin foci. These foci likely mediate the irreversible cell cycle changes that occur in senescent cells. It is considered the primary candidate gene in some haploinsufficiency syndromes such as DiGeorge syndrome, and insufficient production of the gene may disrupt normal embryonic development. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HIRA
• Uniprot:
  • P54198(Human)