HIPPI Antibody - BSA Free

Western Blot: HIPPI Antibody [NBP2-16835] - Sample (30 ug of whole cell lysate) A: Raji 10% SDS PAGE gel, diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: HIPPI Antibody [NBP2-16835] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.

Immunohistochemistry-Paraffin: HIPPI Antibody [NBP2-16835] - Immunohistochemical analysis of paraffin-embedded H661 xenograft, using antibody at 1:500 dilution.

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

HIPPI Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit HIPPI Antibody - BSA Free (NBP2-16835) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human HIPPI. The exact sequence is proprietary.
• Localization: Cell projection; cilium basal body (By similarity)
• Predicted Species: Mouse (91%), Rat (91%), Bovine (92%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IFT57
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 49 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Xenopus laevis (83%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for HIPPI Antibody - BSA Free

• Dermal papilla-derived protein 8
• DERP8
• ESRRBL1huntingtin interacting protein-1 interacting protein
• estrogen-related receptor beta like 1
• Estrogen-related receptor beta-like protein 1
• FLJ10147
• HIPPIHIP1-interacting protein
• intraflagellar transport 57 homolog (Chlamydomonas)
• intraflagellar transport protein 57 homolog
• MHS4R2HIP1 protein interactor

Background

Required for the formation of cilia. Plays an indirect role in sonic hedgehog signaling, cilia being required for all activity of the hedgehog pathway (By similarity). Has pro-apoptotic function via its interaction with HIP1, leading to recruit caspase-8 (CASP8) and trigger apoptosis. Has the ability to bind DNA sequence motif 5'-AAAGACATG-3' present in the promoter of caspase genes such as CASP1, CASP8 and CASP10, suggesting that it may act as a transcription regulator; however the relevance of such function remains unclear.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: IFT57
• Uniprot:
  • Q9NWB7(Human)