HIPK2 Antibody (493918) [Alexa Fluor® 532]

• Reactivity: Hu
• Applications: ICC/IF
• Clone: 493918
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 532

HIPK2 Antibody (493918) [Alexa Fluor® 532] Summary

• Immunogen: E. coli-derived recombinant human HIPK2
  Met765-Ser924
  Accession # Q9H2X6
• Specificity: Detects human HIPK2 in direct ELISAs.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HIPK2 Antibody (493918) [Alexa Fluor® 532]

• DKFZp686K02111
• EC 2.7.11.1
• FLJ23711
• hHIPk2
• HIPK2
• homeodomain interacting protein kinase 2
• homeodomain-interacting protein kinase 2
• PRO0593
• Stank

Background

Homeodomain-interacting protein kinase-2 (HIPK2) is a serine/threonine kinase which interacts with transcription factors, including p53, CREB1, SMAD1, SMAD2, RUNX1 and others, and can act as a corepressor or coactivator depending on the transcription factor and subcellular localization.  Post-translational modifications control HIPK2 activity, including phosphorylation, small ubiquitin-like modifier modification, acetylation, and ubiquitination. HIPK2 regulation of cell proliferation makes it a key effector of proliferative diseases including cancer and kidney fibrosis. For example, HIPK2 can repress the proliferative Wnt-1/b-catenin pathway and HIF-1, promote apoptosis by activation of p53 and repression of delta Np63 alpha , MDM2 and CtBP, and its activity is enhanced by anti-cancer drugs such as cisplatin and etoposide.  HIPK2 oncorepession can be blocked by cytoplasmic localization, hypoxia, gene mutation and loss of heterozygosity.

 

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.