HINT2 Antibody (AT5B10) - BSA Free

Western Blot: HINT2 Antibody (AT5B10) [NBP2-59387] - The recombinant human HINT2 (50ng) and Celllysates (40ug) were resolved by SDS-PAGE,transferred to PVDF membrane and probed withanti-human HINT2 antibody (1:1000). Proteins werevisualized using a goat anti-mouse secondary antibodyconjugated to HRP and an ECL detection system.Lane 1. : HINT2 Recombinant protein Lane 2. :HepG2 cell lysate Lane 3. : U87MG cell lysate

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: AT5B10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

HINT2 Antibody (AT5B10) - BSA Free Summary

• Description: Novus Biologicals Mouse HINT2 Antibody (AT5B10) - BSA Free (NBP2-59387) is a monoclonal antibody validated for use in WB and ELISA. Anti-HINT2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Background: The recombinant human HINT2 (50ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HINT2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : HINT2 Recombinant protein Lane 2. : HepG2 cell lysate Lane 3. : U87MG cell lysate
• Immunogen: Recombinant human HINT2 (18-163aa) purified from E. coli
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: HINT2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for HINT2 Antibody (AT5B10) - BSA Free

• EC 3.-
• HINT-2
• HINT-3
• histidine triad nucleotide binding protein 2
• histidine triad nucleotide-binding protein 2, mitochondrial
• HIT-17
• HIT-17kDa
• PKCI-1-related HIT protein
• protein kinase C inhibitor-2

Background

Histidine triad proteins, such as HINT2, are nucleotide hydrolases and transferases that act on the alpha-phosphate of ribonucleotides (Brenner, 2002 [PubMed 12119013]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HINT2 Antibody (NBP2-59387)(1)

Publication using NBP2-59387

• Fan M, Chen Z, Huang Y et al. Overexpression of the histidine triad nucleotide-binding protein 2 protects cardiac function in the adult mice after acute myocardial infarction Acta Physiol (Oxf) 2020-01-03 [PMID: 31900976] (WB)

Bioinformatics

• Gene Symbol: HINT2