HGK/MAP4K4 Antibody [Alexa Fluor® 594]

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 594

HGK/MAP4K4 Antibody [Alexa Fluor® 594] Summary

• Immunogen: E. coli-derived recombinant human HGK
  Ser686-Val820
  Accession # O95819-4
• Specificity: Detects human HGK in Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HGK/MAP4K4 Antibody [Alexa Fluor® 594]

• EC 2.7.11
• EC 2.7.11.1
• FLH21957
• FLJ10410
• hepatocyte progenitor kinase-like/germinal center kinase-like kinase
• HGK
• HGKFLJ20373
• HPK/GCK-like kinase HGK
• KIAA0687
• MAP4K4
• MAPK/ERK kinase kinase kinase 4
• MEK kinase kinase 4
• MEKKK 4
• MEKKK4
• mitogen-activated protein kinase kinase kinase kinase 4
• Nck-interacting kinase
• NIKFLJ90111

Background

HGK (Hepatocyte kinase-like/Germinal center like-kinase Kinase; also MAP4K4) is a 140 kDa member of the MSN subfamily, MAP4K family of molecules. It is ubiquitously expressed, and intimately associated with TNF alpha signaling. HGK is activated by TNF‑induced c-Jun activation, and blocks both PPAR gamma expression and glucose-stimulated insulin secretion. Human HGK is 1239 amino acids (aa) in length (Accession # O95819) and contains one protein kinase domain (aa 25‑289) and a CHN region (aa 926‑1213). There are multiple splice variants. The antibody product offered by R&D Systems uses a 130 kDa, 1166 aa isoform as immunogen. It shows a deletion of aa 495‑525 and 570‑623, plus an eight aa insertion after His1112, and a GEV insertion after Ala740.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.