HGF R/c-MET [p Tyr1003] Antibody

Images

 
Western Blot: c-Met [p Tyr1003] Antibody [NSB882] - 293T cell extracts transiently transfected with human c-Met were resolved by SDS-PAGE on 10% Tris-glycine gel and transferred to PVDF. Membranes, after blocking ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

HGF R/c-MET [p Tyr1003] Antibody Summary

Immunogen
Synthetic phosphopeptide derived from the region of c-Met that contains tyrosine 1003.
Modification
p Tyr1003
Localization
Type I membrane protein
Specificity
Met (c-Met) [Tyr1003]
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MET
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1-1.0 ug/ml
Application Notes
WB: Detects a band of approximately 145 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Reactivity Notes

Cross-reacts with Human. Expected to cross-react with Mouse (100% identity with immunogen), Rat (100% identity with immunogen) due to sequence homology. Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Dulbecco's PBS (pH 7.3), 1.0 mg/ml and 50% Glycerol
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for HGF R/c-MET [p Tyr1003] Antibody

  • AUTS9
  • cMET
  • c-MET
  • EC 2.7.10
  • EC 2.7.10.1
  • hepatocyte growth factor receptor
  • HGF R
  • HGF receptor
  • HGF/SF receptor
  • HGFR
  • Met (c-Met)
  • met proto-oncogene (hepatocyte growth factor receptor)
  • met proto-oncogene tyrosine kinase
  • MET
  • oncogene MET
  • Proto-oncogene c-Met
  • RCCP2
  • Scatter factor receptor
  • SF receptor
  • Tyrosine-protein kinase Met

Background

Binding of scatter factor (SF)/hepatocyte growth factor (HGF) to the c Met receptor tyrosine kinase (RTK) triggers receptor dimerization and phosphorylation on multiple residues within the juxtamembrane, catalytic core and cytoplasmic tail domains, thereby regulating receptor internalization, catalytic activity and multisubstrate docking. c Met contains three tyrosines (Tyr-xx- x-Tyr-Tyr motif) within the activation loop of the catalytic domain. This is also seen with the insulin receptor, insulin-like growth factor receptor (IGF1R) and nerve growth factor (NGF) receptors/Trks, for which phosphorylation of all three tyrosines is required for full activation. With c Met (and the related family member, RON) phosphorylation of Tyr1234 and Tyr1235 have been shown to be important in receptor activation. Activation of the c Met receptor results in binding and/or phosphorylation of many intracellular signaling proteins including multiple adaptor proteins (e.g., Grb2, Shc, Cbl, Crk, cortactin, paxillin, and GAB1), and a variety of other signal transducers (e.g., PI 3-kinase, FAK, Src, ERK1&2, JNK1&2, PLC gamma, and STAT3). Tyr1003 is a negative regulatory phosphorylation site of c Met.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol MET
Entrez