HGF Activator Antibody (196817) - Pro form

• Reactivity: Hu
• Applications: WB, IP
• Clone: 196817
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

HGF Activator Antibody (196817) - Pro form Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Pro HGF Activator
  Gln36-Ser655
  Accession # Q04756
• Specificity: Detects human Pro HGF Activator in direct ELISAs and Western blots. It recognizes only the propeptide region of HGFA precursor, not active HGFA. In Western blots, 100% cross-reactivity with recombinant mouse Pro HGFA is observed and less than 5% cross-reactivity with recombinant human (rh) Factor X and rhThrombin is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: HGFAC
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HGF Activator Antibody (196817) - Pro form

• HGF Activator
• HGFA
• Hgfac

Background

Hepatocyte Growth Factor Activator (HGFA) is a serine endopeptidase that cleaves at the peptide bond between Arg494 and Val495 of single-chain human HGF precursor, generating the active heterodimer (1). HGFA is produced and secreted by the liver and normally circulates in the blood as an inactive zymogen (2, 3). The zymogen has a weak affinity for heparin but acquires a strong affinity for heparin upon activation that is linked to blood coagulation. This property may ensure the local action of this enzyme at the site of tissue injury (3). The 655 amino acid (aa) human HGFA precursor contains several predicted domains including a signal peptide (aa 1‑30), a propeptide (aa 31‑372), and a mature and active form (aa 373‑655) that is further processed into a short chain (aa 373‑407) and a long chain (aa 408‑655). The short chain and the long chain (catalytic domain) may form a disulfide-linked dimer. HGFA can be activated by thrombin or thermolysin (4). The active protease can be inhibited by HGFA inhibitors (HAIs). Two HAIs, HAI-1 and HAI-2, are known in mouse and human. HAI-1 is not only an inhibitor, but also a specific acceptor of active HGFA, acting as a reservoir of this enzyme on the cell surface (5).

1. Kitamura, N. (2004) in Handbook of Proteolytic Enzymes (Barrett, A.J. et al. Eds.) p. 1712, Academic Press, San Diego.
2. Miyazawa, K. et al. (1993) J. Biol. Chem. 268:10024.
3. Miyazawa, K. et al. (1996) J. Biol. Chem. 271:3615.
4. Shimomura, T. et al. (1993) J. Biol. Chem. 268:22927.
5. Kataoka, H. et al. (2000) J. Biol. Chem. 275:40453.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HGFAC
• Uniprot:
  • Q04756()