HGD Antibody (7O8Q1)

Western Blot: HGD Antibody (7O8Q1) [NBP3-15477] - Western blot analysis of extracts of various cell lines, using HGD antibody (NBP3-15477) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

• Reactivity: Hu
• Applications: WB
• Clone: 7O8Q1
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

HGD Antibody (7O8Q1) Summary

• Description: Novus Biologicals Rabbit HGD Antibody (7O8Q1) (NBP3-15477) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 346-445 of human HGD (Q93099). IRGHYEAKQGGFLPGGGSLHSTMTPHGPDADCFEKASKVKLAPERIADGTMAFMFESSLSLAVTKWGLKASRCLDENYHKCWEPLKSHFTPNSRNPAEPN
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: HGD
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500 - 1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol, 0.05% BSA
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for HGD Antibody (7O8Q1)

• AKU
• EC 1.13.11.5
• HGOFLJ94126
• homogentisate 1,2-dioxygenase (homogentisate oxidase)
• homogentisate 1,2-dioxygenase
• homogentisate oxidase
• Homogentisate oxygenase
• Homogentisic acid oxidase
• homogentisicase

Background

Homogentisate 1,2-dioxygenase (HGD) gene mutations are the molecular cause of alkaptonuria, a rare hereditary disorder of the phenylalanine catabolism. The highest expression of HGD is in the prostate, small intestine, colon, and liver. The HGD gene contains 14 exons. Conflicting reports have placed the gene at 3q2, 3q13.3-q21, 3q21-q24, 3q21-q23, or 3q25-q26.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HGD