Hexokinase 2 Antibody (4H1) - Azide and BSA Free

Western Blot: Hexokinase 2 Antibody (4H1) [H00003099-M01] - HK2 monoclonal antibody (M01), clone 4H1. Analysis of HK2 expression in A-431.

Western Blot: Hexokinase 2 Antibody (4H1) [H00003099-M01] - HK2 monoclonal antibody (M01), clone 4H1. Analysis of HK2 expression in PC-12.

Sandwich ELISA: Hexokinase 2 Antibody (4H1) [H00003099-M01] - Detection limit for recombinant GST tagged HK2 is approximately 0.3ng/ml as a capture antibody.

• Reactivity: Hu, Rt
• Applications: WB, ELISA
• Clone: 4H1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Hexokinase 2 Antibody (4H1) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: HK2 (AAH21116, 818 a.a. ~ 917 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. IVKEVCTVVARRAAQLCGAGMAAVVDRIRENRGLDALKVTVGVDGTLYKLHPHFAKVMHETVKDLAPKCDVSFLQSEDGSGKGAALITAVACRIREAGQR
• Specificity: HK2 - hexokinase 2 (4H1)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: HK2
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Hexokinase 2 Antibody (4H1) - Azide and BSA Free

• DKFZp686M1669
• EC 2.7.1
• Hexokinase 2
• Hexokinase type II
• HK2
• HKII
• HXK2
• Muscle form hexokinase
• muscle

Background

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Hexokinase 2 Antibody (H00003099-M01)(5)

Publications using H00003099-M01

• Pamela F, Giacomo L, Federica F et al. Hindering NAT8L expression in hepatocellular carcinoma increases cytosolic aspartate delivery that fosters pentose phosphate pathway and purine biosynthesis promoting cell proliferation. Redox Biol. 2022-12-24 [PMID: 36580805]
• Serena C, Fabio C, Pamela F et al. Adaptive antioxidant response to mitochondrial fatty acid oxidation determines the proliferative outcome of cancer cells. Cancer Lett. 2022-11-17 [PMID: 36402229]
• Serena C, Fabio C, Daniela T et al. ROS-dependent HIF1? activation under forced lipid catabolism entails glycolysis and mitophagy as mediators of higher proliferation rate in cervical cancer cells. J Exp Clin Cancer Res. 2021-03-11 [PMID: 33706793]
• Desideri E, Vegliante R, Cardaci S et al. MAPK14/p38alpha-dependent modulation of glucose metabolism affects ROS levels and autophagy during starvation. Autophagy 2014-09-01 [PMID: 25046111] (WB)
• Richter S, Richter JP, Mehta SY et al. Expression and role in glycolysis of human ADP-dependent glucokinase. Mol Cell Biochem 2012-01-05 [PMID: 22219026]

Bioinformatics

• Gene Symbol: HK2
• Entrez:
  • 3099(Human)
• Uniprot:
  • P52789()